Peningkatan Viabilitas dan Vigor Benih Kakao (Theobroma cocoa L.) Menggunakan Campuran Rizobakteri dan Mikoriza
Faradilla Faradilla(1), F. Silvi Dwi Mentari(2), Nur Hidayat(3), Reza Wahyudi(4), La Mudi(5*), Gusti Ayu Kade Sutariat(6)
(1) Program Studi Budidaya Tanaman Perkebunan, Politeknik Pertanian Negeri Samarinda
(2) Program Studi Budidaya Tanaman Perkebunan, Politeknik Pertanian Negeri Samarinda
(3) Program Studi Budidaya Tanaman Perkebunan, Politeknik Pertanian Negeri Samarinda
(4) Program Studi Budidaya Tanaman Perkebunan, Politeknik Pertanian Negeri Samarinda
(5) Politeknik Pertanian Negeri Samarinda
(6) Jurusan Agroteknologi, Fakultas Pertanian, Universitas Halu Oleo
(*) Corresponding Author
Abstract
Penggunaan benih bermutu rendah akan berdampak terhadap penurunan produksi tanaman. Oleh karena itu, guna mendukung peningkatan mutu benih tanaman agar menghasilkan bibit yang bermutu tinggi. Penggunaan rizobakteri dan mikoriza secara tunggal telah mampu memberikan efek yang signifikan dalam mendukung pertumbuhan dan hasil tanaman. Penelitian ini bertujuan untuk meningkatkan viabilitas dan vigor benih kakao menggunakan campuran rizobakteri dan mikoriza. Penelitian ini dilaksanakan pada bulan Juni sampai Juli 2022 yang bertempat di Laboratorium Agronomi Program Studi Budidaya Tanaman Perkebunan Politeknik Pertanian Negeri Samarinda. Penelitian ini menggunakan rancangan acak lengkap dengan 9 perlakuan dan setiap perlakuan diulang 3 kali sehingga diperoleh 27 unit percobaan. Variabel pengamatan dari penelitian ini meliputi: daya berkecambah (%), keserempakan tumbuh (%), indeks vigor (%), kecepatan tumbuh relatif (% etmal-1) dan T50 (hari). Data hasil pengamatan dianalisis menggunakan analisis ragam (Anova). Hasil analisis yang menunjukkan pengaruh nyata dilanjutkan dengan uji Beda Nyata Terkecil (BNT)α=0,05. Hasil penelitian menunjukkan bahwa campuran rizobakteri dan mikoriza terbukti efektif meningkatkan perkecambahan bibit tanaman kakao. Perlakuan campuran rizobakteri isolat P01 dan dosis mikoriza 50 g memberikan hasil terbaik terhadap peningkatan daya berkecambah hingga mencapai 33.33%, indeks vigor sebesar 100.05%, keserempakan tumbuh sebesar 52,17%, kecepatan tumbuh relatif sebesar 46.21% dan T50 sebesar 25.03% bila dibandingkan dengan tanpa rizobakteri dan tanpa mikoriza (R0M0). Berdasarkan hal ini tentunya, campuran rizobakteri dan mikoriza dapat digunakan untuk meningkatkan pertumbuhan bibit tanaman kakao.
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Begum, N., C. Qin, M. A. Ahannger, R. Raza, M. I. Khan, M. Ashraf, N. Ahmed, & L. Zhang. 2019. Role of arbuscular mycorrhizal fungi in plant growth regulation: implications in abiotic stress tolerance. Front. Plant Sci., 10:1068. doi: 10.3389/fpls.2019.01068.
Bizos, G., E. M. Papatheodorou, T. Chatzistathis, N. Ntalli, V. G. Aschonitis, & N. Monokrousos, N., 2020. The role of microbial inoculants on plant protection, growth stimulation, and crop productivity of the olive tree (Olea europaea L.). Plants, 9: 743. doi:10.3390/plants9060743.
Chandini, P., R. Ashwin and D. J. Bagyaraj. 2022. Dual inoculation with AM fungus Funneliformis mosseae and PGPR Bacillus sonorensis enhances growth of brinjal seedlings raised in pro trays. Kavaka, 58(3): 11-15. doi: 10.36460/Kavaka/58/3/2022/11-15.
Chen, W., P. Meng, H. Feng, and C. Wang. 2020. effects of arbuscular mycorrhizal fungi on growth and physiological performance of Catalpa bungei C.A.Mey. under drought stress. Forests, 11: 1117. doi:10.3390/f11101117.
