Frozen Biofloc Biomass as A Functional Feed Supplement Enhances Production Performance of Mexican Dwarf Crayfish (Cambarellus patzcuarensis)

https://doi.org/10.22146/jfs.115523

Ujang Subhan(1*), Silmi Aulia Kamilah(2), Lantun Paradhita Dewanti(3), Irfan Zidni(4)

(1) Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jatinangor, Sumedang, West Java, Indonesia - Functional Nano Powder University Center of Excellence (FiNder U CoE), Universitas Padjadjaran, Jatinangor, Sumedang, West Java, Indonesia
(2) Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jatinangor, Sumedang, West Java, Indonesia
(3) Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jatinangor, Sumedang, West Java, Indonesia
(4) Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jatinangor, Sumedang, West Java, Indonesia
(*) Corresponding Author

Abstract


Cambarellus patzcuarensis is a freshwater ornamental crayfish with stable export demand, yet its culture performance is frequently constrained by the limited availability of feeds that resemble its natural trophic niche. This study investigated the potential of biofloc derived from red Nile tilapia (Oreochromis niloticus) culture as a functional natural feed supplement for C. patzcuarensis. A completely randomized design was employed with four treatments: Frozen biofloc biomass (FBB) supplementation at 10%, 15%, and 20% of biomass larva day⁻¹, and a control without FBB, each with three replicates. The plankton abundance in the biofloc suspension in this study was 418,000 ind/mL, which was dominated by the classes Chlorophyceae and Bacillariophyceae, with rotifers as the main zooplankton component. Proximate analysis showed that FBB had high nutritional potential, characterized by high moisture content (91.20%), crude protein (29.10% dw), crude lipid (3.04% dw), crude fiber (0.81% dw), ash (3.65% dw), and carbohydrates (63.40% dw). Water quality throughout the experimental period remained within optimal ranges for C. patzcuarensis rearing. Furthermore, the combined application of commercial feed at a 4% feeding rate with 15% FBB supplementation resulted in relatively improved culture performance, as reflected by a survival rate of 65.0 ± 7.51%, a specific growth rate of 3.59 ± 0.16% g day⁻¹, and a coefficient of variation of 13.31 ± 2.96%. These findings demonstrate that FBB can function as an effective feed supplement from the utilization of aquaculture effluent of the biofloc system, supporting growth performance and culture efficiency of C. patzcuarensis when applied at moderate supplementation levels.


Keywords


Biofloc biomass; C. patzcuarensis; production performance; water quality

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References

Alcorlo, P., W. Geiger & M. Otero. 2004. Feeding preferences and food selection of the Red Swamp Crayfish, Procambarus clarkii, in habitats differing in food item diversity. Crustaceana. 77: 435-453. https://doi.org/10.1163/1568540041643283

American Public Health Association (APHA). 2005. Standard Methods for the Examination of Water and Wastewater. 21st Edition, American Public Health Association/American Water Works Association/Water Environment Federation, Washington DC.

Araneda, M.E., J.M. Hernández, E. Gasca-Leyva & M.A. Vela. 2013. Growth modelling including size heterogeneity: Application to the intensive culture of White Shrimp (Penaeus vannamei) in freshwater. Aquacultural Engineering. 56: 1-12. https://doi.org/10.1016/j.aquaeng.2013.03.003

Avnimelech, Y. 2009. Biofloc technology, A practical guide book. The World Aquaculture Society. 182. https://www.cabidigitallibrary.org/doi/full/10.5555/20113266301

Avnimelech, Y., dan Kochba, M. 2009. Evaluation of nitrogen uptake and excretion by Tilapia in biofloc tanks, using 15 N tracing. Aquaculture. 287 (1): 163-168. https://doi.org/10.1016/j.aquaculture.2008.10.009

Azim, M.E & D.C. Little. 2008. The biofloc technology (BFT) in indoor tanks: Water quality, bioflocs composition and growth and welfare of Nile Tilapia (Oreochromis niloticus). Aquaculture: 29-35. https://doi.org/10.1016/j.aquaculture.2008.06.036

Barzamini, M., M. Harsij, H. Adineh & H. Jafaryan. 2021. The effect biofloc-supplemented diets on the Pacific Shrimp (Litopenaeus vannamei): Analysis of water quality growth performance, and biochemical composition. Iranian Journal of Aquatic Animal Health. 7 (2): 30-43.

