The Effect Of Cadmium on Sperm Quality and Fertilization Of Cyprinus carpio L.

Alfiah Hayati(1*), Khusnita Giarti(2), Yuli Winarsih(3), Muhammad Hilman Fuadil Amin(4)

(1) Departement of Biology, Universitas Airlangga, Surabaya
(2) Departement of Biology, Universitas Airlangga, Surabaya
(3) Departement of Biology, Universitas Airlangga, Surabaya
(4) Departement of Biology, Universitas Airlangga, Surabaya
(*) Corresponding Author


The objective of the study was to determine the effect of cadmium on sperm quality and fertilization of C. carpio L. Sperm and eggs were collected by abdomen striping from the mature testis and ovary of C. carpio L. This study used one control and four treatment groups of variation on the cadmium concentration (0, 50, 100, 150, and 200 ppm) with eight replications. Sperm motility (mass motility, mass motility duration, and individual motility duration) and viability were measured after three to four seconds of incubation in the water. The percentage of fertility success was calculated by observing embryo development after the eggs were mixed with sperm and incubated in the water for 72 hours. The success of the fertilization process was indicated by a color change of the egg that darkens after successful fertilization, and white-milk if failed. The data were analyzed using analysis of variance (α = 0.05). The results of this study indicate that exposure of 50 ppm cadmium and control group shown success in term of sperm quality (motility and viability) and fertilization, but at 100 ppm or more decreased the sperm quality and fertilization rate. It can be concluded that cadmium exposure decreases sperm quality and fertility at 100 ppm or higher concentrations.


Cadmium; sperm quality; fertilization; Cyprinus carpio L.

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Abascal F.J., Cosson J., Fauvel C. 2007. Characterization of sperm motility in sea bass: the effect of heavy metals and physicochemical variables on sperm motility. J. Fish. Biol. 70: 509-522.

Agarwal, A., G. Virk, C. Ong, and SS Plessis, 2014, Effect of Oxidative Stress on Male Reproduction, World J Mens Health, 32(1): 1–17.

Cabrita, E., Martínez-Páramo, 324 S., Gavaia, P. J., Riesco, M.F., Valcarce, D.G., Sarasquete, C., Herráez, M. P., Robles, V., 2014. Factors enhancing fish sperm quality and emerging tools for sperm analysis. Aquaculture 432, 389–401.

Connel dan Miller, 1995, Kimia dan Etoksikologi Pencemaran, diterjemahkan oleh Koestoer, S., hal. 419, Indonesia University Press, Jakarta.

Cosson, J., 2004. The ionic and osmotic factors are controlling motility of fish spermatozoa. 331 Aquacult Int. 12, 69–85.

Coward K., Bromage N.R., Hibitt O., Parrington J. 2002. Gamete physiology, fertilization and egg activation in teleost fish. Rev. Fish Biol. Fish. 12: 33-58.

Dandan, Ma. et al. 2013. Oxidative damages and ultrastructural changes in the sperm of fresh water crab Sinopotamon henanense exposed to cadmium. Vol 98. Hal 244–249.

Darmono. 2001. Lingkungan hidup dan pencemaran hubungannya dengan toksikologi senyawa logam. UI Press: Jakarta.

Dietrich, G.J., Szpyrka, A., Wojtczak, M., Dobosz, S., Goryczko, K., Z˙ akowski, Ł., Ciereszko, A., 2010. Effects of UV irradiation and hydrogen peroxide on DNA fragmentation, motility and fertilizing ability of rainbow trout (Oncorhynchus mykiss) spermatozoa. Theriogenology 64, 1809–1822.

Dzyuba, V., Cosson, J., 2014. Motility of fish spermatozoa: from external signalling to flagella response. Reprod. Biol. 14, 165-175.

Fraser, L., Strzeżek, J.,Kordan,W. 2011. Effect of freezing on sperm nuclear DNA. Reprod. Domest. Anim. 46,14–16.

Ginzburg, A. S. 1972. Fertilization in fishes and problem of polyspermy. T. A. Detlaf (ed). Winer Bidery Ltd. Jerusalem.

Herdish, M. R. Toelihere, I. Supriyatna, B. Puwantara, dan T. T. S. Adikara. 2005. Optimalisasi Waktu Ekuilibrasi dan Metode Pencairan Kembali pada Proses Pembekuan Semen Domba Garut (Ovis aries). Journal Animal Production Vol 7. Hal. 81-88.

