Pengaruh propolis terhadap profil lipid plasma tikus model hiperkolesterolemia
Krisnansari Diah(1*), Ariadne Tiara Hapsari(2), Evy Sulistyoningrum(3), Agus Prastowo(4)
(1) Jurusan Kedokteran Fakultas Kedokteran dan Ilmu-Ilmu Kesehatan, Universitas Jenderal Soedirman
(2) Jurusan Kedokteran Fakultas Kedokteran dan Ilmu-Ilmu Kesehatan, Universitas Jenderal Soedirman
(3) Jurusan Kedokteran Fakultas Kedokteran dan Ilmu-Ilmu Kesehatan, Universitas Jenderal Soedirman
(4) Instalasi Gizi Rumah Sakit Prof. Dr. Margono Soekarjo
(*) Corresponding Author
Abstract
Background: Nowadays, cardiovascular disease caused by hypercholesterolemia has become the main cause of death. Propolis has been used widely to reduce plasma cholesterol levels.
Objective: The aims of this research was to study the effect of propolis on lipid profile of hypercholesterolemic Sprague Dawley rats.
Method: This was an experimental study with pre-post test. Twenty four (24) male Sprague Dawley rats aged 12-16 week old, weighing 125-200 g were allocated into 4 groups. Group I received standard meal + aquadest-gavage; group II received high cholesterol meal + PTU 0,01 + aquadest gavage; group III received high cholesterol meal + PTU 0,01 + 0,027 g propolis gavage; group IV received high cholesterol meal + PTU 0,01 + 0,054 g propolis gavage. Total cholesterol, triglycerides, HDL cholesterol and LDL cholesterol levels before and after treatment were measured. The data were then analyzed with One Way Anova.
Results: The study showed that there were no significant differences in changes of body weight. There were significant differences in total cholesterol levels between all groups of treatment. Triglyceride levels were significantly different among all groups, except between group I and IV. Furthermore, the HDL cholesterol levels of group I vs III and group I vs IV were significantly different. However, there were no differences found in LDL cholesterol levels among all groups of treatment.
Conclusion: Provision of 0,027 g and 0,054 g propolis improve lipid profile (total cholesterol, triglyceride and HDL cholesterol levels) of hypercholesterolemic rats.
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Purwaningsih E, Sugiri. Gizi dan kesehatan jantung. Harapan baru pencegahan dan pengelolaan penyakit jantung iskemik. Semarang: Badan Penerbit Universitas Diponegoro Semarang; 2008.
Riset Kesehatan Dasar. Laporan hasil riset kesehatan dasar [serial online] 2007 [cited 2012 Jan 2]. Available from: http://www.docstoc.com/Laporan-Hasil-Riset-Kesehatan-Dasar (Riskes).
Guyton AC, Hall JE. Buku ajar fisiologi kedokteran. Jakarta: Penerbit Buku Kedokteran EGC; 1997.
Betteridge DJ. Lipid in current perspective. United Kingdom: Martin Duniltz Ltd; 1996.
Anwar TB. Dislipidemia sebagai faktor resiko penyakit jantung koroner. Sumatera Utara: Fakultas Kedokteran Universitas Sumatera Utara; 2008.
Rainwater DL, Mahaney MC, Vandeberg JL, Wang XL. Vitamin E dietary supplementation significantly affects multiple risk factors for cardiovascular disease in baboons. Am J Clin Nutr 2007;86:597-603.
Ganiswara SG. Farmakologi dan terapi. Jakarta: Gaya Baru; 2003.
Limantara, Leenawati, Rahayu, Puji. Studi lapangan kandungan klorofil in vivo beberapa tumbuhan hijau di Salatiga dan sekitarnya. Jakarta: Seminar MIPA; 2005.
Jamal S, Suhardi. Penggunaan obat tradisional oleh anggota rumah tangga Jawa dan Bali menurut SKRT 1995. Media Litbangkes 1999;VII(3,4):10-3.
Anonim-1. Propolis diamond (bee pollen/royal jelly/propolis) [serial online] 2009 [cited 2010 Feb 4]. Available from: www.dutaherbal.com.