Becerril-Cortes, D., M. Monroy-Dosta, M. Emerenciano, G. CastroMejıa, B. Sofia & S. Bermudez. 2018. Effect on nutritional composition of produced bioflocs with different carbon sources (Molasses, coffee waste and rice bran) in biofloc system. Int. J. Fish. Aquat. Stud. 6: 541-547. https://www.fisheriesjournal.com/archives/2018/vol6issue2/PartG/6-2-30-391.pdf

Bellinger, E.G. 1992. A key to common algae: Freshwater, estuarine and some coastal species. London: The Institute of Water and Environmental Management. https://openlibrary.org/books/OL19476027M/A_key_to_common_algae

Bernardi, F., I.V. Zadinelo, H.J. Alves, F. Meurer & L.D. Santos. 2018. Chitins & chitosans for the removal of total ammonia of aquaculture effluents. Aquaculture. 483: 203-212. https://doi.org/10.1016/j.aquaculture.2017.10.027

Boyd, C.E & C.S. Tucker. 1998. Pond Aquaculture Water Quality Management. Springer-Verlag.

Boyd, C.E. 2020. Water quality: An introduction (3rd ed.). Springer International Publishing. https://doi.org/10.1007/978-3-030-23335-8

Browdy, C.L., A.J. Ray, J.W. Leffler & Y. Avnimelech. 2012. Biofloc-based aquaculture systems. In Aquaculture Production Systems, 1st ed.;James, T.C., Ed.; John Wiley and Sons, Inc.: Hoboken, NJ, USA. 278-307. https://doi.org/10.13140/2.1.1691.4565

Camargo, J.A., A. Alonso & A. Salamanca. 2005. Nitrate toxicity to aquatic animals: A review with new data for freshwater invertebrates. Chemosphere. 58 (9): 1255-1267. https://doi.org/10.1016/j.chemosphere.2004.10.044

Crab, R., T. Defoirdt, P. Bossier & W. Verstraete. 2012. Biofloc technology in aquaculture: Beneficial effects and future challenges. Aquaculture. 356-357: 351-356. https://doi.org/10.1016/j.aquaculture.2012.04.046.

Crab, R., Y. Avnimelech, T. Defoirdt, P. Bossier & W. Verstraete. 2007. Nitrogen removal techniques in aquaculture for a sustainable production. Aquaculture. 270 (1-4): 1-14. https://doi.org/10.1016/j.aquaculture.2007.05.006

De Schryver, P., R. Crab, T. Defroit, N. Boon & V. Verstreate. 2008. The basics of bioflocs technology: The added value for aquaculture. Aquaculture. 277 (3): 125-137. https://doi.org/10.1016/j.aquaculture.2008.02.019

Durborow, R.M., D.M. Crosby & M.W. Brunson. 1997. Ammonia in fish ponds. Southern Regional Aquaculture Center (SRAC) Publication No. 463, Stoneville, Mississippi, USA, 4 pp.

Ekasari, J. 2009. Biofloc technology: Theory and applications in intensive systems aquaculture fisheries. Indonesian Journal of Aquaculture. 8 (2): 117-126. https://doi.org/10.19027/jai.8.117-126

Ekasari, J., D. Angela, S.H. Waluyo, T. Bachtiar, E.H. Surawidjaja, P. Bossier & P. De Schryver. 2014. The size of biofloc determines the nutritional composition and the nitrogen recovery by aquaculture animals. Aquaculture. 426: 105-111. https://doi.org/10.1016/j.aquaculture.2014.01.023

Emerenciano, M.G., G. Gaxiola & G. Cuzon. 2013. Biofloc technology (BFT): A review for aquaculture application and animal food industry. InTech. 301-313. http://dx.doi.org/10.5772/53902

Ercoli, F., D. Ghia & L. Gruppuso. 2021. Diet and trophic niche of the invasive signal crayfish in the first invaded Italian stream ecosystem. Sci Rep. 11: 8704. https://doi.org/10.1038/s41598-021-88073-2

Erlangga, E. 2012. Intensive cultivation of vannamei shrimp. Agromandir Library. South Tangerang.

