Distilled liquid smoke as feed fat protector and it’s effect on fatty acids in rumen fluid

Abstract

This study aims to determine the effect of dietary fat protection using distillated
liquid smoke on rumen fluid fatty acids. Crude palm oil (CPO) as a source of feed fat, mixed
with skim milk (1:2), then divided into 3 parts: without protected by distillated liquid smoke
(P0) and protected by distillated liquid smoke 2,5% (P1) and 5% (P2). For the in vitro test,
rumen fluid was used as a microbial donor with elephant grass and soybean meal (60:40) as
substrate. A total of 5% CPO was put in a syringe containing 30 ml of rumen fluid, substrate
and buffer, then anaerobically fermented at 39 o C for 48 hours. The parameters observed were
rumen fluid fatty acids. This study used a completely randomized design, with 3 treatments
(P0, P1, P2) each of 3 replications. The results showed that the source of feed fat protection
(CPO) used distilled liquid smoke, increasing (P<0.01) fatty acid composition of rumen fluid
fermented. P1 was better at increasing (P<0.01) unsaturated fatty acids in rumen fluids. It can
be concluded that liquid smoke can be used as a feed fat protector, because it can reduce
hydrogenation and increase unsaturated fatty acids in rumen fluids in vitro fermented.