VITRIFICATION OF BOVINE OOCYTE USING DIFFERENT INTRACELLULAIR CRYOPROTECTANTS

Abstract

The aim of this research was to find out the effect of intracellulair cryoprotectant Ethylene Glycol (EG)_(_‘ilycerol (Gly)and DMSO (Dimethylsulfoxide) on normal oocyte’s morphology, viability and the rate of oocyte’s fertilisation after vitrification. Medium of vitritication tested was : 40% EG + 0.5 M Sucrosa (VSi), 40% GLY + 0.5 M Sucrosa (\/S3). 40% DMSO + 0.5 M Sucrosa (VS3). The percentage of oocyte with normal morphology after that had been vitrificated in VS1 solution was obviously higher (p < 0,05) than if it was in VS; and VS; solution. The vitrification of oocyte within a solution with 40% of ctylen-glicol (VSI) showed higher viability (p < 0.05) than another of two solution ol‘\'itrif1cation. The vitrification process of MT II oocyte in VS; was showing different significantly (P<0,05) by control oocyte to viability and
fertilization rate. After \itrification process into VS], VS2, or VS;, GV-oocyte showed a lower viability (P<0.05) than GVBD, metaphase I(Mt I) and metaphase II(Mt II). The in virro fertilization rate of MT-II oocyte after vitrification in VS1(8l.25%) was higher than VS] (68.73%) and VS; (66.25%). It was concluded that nomial oocyte’s
morphology. viability and the rate of oocyte’s fertilisation after vitrification in VS1 was better than those were in VS; and VS3. GV-oocyte was more sensitive to vitrification solution than it was at (i\’l3D_ MT I and MT II.The rate of viability of Mt-I and Mt-II Oocyte after \ itrilication were higher than GV and GVBD’s ones.