Fahrudin, A.M., S. Subandiyono & D. Chilmawati. 2023. Pengaruh protein dalam pakan terhadap efisiensi pemanfataan pakan dan pertumbuhan juvenil Vaname (Litopenaeus vannamei). Jurnal Sains Akuakultur Tropis. 1: 114-126. https://doi.org/10.14710/sat.v7i1.17284

Fendjalang, S.N.M., T. Budiardi, E. Supriyono & I. Effendi. 2016. Production of Vannamei Shrimp Litopenaeus vannamei in karammba floating nets with different stocking density in the Strait of the Thousand Islands. Journal of Tropical Marine Science and Technology. Cell. 8 (1): 201-214. https://journal.ipb.ac.id/index.php/jurnalikt/article/view/12718

Fikriyah, A., D. Febrianti, M.C. Undu, Y. Nurliani & A. Khumaidi. 2023. Development and growth of vannamei shrimp larvae (Litopenaeus vannamei) in two shrimp hatcheries in Situbondo: A Case Study. Journal of Fisheries. 13 (1): 123-135. https://doi.org/10.29303/jp.v13i1.446

Gamberoni, P., J. Tering & M.J. Slater. 2025. Effects of chronic nitrate stress on Litopenaeus vannamei reared in indoor aquaculture systems. Aquaculture International. 33: 714. https://doi.org/10.1007/s10499-025-02379

García-Ballesteros, S., B. Villanueva & J. Fernández. 2021. Genetic parameters for uniformity of harvest weight in Pacific White Shrimp (Litopenaeus vannamei). Genet Sel Evol. 53: 26. https://doi.org/10.1186/s12711-021-00621-6

Goddard, S. 1996. Feed management in intensive aquaculture. In Feed Management in Intensive Aquaculture. Springer US. https://doi.org/10.1007/978-1-4613-1173-7

Hargreaves, J.A. 2013. Biofloc production systems for aquaculture. SRAC Publication. 4503. Southern Regional Aquaculture Center. Retrieved from https://srac.tamu.edu/

Helal, A.M., M.M. Zaher, D.T. Meshhal, M. Ashour, E.M. Younis, A.A. Abdelwarith, A.D.G. Al-Afify, Z.Z. Sharawy, S. Davies, E. El-Haroun & M.G. Nassif. 2024. Biofloc supplementation improves growth performances, nutrient utilization, and histological status of Nile Tilapia (Oreochromis niloticus) while enhancing zooplankton diversity, community, and abundance. Aquaculture, 585. 740711. https://doi.org/10.1016/j.aquaculture.2024.740711

Holdich, D.M. 2002. Biology of Freshwater Crayfish. Oxford, UK: Blackwell Science Ltd. ISBN: 978-0-632-05411-8. https://doi.org/10.1651/0278-0372(2002)022[0969:BOFC]2.0.CO;2

Huang, Y., P. Gao, D. Yu, Z. Sun, X. Yang, Q. Lai & H. Chi. 2025. A comparative analysis on the biochemical composition and nutrition evaluation of crayfish (Procambarus clarkii) cultivated in saline-alkali and fresh water. Foods. 14 (11): 1997. https://doi.org/10.3390/foods14111997

Infofish International. 2024. The importance of monitoring shrimp uniformity for better performance in the production cycle. Infofish International Magazine. 1: 45-50.

Irianti, D.S.A., Y. Yustiati & H.I. Hamdani. 2016. Kelangsungan hidup dan pertumbuhan udang galah (Macrobrachium rosenbergii) yang diberi kentang pada media pemeliharaan. Jurnal Perikanan Kelautan. 6 (2): 92-97. https://jurnal.unpad.ac.id/jpk/article/view/13932

Kuhn, D.D., G.D. Boardman, A.L. Lawrence, L. Marsh & G.J. Flick. 2009. Microbial floc meal as a replacement ingredient for fish meal and soybean protein in shrimp feed. Aquaculture. 296: 51-57. https://doi.org/10.1016/j.aquaculture.2009.07.025

Kumar, V., S. Roy, B.K. Behera, H.S. Swain & B.K. Das. 2021. Biofloc microbiome with bioremediation and health benefits. Front. Microbiol. 12: 741164. https://doi.org/10.3389/fmicb.2021.741164

