The Effect of Ethanolic Extract of Cashew Fruit Peel on The Liver Histological Structure in Rat (Rattus norvegicus Berkenhout, 1769)

https://doi.org/10.22146/jtbb.39275

Laili Mufli Zusrina(1), Bambang Retnoaji(2*), Nastiti Wijayanti(3)

(1) Faculty of Biology, Universitas Gadjah Mada
(2) Faculty of Biology, Universitas Gadjah Mada
(3) Faculty of Biology, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Cashew fruit peel is a waste produced from the cashew nut industry, and it has not been utilized optimally yet. Cashew peel extract has the potential to be used as a contraceptive agent, which capable of reducing reproductive capacity. However, its side effects on other tissue and organ such as liver not clearly studied yet. This study aims to determine the effect of ethanolic extracts of cashew peel on the histological structure of the white rat liver. In this study, 21 female white rats were used and be grouped for control (6 mice) which were treated with CMCMa 0.5% and 15 mice were treated with peel extract of 500 mg/kg body every day for one month. Liver for examination was collected sequentially at 3rd, 5th, 8th, 11th, and 14th of the estrous cycle. The liver was processed for histological observation and stained with Hematoxylin Eosin and Mallory Acid Fuchsin staining solution. The liver hepatocyte was observed for it abnormality and be scored to calculate the number of cell damage or abnormality. The result showed that peel extract-treated mouse liver was similar to control ones; we did not witness any evidence of fibrosis, pyknosis and cellular necrosis on either control or treated mouse. Statistical analysis by SPSS showed that the p-value between the control and treatment groups was 0.078 (> 0.05) so there was no significant difference between control and treatment. It could be concluded that ethanolic extracts of cashew nuts peel with a concentration of 500 mg/kg body weight caused no effect on the mouse liver histological structure. application with reduced-dosages of NPK fertilizers were arranged in a random block design with three replicates. The results show that large quantities of silica bodies attached to the surface of EFB fibers and amounting to 0.44% soluble Si. The FFB data indicated that the application of 75% NPK + 500 kg composted EFB + 2 L BioSilAc/ha/year on a five-year-old plant resulted in higher yield than that obtained from 100% standard dosage of NPK. The study also revealed that the application of EFB compost reduced 50% of BioSilAc dosage.


Keywords


cashew fruit; peel; extract; mouse; liver; histology

Full Text:

PDF


References

Carlton, W.W. & McGavin, M.D., 1995, Special Veterinary Pathology, 2nd ed., Mosby, United States of America.

Chiang, J., 2014, ‘Liver Physiology: Metabolism and Detoxification’, in L.M. McManus & R.N.. Mitchell (eds), Pathobiology of Human Disease, pp. 1770-1782,  Elsevier, San Diego.

Bedossa, P., 1994,  Intraobserver & Interobserver Variations in Liver Biopsy Interpretation in Patients with Chronic Hepatitis C, Hepatology 20(1), 15-20.

BPS, 2002, Statistik Perkebunan, Jakarta.

Harlita, 2016, Potensi Ekstrak Kulit Biji Mete (Anacardium occidentale L.) Sebagai Agen Penurun Kapasitas Reproduksi Tikus Putih (Rattus norvegicus Berkenhout 1769) betina [Potential of Cashew Seed Extract (Anacardium occidentale L.) As a Reducing Reproductive Capacity of White Rats (Rattus norvegicus Berkenhout 1769) female], Doctoral thesis, pp. 2-14, Universitas Gadjah Mada, Yogyakarta.

Herlina, 2013, Potensi Biotoksikan Ekstrak Etanolik Kulit Biji Mete (Anacardium occidentale L.) Pada Sistem Reproduksi Jantan Tikus Putih (Rattus norvegicus Berkenhout 1769) Galur Wistar [Biotoxic Potential of Ethanolic Extract of Cashew Seed Skin (Anacardium occidentale L.) in Male Reproduction System of White Rat (Rattus norvegicus Berkenhout 1769) Wistar Strain] Master thesis, pp. 122-132, Universitas Gadjah Mada, Yogyakarta.

Hou, L., Kai, L., Yuhong, L., Shuang, M., Xunming, J. & Lei, L., 2016, Necrotic Pyknosis is a Morphologically and Biochemically Distinct Event from Apoptotic Pyknosis, Journal of Cell Science 129, 3084-3090.

King, N.W. & Joseph, A., 1996, ‘Intracellular and Extracellular Deposition;  Degenerations’, in T.C. Jones, R.D. Hunt & N.W. King (eds.)Veterinary Pathology 6th ed., Blackwell Publishing Professional, USA.

Patel, S., 2016, Emerging Adjuvant Therapy for Cancer; Propolis and Its Constituents, J Diet Suppl. 13:245-268

Sativani I., 2010, Pengaruh Pemberian Deksametason Dosis Bertingkat Per Oral 30 Hari Terhadap Kerusakan Sel Hepar Tikus Wistar [The Effect of Giving Dexamethasone 30-Day Oral Dose to Wistar Rat Cell Damage], Undergraduate thesis, Universitas Diponegoro, Semarang.



DOI: https://doi.org/10.22146/jtbb.39275

Article Metrics

Abstract views : 162 | views : 112

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 Journal of Tropical Biodiversity and Biotechnology

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Editoral address:

Faculty of Biology, UGM

Jl. Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia

ISSN: 2540-9581 (online)