Dhifa, P. A., S. Syamsuddin, & Hasanuddin, H. (2021). Pengaruh perlakuan benih menggunakan rizobakteri pemacu pertumbuhan tanaman terhadap pertumbuhan dan produksi tanaman okra (Abelmoschus esculentus L. Moench). J. Agrista, 25(2), 73-81.
Dhiman, M., L. Sharma, P. Kaushik, A. Singh, & M. M. Sharma. 2022. Mycorrhiza: an ecofriendly bio-tool for better survival of plants in nature. Sustainability, 14: 10220. https://doi.org/10.3390/su141610220.
Disbun-Kaltim. 2022. Produksi Tanaman Kakao Kalimantan Timur. Kalimantan Timur. Samarinda.
Fernandez-Zarate, F. H., A. E. Huaccha-Castillo, L. Quiñones-Huatangari, S. P. Vaca-Marquina, T. Sanchez-Santillan, E. Morales-Rojas, A. Seminario-Cunya, M. Guelac-Santillan, L. M. Barturén-Vega, & D. Coronel-Bustamante. 2022. Effect of arbuscular mycorrhiza on germination and initial growth of Cinchona officinalis L. (Rubiaceae), Forest Science and Technology. 18(4):182-189. DOI:10.1080/21580103.2022.2124318.
Foo, E., J. J. Ross, W. T. Jones, & J. B. Reid. 2013. Plant hormones in arbuscular mycorrhizal symbioses: an emerging role for gibberellins. Ann Bot., 111(5):769-79. doi: 10.1093/aob/mct041. Epub 2013 Mar 18. PMID: 23508650; PMCID: PMC3631329.
Hashem, A., A. A. Alqarawi, R. Radhakrishnan, A. F. Al-Arjani, H. A. Aldehaish, & D. Egamberdieva. 2018. Arbuscular mycorrhizal fungi regulate the oxidative system, hormones, and ionic equilibrium to trigger salt stress tolerance in Cucumis sativus L. Saudi J. Biol. science. 25(6): 1102–1114. doi: 10.1016/j.sjbs.2018.03.009.
Hasid, R., A. M. Kandari, Halim, M. J. Arma, Sarawa, & M. Yusuf. 2020. Effect of arbuscular mycorrhizal and sago dregs on peanut plants (Arachis hypogaea L.) grown on southeast sulawesiʼs dryland. J. Agron., 19: 40-45. DOI: 10.3923/ja.2020.40.45.
Hasid, R., M. J. Arma, & A. Nurmas. 2018. Existence arbuscula mycorrhiza and its application effect to several variety of corn plant (Zea mays L.) In marginal dry land. Pak. J. Biol. Sci. 21: 199-204. DOI: 10.3923/pjbs.2018.199.204.
Herawati, A., J. Syamsiyah, Mujiyo, M. Rochmadtulloh, A. A. Susila, & M. R. Romadhon. 2021. Mycorrhizae and a soil ameliorant on improving the characteristics of sandy soil. Sains Tanah – Journal of Soil Science and Agroclimatology.18(1): 73-80.
Hidayat, C., D. H. Arief, J. Sauman, & A. Nurbaity. 2019. Microaggregate and macroaggregate of Andisol affected by arbuscular mycorrhizal fungi and rhizobacteria. IOP Conference Series: Earth and Environmental Science. 334. 012025. Doi: 10.1088/1755-1315/334/1/012025.
Igiehon, N. O, and O. O. Babalola. 2017. Biofertilizers and sustainable agriculture: exploring arbuscular mycorrhizal fungi. Appl Microbiol Biotechnol. 101:4871–4881. DOI 10.1007/s00253-017-8344-z.
Jabborova, D., K. Annapurna, A. Azimov, S. Tyagi, K. R. Pengani, P. Sharma, V. K. Vikram, P. Poczai, O. Nasif, M. J. Ansari, & R. Z. Sayyed. 2022. Co-inoculation of biochar and arbuscular mycorrhizae for growth promotion and nutrient fortification in soybean under drought conditions. Front. Plant Sci. 13:947547. doi:10.3389/fpls.2022.947547.
