Escherichia coli O157:H7 Bacteria Antibiotic Resistant Isolated from Flies at Food Courts in IPB Dramaga Campus
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| 1. | Title | Title of document | Escherichia coli O157:H7 Bacteria Antibiotic Resistant Isolated from Flies at Food Courts in IPB Dramaga Campus |
| 2. | Creator | Author's name, affiliation, country | Muhammad Rizki Aminudin; Program Pascasarjana, Sekolah Kedokteran Hewan dan Biomedis, IPB University; Indonesia |
| 2. | Creator | Author's name, affiliation, country | Denny Widaya Lukman; Divisi Kesehatan Masyarakat Veteriner dan Epidemiologi, Sekolah Kedokteran Hewan dan Biomedis, IPB University; Indonesia |
| 2. | Creator | Author's name, affiliation, country | Mirnawati Bachrum Sudarwanto; Divisi Kesehatan Masyarakat Veteriner dan Epidemiologi, Sekolah Kedokteran Hewan dan Biomedis, IPB University |
| 2. | Creator | Author's name, affiliation, country | Herwin Pisestyani; Divisi Kesehatan Masyarakat Veteriner dan Epidemiologi, Sekolah Kedokteran Hewan dan Biomedis, IPB University; Indonesia |
| 3. | Subject | Discipline(s) | Antibiotic Resistance |
| 3. | Subject | Keyword(s) | Escherichia coli O157:H7; housefly; multi-drug resistance |
| 4. | Description | Abstract | Several human and animal pathogens transmit into the food chain through houseflies as mechanical vectors, one of which is E. coli O157:H7. E. coli O157:H7 can express Shiga toxin (Stx) which can cause diarrhea, hemorrhagic colitis, and potentially fatal hemolytic-uremic syndrome (HUS) in humans. Some pathogen strains show resistance against various antibiotics, causing complex health problems. This study aims to analyze the presence of antibiotic-resistant E. coli O157:H7 bacteria carried by houseflies (M. domestica) in the food court IPB Dramaga campus area. Detection of E. coli O157:H7 on fly legs using qPCR method based on MU 7.2.3.32-8. E. coli O157:H7 isolates were tested for sensitivity to the antibiotic’s ciprofloxacin, ampicillin, tetracycline, trimethoprim-sulfamethoxazole, cefepime, chloramphenicol, ceftazidime, cefotaxime, and ceftriaxone using the Kirby Bauer disc diffusion method. This study isolated 5 E. coli isolates (5/40; 12.5%), and 2 of them were confirmed as E. coli O157:H7. One isolate of E. coli O157:H7 was resistant against ampicillin and tetracycline, and one isolate was resistant against ampicillin, tetracycline, trimethoprim-sulfamethoxazole, and ceftazidime. The multi-drug resistance was identified only in 1 isolate of E. coli O157:H7.Houseflies collected from the food court have the potential to transmit antibiotic-resistant E. coli O157:H7 around the IPB campus. |
| 5. | Publisher | Organizing agency, location | Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI |
| 6. | Contributor | Sponsor(s) | Beasiswa Penelitian Tesis Magister BIMA Kemendikbud |
| 7. | Date | (YYYY-MM-DD) | 2024-12-01 |
| 8. | Type | Status & genre | Peer-reviewed Article |
| 8. | Type | Type | |
| 9. | Format | File format | |
| 10. | Identifier | Uniform Resource Identifier | https://journal.ugm.ac.id/jsv/article/view/99843 |
| 10. | Identifier | Digital Object Identifier (DOI) | https://doi.org/10.22146/jsv.99843 |
| 11. | Source | Title; vol., no. (year) | Jurnal Sain Veteriner; Vol 42, No 3 (2024): Desember |
| 12. | Language | English=en | en |
| 13. | Relation | Supp. Files |
Sertifikat Komisi Etik Hewan (682KB) |
| 14. | Coverage | Geo-spatial location, chronological period, research sample (gender, age, etc.) | |
| 15. | Rights | Copyright and permissions |
Copyright (c) 2025 Jurnal Sain Veteriner This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. |