El-Sayed M El-Sayed, Abo-Salem MA, A Aly H, Mansour MA. Potential antidiabetic and hypolipidemic effects of propolis extract in streptozotocin-induced diabetic rats. Pak J Pharm Sci 2009;22(2):168-74.
Myung-Sang K, Zhong-Zhe H, Soo-Yeon P, Hyoung-Chun K, Sang-Duk K. Effects of propolis on the accumulation cholesterol induced by high-cholesterol diet in rats [serial online] 2009 [cited 2010 July 15]. Available from: www.apinetla.com.ar/congreso/15.pdf
Linawati M, Bagiada M. Pengaruh propolis terhadap sekresi interleukin-12 pada supernatan kultur makrofag dari penderita tuberkulosis paru yang diinfeksi Mycobacterium tuberculosis [serial online] 2009 [cited 2010 June 5]. Available from: ejournal.unud.ac.id.
Seven PT, Yilmaz S, Seven I, Cerci IH, A Azman M, Yilmaz M. Effects of propolis on selected blood indicators and antioxidant enzyme activities in broiler unders heat stress. ACTA VET. BRNO 2009;78:75–83.
Lotfy M. Biological activity of bee propolis in health and disease. Asian Pac J Cancer Prev 2006;7(1):22-31.
Geckil H, Ates B, Durmaz G, Erdogan S, Yilmaz I. Antioxsidant, free radical scavenging and metal chelating characteristics of propolis. Am J Biochem Biotechnol 2005;1(1):27-31.
Ramos AFN, Miranda JL. Propolis: a review of its anti-inflamatory and healing action. J Venom Anim Toxins Inci Trop Disc 2007;13(4):697-710.
Kusumawati D. Bersahabat dengan hewan coba. Yogyakarta: Gadjah Mada University Press; 2004.
Momuat LI. Minyak sawit mempercepat regresi aterosklerosis aorta pada kelinci hiperlipidemia ringan tetapi tidak pada hiperlipidemia berat. Prosiding Seminar Nasional Tanaman Obat dan Obat Tradisional; 2007 Juli 10-11; Surakarta.
Elizabeth BEK. Ekstrak daun jati belanda (Guazuma ulmifolia Lakl) penurun kolesterol tanpa mengganggu fungsi hati. Prosiding Seminar Nasional Tanaman Obat dan Obat Tradisional; 2007 Juli 10-11; Surakarta.
Dahlan MS. Statistik untuk kedokteran dan kesehatan. Jakarta: Salemba Medika; 2008.
(KKI) Kelompok Kerja Ilmiah Phyto Medica. Pedoman pengujian dan pengembangan fitofarmaka. Jakarta: Yayasan Pengembangan Obat Bahan Alam Phyto Medica; 1993.
Chambers CM, Ness GC. Dietary cholesterol regulates hepatic 3-hydroxy-3-methylglutaryl coenzyme a reductase gene expression in rats primarily at the level of translation. Arch Biochem Byophys 1998;354(2):317-22.
Murrai RK. Harper’s biochemistry. Alexander HS, editor. Andry H (Alih bahasa). Jakarta: Penerbit Buku Kedokteran; 1999.
Marks DB, Marks AD, Smith CM. Biokimia kedokteran dasar, sebuah pendekatan klinis. Brahm U Pendit (Alih bahasa). Jakarta: EGC; 2000.
Grundy SM. Multifactorial etiology of hypercholesterolemia: implication of prevention coronary heart disease (review). Artherioscler Thromb 1991;11(6):1619-35.
Ristanti ET, Lestariana W, Lestari LA. Swamedikasi diabetes mellitus dengan daun ceplikan (Ruellia tuberosa L): kajian kemanfaatan pada profil lipid serum secara praklinik. Jurnal Gizi Klinik Indonesia 2009;5(3):128-32.
Rukmasari EA, Hadi H, Achadiono DNW. Pengaruh suplementasi vitamin C dan vitamin E terhadap profil lipid pada pasien penyakit jantung koroner di Poliklinik Kardiologi RSU dr.Slamet Garut. Sains Kesehatan 2006;19(2).
DOI: https://doi.org/10.22146/ijcn.17600
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