Jurnal Anatomi Indonesia
Karakteristik dan komposisi semen kancil (Tragulus javanicus) yang dikoleksi dengan elektroejakulator
Wahono Esthi Prasetyaningtyas(1), Mohamad Agus Setiadi(2), Mokhamad Fahrudin(3), Abdul Wahid Haron(4), Srihadi Agungpriyono(5*)
(1) DepartemenAnatomi, Fisiologi dan Farmakologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Bogor
(2) Departemen Klinik Reproduksi dan Kebidanan, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Bogor
(3) DepartemenAnatomi, Fisiologi dan Farmakologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Bogor
(4) Department of Clinical Studies, Faculty of VeterinaryMedicine, University of Putra Malaysia, 43400 UPM Serdang, Selangor,Malaysia
(5) DepartemenAnatomi, Fisiologi dan Farmakologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Bogor
(*) Corresponding Author
Abstract
The ejaculates were taken by electroejaculation from four apparently healthy young adultmale lessermouse
deer (Tragulus javanicus). The animals were anesthetized with a combination of xylazin and ketamine followed
by ether per inhalation anesthesia. The semen was white, yellowish, or creamy in color. The semen had a pH of
7.63±0.22. Themean values for volumewas 19.44±6.8 μl, spermconcentration was 47.44 ± 4.9 x 106 sperm/ml,
percentage of sperm motility was 36.43±1.1 %, percentage of live sperm was 53.11±3.0 %, percentage of
abnormal spermatozoa was 21.03±1.05%and percentage of intact acrosome was 52.28±2.7%, respectively.
These values were relatively lowwhen compared to other domestic ruminants and suggested to be relatedwith
age and sexualmaturity of the animal.The seminal plasma contained 10.2–11.5mg/100ml fructose, 22.07–24.5
mg/100ml citric acid, 65mg/100ml proteins, 22.07–24.5mg/100ml sorbitol, 91.1– 94.72mg/100ml sodium, 0.1mg/
100ml potassium, 12.8mg/100ml calcium, 0.8mg/100mlmagnesiumand 10.72–11.2 chloride. By SDS PAGE,
eleven proteins with different molecular weight were determined in the seminal plasma. Among them, the
proteins with 64.77 kDa and 71.72 kDa were particularly prominent.
deer (Tragulus javanicus). The animals were anesthetized with a combination of xylazin and ketamine followed
by ether per inhalation anesthesia. The semen was white, yellowish, or creamy in color. The semen had a pH of
7.63±0.22. Themean values for volumewas 19.44±6.8 μl, spermconcentration was 47.44 ± 4.9 x 106 sperm/ml,
percentage of sperm motility was 36.43±1.1 %, percentage of live sperm was 53.11±3.0 %, percentage of
abnormal spermatozoa was 21.03±1.05%and percentage of intact acrosome was 52.28±2.7%, respectively.
These values were relatively lowwhen compared to other domestic ruminants and suggested to be relatedwith
age and sexualmaturity of the animal.The seminal plasma contained 10.2–11.5mg/100ml fructose, 22.07–24.5
mg/100ml citric acid, 65mg/100ml proteins, 22.07–24.5mg/100ml sorbitol, 91.1– 94.72mg/100ml sodium, 0.1mg/
100ml potassium, 12.8mg/100ml calcium, 0.8mg/100mlmagnesiumand 10.72–11.2 chloride. By SDS PAGE,
eleven proteins with different molecular weight were determined in the seminal plasma. Among them, the
proteins with 64.77 kDa and 71.72 kDa were particularly prominent.
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