* Corresponding Author
Biomass Production of Root and Shoot of Talinum paniculatum Gaertn. by Liquid and Solid Ms Medium with Plant Growth Hormone IBA
https://doi.org/10.22146/jtbb.13731 Muhammad Hamzah Solim(1*), Y. S. Wulan Manuhara(2)
(1) Department of Biology, Faculty of Science and Technology, Airlangga University
(2) Department of Biology, Faculty of Science and Technology, Airlangga University
(*) Corresponding Author
Abstract
Talinum paniculatum Gaertn. is one of traditional medicinal plant in Indonesia which has benefits such as for vitality and maintain blood circulation. The aim of this research is to obtain biomass production of root and shoot of T. paniculatum Gaertn. by liquid and solid MS medium with IBA. This research conducted to provide biomass as raw material for secondary metabolites test. Stems as explant were induced with four treatments (liquid MS, solid MS, liquid MS + 2 ppm IBA and solid MS + 2 ppm IBA) with five repetitions. Observation did for 28 days. The parameters are the percentage of explants which formed the root and shoot, morphology, fresh and dry biomass. Result shows that percentage of root and shoot have 100% in liquid and solid MS + 2 ppm IBA. Fresh and dry biomass of root and shoot in solid MS + 2 ppm IBA higher than the others. This research found callus in liquid and solid MS + 2 ppm IBA. Morphology of root in liquid MS has thin and friable, but thick in solid MS. Shoot in solid and liquid MS has thin, short and sturdy.
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Adelberg, J., Delgado, M.P. and Tomkins, J.P. 2010. Spent Medium Analysis for Liquid Culture Micropropagation of Hemerocallis on Musrashige and Skoog Medium. In Vitro Cellular and Developmental Biology Plant. 46: 95-107.
Adelberg, J., Fári, M.G. 2010. Applied physiology and practical bioreactors for plant propagation. Propag. Ornam. Plants 10 (4): 205–219.
Baque, M.A., Shiragi, M.H., Moh, S.H., Lee, E.J. and Paek, K.Y. 2014. Production of biomass and bioactive compounds by adventitious root suspension cultures of Morinda citrifolia (L.) in a liquid-phase airlift ballon-type bioreactor. In Vitro Cell. Dev. Biol. Plant 49: 737-749.
Cui, H.Y., Murthy, H.N., Moh, S.H., Cui, Y.Y., Lee, E.J. and Paek, K.Y., 2014, Production of biomass and bioactive compounds in protocorm cultures of Dendrobium candidum Wall ex Lindl. Using ballon type bubble bioreactors, Ind. Crops and Products 53: 28-33.
Davies PJ. The plant hormones: their nature, occurrence and functions. In: Davies PJ, editor. Plant Hormones: Physiology, Biochemistry and Molecular Biology. Dordrecht, The Netherlands: Kluwer Academic Publishers; 1995. pp. 1–12.
Debergh, P.C. (1983). Effects of agar brand and concentration on the tissue culture medium. Physiologiae Plant, 59, 270-276.
Gorret, N., Rosli, S.K.B., Oppenheim, S.F., Wilis, L.B., Lessard, P.A., Rha, C.K. and Sinskey, A.J. 2004. Bioreactor culture of oil palm (Elaeis guineensis) and effects of nitrogen source, inoculums size and conditioned medium on biomass production. J. Biotechnol. 108: 253-263.
Hartmann, H.T., Kester, D.E, Davies, F.T. Jr, Geneve, R.L. Plant Propagation: Principles and Practices. Ed 6. Upper Saddle River, NJ: Prentice Hall; 1997. The biology of propagation by cuttings; pp. 276–328.
Hobbie, L.J. 1998. Auxin: molecular genetic approaches in Arabidopsis. Plant Physiol Biochem. 36: p.91–102. Kuria, P., Demo, P., Nyende, A. and Kahangi, E. 2008, Cassava starch as an alternative cheap gelling agent for the in vitro micro-propagation of potato (Solanum tuberosum L.). African Journal of Biotechnology, 7(3): 301-307.
