Kandungan Fenolik, Flavonoid dan Aktivitas Antioksidan Ekstrak Daun Paku Laut (Acrostichum aureum L.) Fertil dan Steril di Kawasan Mangrove Kulon Progo, Yogyakarta


Naovi Nur Fadia Hanin, Rarastoeti Pratiwi,

(1) Universitas Gadjah Mada
(2) Faculty of Biology, Universitas Gadjah Mada


Sea Fern (Acrostichum aureum L.), which typically grows in mangrove ecosystem, has two distinguished leaves which are fertile leaves (rich in spores) and sterile leaves (no spores). Those leaves are known rich in phenolic compounds. Flavonoids, moreover, which belong to class of phenolic compound, have antioxidant activity. This study aims were to analyze the phenolic content, flavonoid content, and antioxidant activity of fertile leaves, sterile leaves, and spores of sea fern which grow in mangrove ecosystem that is located in Kulon Progo, Yogyakarta. Leaf and spore samples were extracted using ethanol solvent. Then, total content of phenol and flavonoid were measured by spectrophotometry, while the antioxidant activity was measured by DPPH method. The results showed that the phenolic content of fertilized leaves and sterile leaves did not show a significant difference. In other hand, the spores sample showed highest both of flavonoids content (2.32 mg/mL) and the antioxidant activity with the IC50 value was 23.25 ppm. Thus, the ethanolic extracts of spores of sea fern sterile leaves showed the highest flavonoid content and could be categorized as a very strong antioxidant.


antioxidant; flavonoid; phenolic compounds; spores; Acrostichum aureum L.

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Anwar, K., Fadlillaturrahmah, Sari, D.P., 2017, Analisis Kandungan Flavonoid Ekstrak Etanol Daun Binjai (Mangifera caesia Jack.) dan Pengaruhnya terhadap Kadar Glukosa Darah Tikus yang Diinduksi Fruktosa-Lemak Tinggi, Jurnal Ilmiah Ibnu Sina 2(1), 20-30.

Anwar, K., Rahmanto, B., Triyasmono, L., Rizki, M. I., Halwany, W & Lestari, F., 2017, The Influence of Leaf Age on Total Phenolic, Flavonoids, and Free Radical Scavenging Capacity of Aquilaria beccariana, Research Journal of Pharmaceutical, Biological and Chemical Sciences, 8(1S), 129-133.

Aziz, T., Febrizky, S., Mario, A.D., 2014, Pengaruh Jenis Pelarut Terhadap Persen YIELD ALKALOID dari Daun Salam India (Murayya koenigii), Teknik Kimia 20(2), 1-6.

Biswal, U.C., Biswal, B., & Raval M.K., 2003, Chloroplast Biogenesis: From proplastid to gerontplast, The Netherlands, Kluwer Academic Pubisher, Dordrecht.

Chaovanalikit A., Wrolstad, R.E., 2004, Total anthocyanins and total phenolics of fresh and processed cherries and their antioxidant properties, Food Chemistry and Toxicology, 69 (2004), 67–72.

Dia, S.P.S., Nurjanah & Jacoeb, A.M., 2016, Komposisi Kimia dan Aktivitas Antioksidan Akar, Kulit Batang dan Daun Lindur, JPHPI 2015 18(2), 209.

Firdiyani, F., Agustini, Tri W., & Ma’ruf, W.F., 2015, Ekstraksi Senyawa Bioaktif sebagai Antioksidan Aami Spirulina platensis Segar dengan Pelarut yang Berbeda. JPHPI 18(1).

Forest Practice Branch Ministry of Forest,  2012,  Operational Summary for Vegetation Complex: Fern complex. ISBN 0-7726-4718-6. Province of British Columbia.

Garg, N., Abdel-Aziz, S.M., & Aeron, A., 2016, Microbes in Food and Health, Springer, Switzerland 42-45.

Hossain, H., jahan, I.A., Nimmi, I., Hossain, Md.A., & Kawsar, Md.H., 2011. Anti-Inflammatory Activity of The Ethanolic Extract of Acrostichum aureum (Linn.) root, Bangladesh Pharmaeutical Journal 14(2), 107-109

Harborne, J.B., Williams C.A., (2000), Advance in Flavonoid research since 1992, Phytocemistry 55: 481-504.