Lante, S., U. Usman & A. Laining. 2015. Pengaruh kadar protein pakan terhadap pertumbuhan udang windu, Penaeus monodon Fab. Transveksi. Jurnal Perikanan Universitas Gadjah Mada. 17 (1): 10-17. https://journal.ugm.ac.id/jfs/article/view/9936/7470

Lin, W., H. Luo, J. Wu T.-C. Hung, B. Cao, X. Liu, J. Yang & P. Yang. 2023. A review of the emerging risks of acute ammonia nitrogen toxicity to aquatic decapod crustaceans. Water. 15 (1): 27. https://doi.org/10.3390/w15010027

Liu, X., Y. Wang, H. Liu, Y. Zhang, Q. Zhou, X. Wen, W. Guo & Z. Zhang. 2024. A systematic review on aquaculture wastewater: Pollutants, impacts, and treatment technology. Environmental Research. 262 (1): 119793. https://doi.org/10.1016/j.envres.2024.119793

Lunda, R., K. Roy, P. Dvorak, A. Kouba & J. Mraz. 2020. Recycling biofloc waste as a novel protein source for crayfish with special reference to crayfish nutritional standards and growth trajectory. Scientific Reports. 10: 19607. https://doi.org/10.1038/s41598-020-76692-0

Monroy-Dosta, M.D.C., R. De Lara-Andrade, J. Castro-Mejía, G. Castro-Mejía & M.G. Coelho-Emerenciano. 2013. Composición y abundancia de comunidades microbianas asociadas al biofloc en un cultivo de tilapia. Revista de biología marina y oceanografía. 48 (3): 511-520. http://dx.doi.org/10.4067/S0718-19572013000300009

Munguti, J., J.G. Kirimi, K. Obiero & L. Musali. 2020. Aqua-feed wastes: Impact on natural systems and practical mitigations - A review. Journal of Agricultural Science. 13 (1): 111. https://doi.org/10.5539/jas.v13n1p111

Nisar, U., D. Peng, Y. Mu & Y. Sun. 2022. A solution for sustainable utilization of aquaculture waste: A comprehensive review of biofloc technology and aquamimicry. Front. Nutr. 8: 791738. https://doi.org/10.3389/fnut.2021.791738

O’Hea Miller, S.B., M.Y.L. Wong, D. Mazumder, R. Gray & A.R. Davis. 2023. Will the experimental population control of an invasive crayfish influence the diet and trophic position of a native crayfish? An assessment using stable isotopes. Marine & Freshwater Research. 74: 1536-1550. https://doi.org/10.1071/MF23157

Pantjara, B., A. Nawang, U. Usman & R. Rachmansyah. 2012. Pemanfaatan bioflok pada budidaya udang vaname (Litopenaeus Vannamei) intensif. J. Ris. Akuakultur. 7 (1): 61-72. http://dx.doi.org/10.15578/jra.7.1.2012.61-72

Prescott GW. 1978. How to know the freshwater algae. Iowa: Wm C. Brown Company

Rahman, M.H., M.M. Haque, M.A. Alam & F. Flura. 2022. A study on the specific growth rate (SGR) at different stages of Tilapia (Oreochromis niloticus) production cycle in tank-based aquaculture system. International Journal of Aquaculture and Fishery Sciences. 8 (2): 59-65. https://doi.org/10.17352/2455-8400.000079

Rahmawati, A.I., R.N. Saputra, A. Hidayatullah, A. Dwiarto, H. Junaedi, D. Cahyadi, H.K.H. Saputra, W.T. Prabowo, U.K.A. Kartamiharja, H. Shafira, A. Noviyanto & N.T. Rochman. 2021. Enhancement of Penaeus vannamei shrimp growth using nanobubble in indoor raceway pond. Aquaculture and Fisheries. 6 (3): 277–282. https://doi.org/10.1016/j.aaf.2020.03.005

Ricker, W.E. 1975. Computation and interpretation of biological statistics of fish population. Bull. Fish. Res. Board Can. 191: 1-382. https://publications.gc.ca/site/eng/9.581563/publication.html

