Fortifikasi besi dan prebiotik pada kukis terhadap histologi usus tikus anemia

https://doi.org/10.22146/ijcn.42606

Muhana Rafika(1), Lily Arsanti Lestari(2), Siti Helmyati(3*)

(1) Departeman Biostatistik, Epidemiologi, dan Kesehatan Populasi, Fakultas Kedokteran, Kesehatan Masyarakat, dan Keperawatan Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Departemen Gizi Kesehatan, Fakultas Kedokteran, Kesehatan Masyarakat, dan Keperawatan Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Departemen Gizi Kesehatan, Fakultas Kedokteran, Kesehatan Masyarakat, dan Keperawatan Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author

Abstract


Iron dan prebiotic fortification in cookies towards intestine histology anemia rats

Background: Anemia can also cause damage to small intestinal villous cells. Iron fortification can be an alternative prevention of anemia. Prebiotic additives can reduce the effects of fortification and improve intestinal villous.

Objective: The study aims to determine the effect of breadfruit and soybean-based cookies with prebiotic and iron fortification on the small intestine's histology, especially the intestinal villous's height and width.

Methods: Experimental study with posttest control group design with a total sample of 48 rats. Rats were divided into eight groups, given breadfruit and soybean-based cookies with the addition of FOS and NaFeEDTA (K1), FOS and FeSO4 (K2), FOS (K3), FeSO4 (K4), NaFeEDTA (K5), without fortification (K6). In addition, K- is a healthy mouse with standard feed, and K + is an anemia mouse. Anemia induction is for two weeks, and intervention is for 28 days. Intestinal histology was observed using an optilab microscope and measured using ImageRaster 4.0.5.

Results: There were no significant differences in duodenum villous height, jejunum villous height, and ileum villous width (p>0.05). However, there were significant differences (p <0.05) for duodenum villous width (K- and K3), jejunum villous width (K1 and K4) and ileum villous height (K1, K2 and K +).

Conclusions: Giving breadfruit-based and soybeans with prebiotic and iron fortification can increase the height and width of small intestinal villi.