Ibrahim, M.H., Jaafar, H.Z., 2012, Primary, Secondary Metabolites, H202, Malondialdehyde and Photosynthetic Response of Orthosiphon stimaneus Benth. To Different Irradiance Levels. Molecules 17, 1159-1176

Indrawati, Ni Luh., Razimin., 2013, Bawang Dayak : Si Umbi Ajaib Penakluk Aneka Penyakit. PT AgroMedia Pustaka. Jakarta.

Khan, S.A., Hossain, A., Panthi, S & Asadujjaman, H.A., 2013, Assesment of antioxidant and Analgesic activity of Acrostichum aureum Linn. (Family Pteridaceae).  Pharmacology Online 1, 166-17.

Lai, Y.H., Lim Y.Y., 2011, Evaluation of Antioxcidant Activities of the Methanolic Extract of Selected Ferns in Malaysia. IPCBEE 20.

Li, Q., Kubota, C., 2009, Effect of Suplemental Light Quality on Growth and Phytochemicals of Baby Leaf Lettuce, Exp. Exp. Bot 67,59-64.

Lobo, S.M., G, Khrisnakumar., 2014, Studies on Ecological Anatomy of The Mangrove Fern Acrostichum Aureum L., International Journal of Plant, Animal and Environtment Sciences 4(1), 195-200.

Molyneux, P., 2003, The Use of The Stable Free Radical Diphenylpicrilhydrazyl (DPPH) for Estimating Antioxidant Activity. J.Sci. Technol 26(2), 211-219.

Neldawati, Ratnawulan, & Gusnedi, 2013, Analisis Nilai Absorbansi dalam Penentuan Kadar Flavonoid untuk Berbagai Jenis Daun Tanaman Obat, Pillar of Physics 2, 76-83.

Prakash, A., 2001, Antioxidant Activity, Medallion Laboratories analytical progress 19 (2), 1-6.

Pekal, A., Pyrzynska, K., 2014, Evaluation of Aluminium Complexation Reaction for Flavonoid Content Assay, Food Anal. Methods (2014) 7, 1776-1728.

Putri, A.S.P., Hidajati, N., 2015, Uji aktivitas antioksidan senyawa fenolik ekstrak methanol kulit batang tumbuhan Nyiri batu (Xylocarpus moluccencis), Unesa Journal of chemistry 4(1), 37-42.

Ragavan, P., Saxena, A., Mohan, P.M., & Ravinchandran, K., 2014, A Hybrid of Acrostichum from Andaman and Nicobar Island India, ISME/GLOMIS Electronic Journal 12(3), 9-13.

Raja, S., Ravindranadh, K., 2014, A Complete Profile on Acrostichum Aureum : Traditional Uses, Pharmacological activities and Phytoconstituens, World Journal of Pharmaceutical Research 3(10), 624-630

Raja, S. Ravindranadh, K., 2014, Preliminary phytochemical screening of different solvent extracts of whole plants of Acrostichum aureum, World Journal of Pharmaceutical Sciences, 2(12), 1753-1759.

Rhodes, M.J.C., Yemm, E.W., 1965, The metabolism of Nucleic Acids and Proteins in young leaves of Barley. Biochim, biophys.Acta. (In Press).

Sarastani, D., Soekarto S.T., Muhchtadi T.R., Fardiaz, D & Apriyantono, A., 2002, Aktivitas antioksidan ekstrak dan fraksi ekstrak Biji Atung (Parinarium glaberrimum Hassk.). Jurnal Teknologi dan Industri Pangan 13(2), 149-156.

Shebis, Y., ILuz, D., Tahan, Y.K., Dubinsky, Z & Yehoshua, Y., 2013. Natural Antioxidant : Function and Sources. Food and Nutrition Sciences 4, 634-649

Ukoha, P.O., Cemaluk, E.A.C., Nnamdi, O.L & Madus, E.P., 2011, Tannins and other phytochemical of the Samanaea saman pods and their antimicrobial activities. African Journal of Pure and Applied Chemistry 5(8), 237-244.

Yuhernita., Juniarti., 2011, Analisis senyawa Metabolit Sekunder dari Ekstrak Metanol Daun Surian yang Berpotensi sebagai Antioksidan, Makara Sains, 15(1), 48-52.

DOI: https://doi.org/10.22146/jtbb.29819

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