Saeedi, K.H & M. Chapara. 2024. Isolation, identification, and biofloc production: Potential of floc-forming bacteria using a novel monoculture approach and medium. Aquaculture Studies. 24 (4). https://doi.org/10.4194/AQUAST1878

Schveitzer, R., R. Arantes, P. Costodio, C. Santo, S.W. Arana & E. Andreatta. 2012. Effect of different biofloc levels on microbial activity, water quality and performance of Litopenaeus vannamei in a tank system operated with no water exchange. Aquac. Eng. 56: 59-70. https://doi.org/10.1016/j.aquaeng.2013.04.006

Shahkar, E.H., G. Yun, I.K. Park, S.K. Jang, K. Kim, K. Katya & S.C. Bai. 2014. Evaluation of optimum dietary protein level for juvenile Whiteleg Shrimp (Litapenaeus vannamei). Journal of Crustacean Biology. 34 (5): 522-558. https://doi.org/10.1163/1937240X-00002267

Supono, S., R.T. Pinem & E. Harpeni. 2021. Performa udang vaname Litopenaeus vannamei (Boone, 1931) yang dipelihara pada sistem biofloc dengan sumber karbon berbeda. Jurnal Kelautan. 24 (2). https://doi.org/10.21107/jk.v14i2.9191

Valencia-Castañeda, G., M.G. Frías-Espericueta, R.C. Vanegas-Pérez, J.A. Pérez-Ramírez, M.C. Chávez-Sánchez & F. Páez-Osuna. 2018. Acute toxicity of ammonia, nitrite, and nitrate to shrimp Litopenaeus vannamei postlarvae in low-salinity water. Bulletin of Environmental Contamination and Toxicology. 101 (2): 229-234. https://doi.org/10.1007/s00128-018-2355-z

Yang, X., Z. Tang, K. Huang, R. Guo, D. Wang, S. Jiang & K. Yu. 2025. Optimal feeding levels to enhance growth performance and gut microbiota balance in Red Swamp Crayfish (Procambarus clarkii). Aquaculture Reports. 41: 102717. https://doi.org/10.1016/j.aqrep.2025.102717

Yu, Q., J. Xie, M. Huang, C. Chen, D. Qian, J.G. Qin, L. Chen, Y. Jia & E. Li. 2020. Growth and health responses to long-term pH stress in Pacific White Shrimp Litopenaeus vannamei. Aquaculture Reports. 16: 100280. https://doi.org/10.1016/j.aqrep.2020.100280

Yulisa, V.A., A.A. Handaka, U. Subhan & A. Nurhayati. 2025. Analisis hubungan karakteristik sosial ekonomi pembudidaya terhadap produktivitas budidaya ikan hias di Kota Cimahi. Jurnal Galung Tropika. 14 (1): 113-122. https://doi.org/10.31850/jgt.v14i1.1247

Zaidy, A.B., A.D. Anggoro & A. Kasmawijaya. 2021. Effect of the use of nanobubbles in the transport of vannamei shrimp (Litapenaeus vannamei). Indonesian Aquatics. 6 (2): 50-56. https://jurnal.unpad.ac.id/akuatika-indonesia/article/view/35723/17133

Zhang, J.-X., M.-R. Li, C. Liu, S.-P. Wang & Z.-G. Yan. 2023. A review of the toxic effects of ammonia on invertebrates in aquatic environments. Environmental Pollution. 336: 122374.https://doi.org/10.1016/j.envpol.2023.122374

Zulfahmi, I., M. Syahimi & M. Muliari. 2018. Effect of adding biofloc with different doses on the growth of windu shrimp (Penaeus monodon Fabricius 1798). Al-Kauniyah: Journal of Biology. 11 (1): 1-8. https://doi.org/10.15408/kauniyah.v11i1.4862

How to Cite this Article:

Subhan, U., S.A. Kamilah, L.P. Dewanti & I. Zidni­. 2026. Frozen biofloc biomass as a functional feed supplement enhances production performance of Mexican Dwarf Crayfish (Cambarellus patzcuarensis). Jurnal Perikanan Universitas Gadjah Mada. 28 (1): 61-70. https://doi.org/10.22146/jfs.115523



DOI: https://doi.org/10.22146/jfs.115523

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