Seleksi Isolat Bakteri Amilolitik dari Rhizosfer Canna edulis, Kerr. untuk Produksi Poli Hidroksi Alkanoat dari Limbah Cair Tapioka

https://doi.org/10.22146/jrekpros.33194

Nurhayati Nurhayati(1*), Ocky Karna Radjasa(2), Irfan Dwidya Prijambada(3)

(1) Departemen Biologi Fakultas Sains dan Matematika Universitas Diponegoro
(2) Departemen Perikanan dan Kelautan Universitas Diponegoro
(3) Lab. Mikrobiologi Tanah dan Lingkungan Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Petrochemical-based plastic waste accumulated in landfills have been posing serious threat to the environment as this kind of plastics are non-biodegradable. Replacing petrochemical-based plastics with biodegradable plastics constitutes a challenging solution both in terms of mechanical design of the process and most importantly the availability of powerful local microorganism for the process. Therefore, the current study was searching for appropriate local microorganisms for poly hydroxyl alkanoate (PHA) production from starch waste, which was considered as one of cheap carbon sources. Waste water of cassava industry is a good resource of such starch waste water. The microbes were isolated from Canna edulis, Kerr. rhizosphere from Cangkringan.  The expected isolates were the bacteria enable the coupling of carbon catabolic pathways with PHA anabolic pathways. It was found that ten isolates were able to use waste water of cassava flour industry as carbon source. The PHA quantitative analysis by spectrophotometer showed that the isolate of Bacillus sp. C8 produced the highest PHA of 2,095 g/L. Further FTIR analysis showed specific bands near 1363,67 cm-1, 1641,42 cm-1, 2929,87 cm-1, 3408,22 cm-1 wavelengths which revealed the presence of CH3, ester carbonyl group (C=O), C-H and terminal OH group of PHA.

 

ABSTRAK

Akumulasi sampah plastik berbasis petrokimia di tempat pembuangan sampah mengganggu lingkungan karena plastik sifatnya tidak mudah didegradasi secara biologi dan sangat tahan di lingkungan. Penggantian plastik yang berasal dari bahan petrokimia dengan bahan plastik yang mudah terdegradasi secara biologi merupakan tantangan tersendiri, baik dari sisi perancangan proses maupun ketersediaan mikrobia lokal yang sesuai untuk proses tersebut. Oleh karena itu, penelitian ini dilakukan sebagai upaya untuk mendapatkan bakteri lokal penghasil PHA yang mampu mengkonsumsi substrat berupa pati. Substrat pati dipilih karena ketersediaan limbat industri tapioka sebagai bahan baku potensial dan murah untuk produksi PHA. Bakteri amilolitik untuk produksi PHA telah berhasil diisolasi dari rhizosfer Canna edulis, Kerr. di Cangkringan, Sleman, Yogyakarta, Indonesia. Bakteri yang diisolasi merupakan bakteri dengan kemampuan memproduksi PHA dan memiliki kemampuan melakukan rangkaian reaksi pada limbah cair industri tapioka dan rangkaian reaksi pembentukan PHA. Telah berhasil didapatkan 10 bakteri yang memiliki aktivitas amilolitik dan dapat menghasilkan PHA  menggunakan limbah cair industri tapioka. Analisis kuantitatif PHA menggunakan spektrofotometer menunjukkan bahwa isolat Bacillus sp. C8 menghasilkan PHA paling tinggi sebesar 2,095 mg/mL. Analisis hasil metabolism isolat C8 menggunakan FTIR memperlihatkan puncak spesifik 1363,67 cm-1, 1641,42 cm-1, 2929,87 cm-1, 3408,22 cm-1 adalah verifikasi adanya CH3, C=O, C-H dan OH dari PHA.

Keywords


Canna edulis; Kerr. rhizosphere; wastewater; assava industry flour; PHA; bakteri

Full Text:

PDF


References

Baskan K.S., Tutem E., Akyuz E., Ozen S., and Apak R., 2016, Spectrophotometric total reducing sugras assay based on cupric reduction, Tlanta, 147, 162-168.

Diez-Pascual N.H., and Diez-Vicente A.L., 2014, Poly(3-hydroxybutyrate)/ZnO bionanocomposites with improved mechanical, barrier and antibacterial properties, Int. J. Mol., 15, 10950-10973.

Gatechew A., and Woldensenbet F., 2016, Production of biodegradable plastic by polyhydroxybutyrate (PHB) accumulating bacteria using low cost agricultural waste material, BMC Res., 9, 1-9.

Giedraityfa G. and Kalediene L., 2016. Purification and characterization of polyhydroxybutyrate (PHB) produced from thermophilic geobacillus sp. AY 946034 strain. Chemija 26(1) 38-45.

Nurhayati, Prijambada I.D., Radjasa O.K., dan Widada J., 2017, Repetitive element palindromic PCR (Rep-PCR) as a genetic tool to study diversity in amylolytic bacteria, Advanced Science Letters, 23, 6458-6461.

Putra, J.A., Wiratni, Syamsiah S., and Redyowati, S., 2007, Kinetics of lysis and extraction of intracellular PHB by Cupriavidus necator (CCUG 52238 T) using differential method by H2O2-chloroform”, Seminar Nasional Teknik Kimia UNPAR, April 2007.

Vinish V., Sangetta S.H., Aravand J., Kanmani P., and Sathiskumar T., 2015, Optimizing the nutrient feeding strategy for PHA production by a novel strain of Enterobacter sp., Int. J. Environ. Sci. Technol., 12, 2757-2764.



DOI: https://doi.org/10.22146/jrekpros.33194

Article Metrics

Abstract views : 723 | views : 1353

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 Jurnal Rekayasa Proses

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Rekayasa Proses  (print ISSN 1978-287X; online ISSN 2549-1490) is published by Chemical Engineering Department, Faculty of Engineering, Universitas Gadjah Mada. View website statistics.