THE EFFECT OF EXTENDERS AND GLYCEROL LEVEL TO THE QUALITY OF AFTER FREEZING PRIANGAN RAM SPERM

Abstract

A study on the effect of five extenders (Whole Milk, Skim Milk, Egg Yolk
Citrate and Egg Yolk Tris) and three different level of glycerol (7.5 %, l0 %, and
12.5 %) has been conducted at Animal Reproduction Laboratory, Faculty of Animal
Husbandry, Padjadjaran University, Jatinangor. The objectives of this study were to
find out the efiect of semen extenders, glycerol level, and the combination of both to
the semen quality of after freezing of Piiangan rarn sperm. This study used five -
1.5 years old - of Priangan rams. Theywere kept in individual pen and fedbyfield grass and dairy cattle concentrate. Semen were collected by artificial vagina and evaluated macroscopic and microscopically. Semen was extended in two stages, and glycerol was added gradually in 5° C. Semen was frozen manually in liquid nitrogen on - 196° C. Evaluation of afier thawed semen quality has been done 24 hours later. The evaluated variables were sperm motility and sperm abnormality. The study used
Completely Randomised Design with 5 x 3 factorial pattern. The collected data were
analyzed with Analysis of Variance and followed by Duncan’s New Multiple-Range
Test The result of this study shows that factors of extenders, glycerol level, and the
combination of both are highly significant (P<0.0l) affect the quality of alter
freezing Priangan ram semen. The combination between Egg Yolk Tris and 7.5 % or
10 % of glycerol level is the best semen extender on maintaining the quality (sperm
motility and sperm abnormality) of alter freezing Priangan ram semen.