POLYMORPHISM OF GROWTH HORIVIONE GENE IN INDONESIAN CATTLE

Abstract

The study was conducted to investigate the polymorphism of growth hormone
(GH) gene in Indonesian cattle. If the polymorphic characteristic of this gene could be detected then its effect on growth could be studied and then it can be used as the genetic marker for selection of growth rate in Indonesian cattle. Blood samples were taken from three population of Bali cattle, Bali Improvement Project (P3Bali), Bali island, and South Kalimantan, and were lll, 85 and 46 samples respectively. Besides that blood samples were taken from population of Ongole grade (PO), Simmental x PO (SIMPO) and Limousine x PO (LIMPO) cattle in Special District of Yogyakarta, and were 43, 44 and 42 samples respectively. The identification of GH gene polymorphism was conducted by digested the DNA fragment of 221 bp extended from the fourth intron region (49 bp) to fifth of exon (162 bp) by Alul enzyme. The results indicated that in the three Bali cattle populations only L allele was found, and no V allele or other alleles of GH gene, while the PO, SIMPO dan LIMPO cattle populations indicated that two alleles: L and V were found at the GH gene with higher allelic frequency for L allele. Its was concluded that the GH gene was not polymorphic in Bali cattle and the genotype of GH was homozygote LL, while the GH gene was found polymorphic in PO, SIMPO dan LIMPO cattle and the genotypes of GH were LL dan LV.