Keywords


anemia; breadfruit; cookies; iron; prebiotic; soy; villious

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References

  1. World Health Organization (WHO). The global prevalence of anaemia in 2011. [series online] 2015 Available from: URL: https://www.who.int/publications/i/item/9789241564960
  2. Kemenkes RI. Riset kesehatan dasar 2013. Jakarta: Kementerian Kesehatan RI; 2013.
  3. Citrakesumasari. Anemia gizi, masalah dan pencegahannya. Yogyakarta: Kalika; 2012.
  4. Permaesih D, Herman S. Faktor-faktor yang mempengaruhi anemia pada remaja. Buletin Penelitian Kesehatan. 2005;33(4):162–71.
  5. Guha DK, Walia BNS, Tandon BN, Deo MG, Ghai OP. Small bowel changes in iron-deficiency anaemia of childhood. Arch Dis Childh. 1968;43(228):239–44. doi: 10.1136/adc.43.228.239
  6. Gulec S, Anderson GJ, Collins JF. Mechanistic and regulatory aspects of intestinal iron absorption. Am J Physiol Gastrointest Liver Physiol. 2014; 307(4):G397–409. doi: 10.1152/ajpgi.00348.2013
  7. Li Y. Dietary iron deficiency and oversupplementation increase intestinal permeability, ion transport, and inflammation in pigs. J Nutr 2016;146(8):1499–505. doi: 10.3945/jn.116.231621
  8. Soekirman, Martianto D, Airesa K. Fortifikasi pangan strategi global penanggulangan kurang zat gizi mikro. Jakarta: Yayasan Kegizian untuk Pengembangan Fortifikasi Pangan Indonesia; 2015.
  9. Muryanti S. Kue kering. Yogyakarta: Venus; 2009.
  10. Ferawati, Muchtadi D. Formulasi dan pembuatan banana bars berbahan dasar tepung kedelai, terigu, singkong dan pisang sebagai alternatif pangan darurat. J Teknol Pangan. 2009;2(1):67–78.
  11. Cheung YB, Xu Y, Mangani C, Fan Y, Dewey KG, Salminen SJ, et al. Gut microbiota in Malawian infants in a nutritional supplementation trial. Trop Med Int Heal. 2016;21(2):283–90. doi: 10.1111/tmi.12650
  12. Waryat M, Handayani Y. Diversifikasi pangan dari tepung sukun untuk mengurangi konsumsi tepung terigu di Kepulauan Seribu, Provinsi DKI Jakarta. Buletin Pertanian Perkotaan. 2014;4(1):13–9.
  13. Nuraida L, Palupi NS, Putri DE, Widayanti NWY. Potensi talas (Colocasia esculenta ( l ) schott) dan sukun (Artocarpus altilis (park) fosberg) untuk mendukung pertumbuhan bakteri asam laktat probiotik. In: Prosiding Mikrobiologi dan Bioteknologi. Yogya; 2006.
  14. Warchol M, Perrin S, Grill J, Schneider F. Characterization of a purified b -fructofuranosidase from Bifidobacterium infantis ATCC 15697. Lett Appl Microbiol. 2002;35:462–7.
  15. Hurrell RF. Fortification: overcoming technical and practical barriers. J Nutr. 2002;132(4 Suppl):806S–12S. doi: 10.1093/jn/132.4.806S
  16. Kandarina I, Helmyati S, Lily Arsanti L. Efektifitas pemberian biskuit tepung singkong yang difortifikasi zat besi pada anak usia sekolah dasar di Kabupaten Bantul: upaya mengatasi defisiensi gizi mikro berbasis pangan lokal. [Laporan Laporan Akhir Hibah Penelitian Strategis Nasional]. Yogyakarta: Universitas Gadjah Mada; 2010.
  17. Buhnik-Rosenblau K, Moshe-Belizowski S, Danin-Poleg Y, Meyron-Holtz EG. Genetic modification of iron metabolism in mice affects the gut microbiota. Biometals. 2012;25(5):883–92. doi: 10.1007/s10534-012-9555-5
  18. Allen L, Benoist B De, Dary O, Hurrell R. Guidelines on food fortification with micronutrients. [series online] 2006 Available from: URL: https://www.who.int/publications/i/item/9241594012
  19. Nurdiyawati E, Helmyati S. Pengaruh fortifikasi besi terhadap sifat antibakteri susu fermentasi sinbiotik [Skripsi]. Yogyakarta: Universitas Gadjah Mada; 2013.
  20. Nurhamidah E. Pengaruh pemberian ekstrak ubi jalar ungu (Ipomoea batatas poiret) terhadap kadar glukosa darah, kadar immunoglobulin A (IgA), dan villi usus pada tikus putih jantan (Rattus norvegitus) diabetes melitus. Scientia Jurnal Farmasi dan Kesehatan. 2014;4(1):22–8.