Ludwig-Muller J. (2000). Indole-3-butyric acid in plant growth and development. J. Plant Growth Regul. 32: 219–230.
Lulu, T., Park, S.Y., Ibrahim, R. and Paek, K.Y., 2015. Production of biomass and bioactive compounds from adventitious roots by optimization of culturing conditions of Eurycoma longifolia in ballon-type bubble bioreactor sistem. Journal of Bioscience and Bioengineering. 119 (6): 712-717.
Mariateresa, C., Maria, C.S.C. and Giuseppe, C. 2014. Influence of ozone treatments on in vitro propagation of Aloe barbadensis in continuous immersion bioreactor. Industrial Crops and Products 55: 194-201.
Manuhara, Y.S.W., Kristanti, A.N. and Utami, E.S.W. 2015, Optimization of Culture Conditions of Talinum paniculatum Gaertn. Adventitious Roots in Balloon Type Bubble Bioreactor Using Aeration Rate and Initial Inoculum Density, Asian Journal of Biological Sciences 8 (2): 83-92.
Mohamed, M.A.H., Alsadon, A.A., Al Mohaidib, M.S. (2009). Corn and potato starch as an agar alternative for Solanum tuberosum micropropagation. African Journal of Biotechnology, 8(19)
Nordstrom A-C., Jacobs, F.A., Eliasson, L. (1991). Effect of exogenous indole-3-acetic acid and indole-3-butyric acid on internal levels of the respective auxins and their conjugation with aspartic acid during adventitious root formation in pea cuttings. Plant Physiol 96: 856–861.
Poupart, J. and Waddell C.S., (2000). The rib1 mutant is resistant to indole-3-butyric acid, an endogenous auxin in Arabidopsis. Plant Physiol 124: 1739–1751.
Prakash, S. (1993). Production of ginger and turmeric through tissue culture methods and investigations into making tissue culture propagation less expensive. Ph.D Thesis. Bangalore University, Bangalore, India. Prakash, S., Hoque, M.I. and Brinks, T. (2000). Culture media and containers. Biotechnology and Eco-development Research Foundation, Bangalore, India. pp. 29-30.
Preece, J.E. 2011, Micropropagation in stationary liquid media. Propagation of Ornamental Plants, 10(4), 183-187.
Rashotte, A.M., Poupart, J., Waddell, C.S. and Muday, G.K. 2003, Transport of the Two Natural Auxins, Indole-3 Butyric Acid and Indole-3-Acetic Acid, in Arabidopsis, Plant Physiology. Vol. 133: p. 761-772.
Sandal, I., Bhattacharya, A., Ahuja, P.S. 2001, An efficient liquid culture system for tea shoots proliferation. Plant Cell Tissue. Organ. Cult. 65: 75-80.
Shaik, S., Dewir, Y.H., Singh, N., Nicholas, A., 2010. Micropropagation and bioreactorstudies of the medicinally important plant Lessertia (Sutherlandia) frutescens L.S. Afr. J. Bot. 76: 180–186.
Thanh, N.T., H.N. Murthy, K. Yu, C.S. Jeong, E. Hahn and K.Y. Paek, 2006. Effect of oxygen supply on cell growth and saponin production in bioreactor cultures of Panax ginseng. J. Plant Physiol., 163: 1337-1341.
Yan, H., Lian, C., Li, Y., 2010. Improved growth and quality of Siraitia grosvenoriiplantlets using a temporary immersion system. Plant Cell Tissue Org. Cult. 103,131–135.
Yang, T. and Davies, P. (1999). Promotion of stem elongation by indole-3-butyric acid in intact plants of Pisum sativum L. J Plant Growth Regul 27: 157–160.
Zolman, B.K., Yoder, A. and Bartel, B. (2000). Genetic analysis of indole-3-butyric acid responses in Arabidopsis thaliana reveals four mutant classes. Genetics 156: 1323–1337.
DOI: https://doi.org/10.22146/jtbb.13731
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