APPLICATION OF CELLULOLYTIC MIXED CULTURES FOR RUMINANT PRODUCTIVITY 3 THE EFFECT OF CELLULOLYTIC MIXED CULTURES INOCULATION ON CELLULASE ACTIVITY AND VOLATILE FATTY ACID PRODUCTION OF CATTLE RUMEN FLUID BY IN VITRO ANALYSIS

Abstract

The objective of this experiment was determined the effects of cellulolytic mixed
cultures (CMC) inoculation on cellulase activity and Volatile fatty acid (VFA) production of cattle rumen by in vitro analysis. CMC as starter having high activity of endo-glucanase and B-glucosidase were obtained by isolation and selection of rumen microbes from buffalo. Rumen fluid (RF) as microbial donor was taken from rumen canullated cattle that were fed 60% rice straw and 40% concentrate for l5 days. Series of different kind ratio between CMC: RF as starter for in vitro rice straw fermentation (treatments) were designed using one way completely
randomized design as follow: l) CMC100 (l00% of CMC: 0% of RF); 2) CMCg() (80% of CMC: 20% of RF); 3) CMC6() (60% of CMC: 40% of RF); CMC4() (40% of CMC: 60% of RF); 5) CMCZQ (20% of CMC1 80% of RF); 6) CMC0 (0% of CMC: 100% of RF). All treatments have 3 replications and were incubated at 39 C for 48 hours. Different variable was determined by Duncan’s new multiple range test. The result of this study showed that CMC inoculation in rice straw fermentation by RF gave effect (P< .05%) on cellulase activity and VFA production. The highest activity of endo-glucanase and B-glucosidase activity namely were in CIVlC8() (80% of CMC: 20% of RF) and CMC1()0 (l00% of CMC: 0% of RF) respectively. While the highest concentration acetate, propionate and butyrate produced was CMCgQ (80% of CMC: 20% of RF). Finally the starter having ratio of 80% of CMC: 20% of RF (CMC3Q) showed the best treatments.