Hermawaty, D. 2008. Prosedur Pemijahan/Budidaya Ikan Black Ghost (Apteronotus albifrons). Program Studi Biologi Sekolah Ilmu dan Teknologi Hayati. Institut Teknologi Bandung.

Jezierska B, Witeska, M. 2001. Metal Toxicity to Fish. University of Podlasie Publisher, Siedlce, p 318

Lahnsteiner, F., Berger, B., Weismann, T., 1999. Sperm metabolism of the teleost fishes Oncorhynchus mykiss and Chalcalburnus chalcoides and its relation to motility and viability. J. Exp. Zool. 284, 454–465.

Lahnsteiner, F., Mansour, N., Berger, B., 2004. The effect of inorganic and organic pollutants on sperm motility of some freshwater teleosts. J. Fish Biol. Vol 65, 1283–1297.

Li, P., 395 Li, Z. H., Dzyuba, B., Hulak, M., Rodina, M., Linhart, O., 2010. Evaluating the impacts of osmotic and oxidative stress on common carp (Cyprinus carpio, L.) sperm caused by cryopreservation techniques. Biol. Reprod. Vol 83, 852–858.

Mason, CF. 1981. Biology of Freshwater Pollution. Longman lnc, New York.

Mishra, S., Srivastava, S., Tripathi, R.D., Govindarajan, R., Kuriakose, S.V., Prasad, M. N.V., 2006. Phytochelatin synthesis and response of antioxidants during cadmium stress in Bacopa monnieri L. Plant Physiol. Biochem. 44,25–37.

Monsefi M., Alaee S., Moradshahi A., Rohani L. 2009. Cadmium-induced male infertility in male mice. Environ. Toxicol. 25: 94-102.

Morisawa, M., Morisawa, S., 1990. Aquisition and initiation of sperm motility, in: Gagnon, 409 C. (Eds.), Controls of sperm motility: biological and clinical aspects. Boca Raton, FL: CRC 410 Press, pp. 137–152.

Morita T., Nakada K., Sato A., Yoshida K. et al. 2003. Mitochondria – Related Male Infertility. PNAS.

Nichi, M., Bols, P.E. J., Zuge, R.M., Barnabe, V.H., Goovaerts, I.G.F., Barnabe, R.C., Cortada, C.N.M., 2006. Seasonal variation in semen quality in Bos indicus and Bos taurus bulls raised under tropical conditions. Theriogenology. Vol 66, 822-828.

Palar H. 2002. Pencemaran dan Toksikologi Logam Berat. Jakarta : Rineka Cipta

Patric, L. 2006. Lead toxicity, a review of the literature. Part I. Exposure, evaluation and treatment. Altern. Med. Rev.11,2–22.

Reddy RS, Jinna RR, Uzodinma JE, Desaiah D. 1988. In vitro effect of mercury and cadmium on brain Ca2?-ATPase of the catfish Ictalurus punctatus. Bull Environ Contam Toxicol 41:324–328.

Rizal, M., dan Herdis. 2005. Daya Hidup Spermatozoa Epididimis Domba Garut yang Dikriopreservasi Menggunakan Modifikasi Pengencer Tris. Jurnal Hayati ISSN 0854-8587. Hal. 62-66.

Setyono, B. 2009. Pembenihan Ikan. 17 Juni 2017. 1 hal.

Siu, E.R, Mruk, D.D., Porto, C.S., Cheng, C.Y. 2009. Cadmium-induced testicular injury. Toxicol. Appl. Pharmacol. 238,240–249.

Tandjung, HSD. 1992. Pengaruh kadmium klorida kadar rendah terhadap Spermatogenesis lkan. Biologi. I:159-167.

Valko, M., Rhodes, C.J., Moncol, J., Izakovic, M., Mazur, M. 2006. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem. Biol. Inter - act. 160,1–40.

Warnecke, D., 2005. Motility, ATP content, and membrane integrity of fresh and cryopreserved spermatozoa of carp (Cyprinus carpio L.) and sterlet (Acipenser ruthenus L.) as endpoints for the evaluation of aquatic environmental chemicals. PhD thesis. Freie Universitat, Berlin.

Woynarovich E dan L Horvath. 1980. The Artificial Propagation of Warmwater Fishes Manual Extention. FAO Fisheries Technical Paper No. 201. : 183 pp. Roma.


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