DEVELOPMENT OF ROUGH MUTANT BRUCELLA ABORTUS VACCINE FROM LOCAL FIELD STRAIN TO ERADICATE BRUCELLOSIS IN INDONESIA



Susan Maphilindawati Noor(1*), Darodjat Darodjat(2), Enuh Rahardjo(3)

(1) Research Institute for Veterinary Science, Jl. R.E. Martadinata 30 Bogor, Indonesia. Ph: (0251) 334456, 331048,
(2) Research Institute for Veterinary Science, Jl. R.E. Martadinata 30 Bogor, Indonesia. Ph: (0251) 334456, 331048,
(3) Pusat Veterinary Farma (Pusvetrna), Surabaya, Indonesia.
(*) Corresponding Author

Abstract


Brucellosis in cattle causes abortion and infertility. B. abortus strain 19 (S19)
vaccine has been used to prevent bovine brucellosis. However, the S19 vaccine
induces abortion in pregnant cattle and persistent infection after vaccination, which
is indistinguishable by brucellosis surveillance testing from those productions by
natural infection with field strains. The study reported here was designed to develop
a rough mutant B. abortus, named strain RB27, which was derived from virulent
local field strain‘ The RB27 was derived by repeated passage of smooth strain 158
on Trypticase soy supplemented with 1.5% bacto agar (TSBA) and varying
concentrations rifainpin. In order to stabilise the putative mutation, the RB 27 was in
vitro passaged in guinea pigs as well as in vitra passaged on unsuppleniented TSBA
plates. The result showed that the RB 27 strain did not revert to smooth forrn and did
not change in its virulence.


Keywords


Brucella, Abortus, Mutant, Local strain, Vaccine, Rifampin

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