THE EFFECT OF VITAMIN E SUPPLEMENTATION IN RATION CONTAINING MANHADDEN FISH AND KERNEL PALM OILS TO NATIVE CHICKEN’S SPERM QUALITY AND HISTOLOGICAL PROFILE OF TESTES

Abstract

The experiment was conducted to determine the effect of manhadden fish and kernel
palm oils and their combination with different levels of vitamin E on the sperm quality (mortality, abnormality, motility, konsentration), and histological profile of testes. This research was done in Laboratory of Animal Feed Department of Animal Feed and Nutrition Gl\/IU_ Yogyakarta; Laboratory of Animal Feed and Nutrition and
Experimental Sub-station Faculty of Animal Sciences UNSOED, Purwokerto. The Research consisted of two steps of experiment, and was carried out from April 2004 to November 2005. Chickens used in this research consist of 20 cockerels of 22 weeks old. The rations at the first step were: RQ = 0% manhadden fish oil and 0% kernel palm oil; R1 = l0% manhadden fish oil and 0% kernel palm oil; R; = 5% manhadden fish oil and 5% kernel palm oil; R3 I 0% manhadden fish oil and 10% kernel palm oil. The second step: R31 = basal feed + 0 mg vitamin E /kg of feed; R3; = basal feed + 10 mg Vitamin E/kg of feed; R3; I basal feed + 20 mg Vitamin E /kg of feed; R34 = basal feed + 30 mg Vitamin E /kg of feed. Research was done use Completely Randomized Design (CRD), each treatment was repeated 6 times. Data then were analyzed using analysis of varian continued with Duncan Test. The result showed that the first step rationof affeccted spenn motility and concentration, but very significantly (P<0.0l) not significantly (P>0.05) affected mortality and abnormality of sperm. The second step of study showed that the treatment affected abnormality, motility and concentration of sperm very significantly _(P<0.0l), and significantly (P<0.05) affected spenn mortality. It was concluded that vitamin E supplementation in feed containing manhadden fish and kernel palm oils improved the sperm quality (improved motility and concentration, reduced mortality and abnormality) and improve the testicular development.