  21. Islamiah AR. Uji toksisitas akut gelatin babi pada tikus betina galur Sprague dawley [Skripsi]. Jakarta: UIN Syarif Hidayatullah; 2016.
  22. Van Putten LM. The life span of red cells in the rat and the mouse as determined by labeling with DFP32 in vivo. Blood.1958;13(8):789-794.
  23. Sunarno, Goeltoem RJ, Mardiati SM. Aplikasi pakan kaya nutrisi dengan suplementasi daging ikan gabus (Channa striata) dan perannya dalam perbaikan struktur duodenum: kajian in vivo pada tikus wistar yang diberi perlakuan stres. Bioma Jurnal Ilmiah Biologi. 2016;5(1):43-60. doi: 10.26877/bioma.v5i1.1493
  24. Gomez-Alcon RA, Dudas C, Huber JT. Influence of cultures of Aspergillus oryzae on rumen and total tract digestibility of dietary components. J Dairy Sci. 1990;73(3):703-10. doi: 10.3168/jds.S0022-0302(90)78723-1
  25. Roberfroid MB. Inulin-type fructans: functional food ingredients. J Nutr. 2007;137(11 Suppl):2493S-2502S. doi: 10.1093/jn/137.11.2493S
  26. Gunal M, Yayli G, Kaya O, Karahan N, Sulak O. The effects of antibiotic growth promoter, probiotic or organic acid supplementation on performance, intestinal microflora and tissue of broilers. International Journal of Poultry Science.2006;5(2):149-55. doi: 10.3923/ijps.2006.149.155
  27. Dellman HD, Brown E. Histologi veteriner ed. III. Jakarta: UI Press; 1992.
  28. Fanani AF, Shutama N, Sukamto B. Retensi nitrogen dan konversi pakan ayam lokal persilangan yang diberi ekstrak umbi dahlia (dahlia variabilis) sebagai sumber inulin. Jurnal Sains Peternakan. 2014;12(2):35–7. doi: 10.20961/sainspet.v12i2.4762
  29. Widodo TS, Sulistiyanto B, Utama CS. Jumlah bakteri asam laktat BAL) dalam digesta usus halus dan sekum ayam broiler yang diberi pakan ceceran pabrik pakan yang difermentasi. Jurnal Agripet. 2015;15(2):98–103. doi: 10.17969/agripet.v15i2.2376
  30. Rosa NR. Pengaruh penambahan umbi garut (Maranta arundinaceae L) dalam bentuk tepung dan pati sebagai prebiotik pada yoghurt sebagai produk sinbiotik terhadap daya hambat bakteri Escherichia Coli [Disertasi]. Semarang: Universitas Diponergoro; 2010.
  31. Astuti TYI, Ekawati LM, Purwijantiningsih, Pranata S. Subtitusi tepung sukun dalam pembuatan non flaky crakers bayam hijau (Amaranthus tricolor). Jurnal Agros. 2013;1–13.
  32. Giannenas I, Papaneophytou CP, Tsalie E, Pappas I, Triantafillou E, Kontopidis GA, et al. Dietary supplementation of benzoic acid and essential oil compounds affects buffering capacity of the feeds, performance of turkey poults and their antioxidant status, pH in the digestive tract, intestinal microbiota and morphology. Asian-Australas J Anim Sci. 2014;27(2):225-36. doi: 10.5713/ajas.2013.13376
  33. Samanta S, Haldar S, Ghosh TK. Comparative efficacy of an organic Acid blend and bacitracin methylene disalicylate as growth promoters in broiler chickens: effects on performance, gut histology, and small intestinal milieu. Vet Med Int. 2010;2010:645150. doi: 10.4061/2010/645150
  34. Miles RD, Butcher GD, Henry PR, Littell RC. Effect of antibiotic growth promoters on broiler performance, intestinal growth parameters, and quantitative morphology. Poult Sci. 2006;85(3):476-85. doi: 10.1093/ps/85.3.476
  35. Zhang J, Liu C, Xie Y, Li N, Ning Z, Zhong Y, et al. Enhancing fructooligosaccharides production by genetic improvement of the industrial fungus Aspergillus niger ATCC 20611. J Biotechnol. 2017;249:25-33. doi: 10.1016/j.jbiotec.2017.03.021
  36. Solis de los Santos F, Farnell MB, Tellez G, Balog JM, Anthony NB, Donoghue AM, et al. Effect of prebiotik on gut development and ascites incidence of broilers reared in a hypoxic environment. Poultry Sci. 2005;84(7):1092-100. doi: 10.1093/ps/84.7.1092
  37. Mamiek B. Fermacto versus enzymes. USA: Fermacto. Pet. Ag. Inc. ed., Elgin, IL; 1993.



DOI: https://doi.org/10.22146/ijcn.42606

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