Kalamulla, R., S. C. Karunarathna, S. Tibpromma, M. C. A. Galappaththi, N. Suwannarach, S. L. Stephenson, S. Asad, Z. S. Salem, & N. Yapa. Arbuscular mycorrhizal fungi in sustainable agriculture. Sustainability. 14: 2250. https://doi.org/10.3390/su141912250
Leontidou, K., S. Genitsaris, A. Papadopoulou, N. Kamou, I. Bosmali, T. Matsi, P. Madesis, D. Vokou, K. Karamanoli, & I. Mellidou. 2020.. Plant growth promoting rhizobacteria isolated from halophytes and drought-tolerant plants: genomic characterization and exploration of phyto-benefcial traits. Scientific Reports. 10: 14857.
Lestari, S. D., N. Augustien, & I. R. Moeljani. 2020. Respon pertumbuhan bibit kawista (Limonia acidissima L.) terhadap pemberian pgpr (plant growth promoting rhizobacteria). Plumula. 8(2): 93-100.
Liu, C. Y., F. Zhang, D. J. Zhang, A. K. Srivastava, Q. S. Wu, & Y. N. Zou. 2018a. Mycorrhiza stimulates root-hair growth and iaa synthesis and transport in trifoliate orange under drought stress. Sci Rep. 8: 1978. https://doi.org/10.1038/s41598-018-20456-4.
Liu, C. Y., P. Wang, D. J. Zhang, & Z. Ying-Ning. 2018b. Mycorrhiza-induced change in root hair growth is associated with iaa accumulation and expression of EXPs in trifoliate orange under two P levels. Scientia Horticulturae. 234: 227-235. DOI:10.1016/j.scienta.2018.02.052.
Meena, M., P. Swapnil, K. Divyanshu, S. Kumar, Harish, Y. N. Tripathi, A. Zehra, A. Marwal, & R. S. Upadhyay. 2020. Review: pgpr‐mediated induction of systemic resistance and physiochemical alterations in plants against the pathogens: current perspectives. Journal of Basic Microbiology. 1-34. DOI: 10.1002/jobm.202000370.
Mudi, L., & Rusmini. 2022. Eksplorasi Rhizobakteri Indigenos dari Rizosfer Tanaman Kelapa Sawit (Elais guinensis Jack.) dan Uji Potensinya dalam Meningkatkan Vigor Benih. Laporan Akhir Penelitian.
Munarti, A. Wulan, & A. Utami. 2018. Exploration and identification of arbuscular mycorrhizal fungi from the rhizosphere of chili plants (Capsicum annuum L.) in Bogor. Journal of Science Innovare. 1(2): 50-53.
Pan, X., J. Zhang, Z. Xue, J. Liang, Y. Chen, & Y. Liu. 2022. Synergistic effect of phytohormone-producing ectomycorrhizal fungus Suillus luteus and fertilizer GGR6 on Pinus massoniana growth. Journal of Plant Interactions. 17(1): 643-655. DOI: 10.1080/17429145.2022.2081369.
Pérez-de-Luque, A., S. Tille, I. Johnson, D. Pascual-Pardo, J. Ton, & D. D. Cameron. 2017. The interactive effects of arbuscular mycorrhiza and plant growth-promoting rhizobacteria synergistically enhance host plant defences against pathogens. Sci Rep. 7: 16409. https://doi.org/10.1038/s41598-017-16697-4.
Pons, S., S. Fournier, C. Chervin, G. Bécard, & S. Rochange. 2020. Phytohormone production by the arbuscular mycorrhizal fungus Rhizophagus irregularis. PLOS ONE 15(10): e0240886. https://doi.org/10.1371/journal.pone.0240886.
Poštić, D., R. Štrbanović, A. Stanojković-Sebić, M. Tabaković, M. Milivojević, S. Jovanović, & R. Stanisavljević, 2019. Increasing the pepper seed quality using mycorrhiza fungi. Journal on Processing and Energy in Agriculture. 23(2): 66-68.
Raklami, A., N. Bechtaoui, A. Tahiri, A. Slimani, A. Bargaz, A. Meddich, & K. Oufdou. 2021. Co-inoculation with rhizobacteria and mycorrhizae can improve wheat/faba bean intercrop performance under field conditions. Front. Agron. 3:734923. doi: 10.3389/fagro.2021.734923.
Raklami, A., N. Bechtaoui, A. Tahiri, M. Anli, A. Meddich, & K. Oufdou. 2019. Use of rhizobacteria and mycorrhizae consortium in the open field as a strategy for improving crop nutrition, productivity, and soil fertility. Front. Microbiol. 10:1106. doi: 10.3389/fmicb.2019.01106.
Sadjad, S., Murniati, E. & S. Ilyas. 1999. Parameter Pengujian Vigor Benih dari Komparatif ke Simulatif (p.185). Grasindo. Jakarta.
Sari, H. P., W. Warnita, & I. Dwipa. 2019. Pemberian rizobakteri dan coumarin pada pertumbuhan dan pembentukan umbi tanaman kentang (Solanum tuberosum L.). J. Agron. Indonesia. 47(2): 188-195.
Shaikh, S. S., S. J. Wani, and R. Z. Sayyed. 2018. Impact of interactions between rhizosphere and rhizobacteria: a review. J. Bacteriol Mycol. 5(1): 1058.
Singh, N., and D. Singh. 2020. Effect of plant growth promoting bacteria on seed germination, seedling vigor, and growth Lagenaria siceraria (Molina) Standl. Int.J.Curr.Microbiol.App.Sci. (2020) 9(8): 1161-1168. https://doi.org/10.20546/ijcmas.2020.908.128.
Singh, T. B., V. Sahai, D. Goyal, M. Prasad, A. Yadav, P. Shrivastav, A. Ali, & P. K. Dantu. 2020. Identifcation, characterization, and evaluation of multifaceted traits of plant growth promoting rhizobacteria from soil for sustainable approach to agriculture. Current Microbiology. https://doi.org/10.1007/s00284-020-02165-2.
Sudewi, S., B. Patandjengi, A. R. Saleh, A. Yani, & R. Ratnawati. 2021. Eksplorasi rizobakteri penghasil giberelin dari padi lokal aromatik, Sulawesi Tengah. Prosiding Seminar Nasional Politeknik Pertanian Negeri Pangkajene Kepulauan, 310-316. Retrieved from https://ojs.polipangkep.ac.id/index.php/proppnp/article/view/149.Sutariati, G. A. K., N. M. Rahni, L. Mudi, Nurlina, Hamriani, D. N. Yusuf, Muhidin, & Zahrima. 2020. Isolation and screening test of indigenous endophytic bacteria from areca nut rhizosphere as plant growth promoting bacteria. IOP Conference Series: Earth and Environmental Science. 454(1): 012187 (2020).
Sutariati, G.A.K., Muhidin, N.M. Rahni, L. Mudi, R.R. Maharani, & G.N.A. Wibawa. 2021. The effectiveness of endo-rhizo bacteria isolated from areca nut rizosphere (Areca cathecu L.) in breaking dormancy and improvement of seed vigor. IOP Conf. Ser.: Earth Environ. Sci. 807 042039.
Sutariati, G. A. K., A. Khaeruni, Muhidin, A. Madiki, T. C. Rakian, L. Mudi, & N. Fadillah. 2019. Seed biopriming with indigenous endophytic bacteria isolated from Wakatobi rocky soil to promote the growth of onion (Allium ascalonicum L.). IOP Conference Series: Earth and Environmental Science. 260. Doi:10.1088/1755-1315/260/1/012144.
Tang, A., A. O. Haruna, N. M. A. Majid, & M. B. Jalloh. 2020. Potential pgpr properties of cellulolytic, nitrogen-fixing, phosphate-solubilizing bacteria in rehabilitated tropical forest soil. Mcroorganisms. 8: 442. doi:10.3390/microorganisms8030442.
Widowati, T., T. K. Dewi, S. J. R. Lekatompessy, & S. Antonius. 2020. Pengaruh pupuk hayati berbasis jamur mikoriza arbuskular dan rhizobakteri pemacu pertumbuhan tanaman terhadap pertumbuhan bibit kakao (Theobroma cocoa L.). Jurnal Agro Industri Perkebunan. 8(1): 33. DOI:10.25181/jaip.v8i1.1371.
Yanti, Y., Warnita, Reflin, & H. Hamid. 2018. Short Communication: development of selected pgpr consortium to control Ralstonia syzygii subsp. Indonesiensis and promote the growth of tomato. Biodiversitas. 19(6): 2073-2078. DOI: 10.13057/biodiv/d190612.
Zhang, T., Y. Hub, K. Zhang, C. Tian, & J. Gu. 2018. Arbuscular mycorrhizal fungi improve plant growth of Ricinus communis by altering photosynthetic properties and increasing pigments under drought and salt stress. Eng. Crop. Prod. 117: 13–19. doi:10.1016/j.indcrop.2018.02.087.
DOI: https://doi.org/10.22146/veg.81318
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