The Antidepressant Effect of Chlorella vulgaris on Female Wistar Rats (Rattus norvegicus Berkenhout, 1769) with Chronic Unpredictable Mild Stress Treatment

https://doi.org/10.22146/jtbb.43967

Gisella Intan Soetantyo(1*), Mulyati Sarto(2)

(1) Faculty of Biology, Universitas Gadjah Mada
(2) Faculty of Biology, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Depression is a disabling mental disorder, predicted to become the world's number 2 disability by 2020 by the World Health Organization (WHO, 2018). Chronic stress is one of the triggers for depression, causing an imbalance in brain chemicals and antioxidants levels. Although antidepressant is a common treatment, discomforting side effects has compromised its efficacy, prompting the search for alternative medicines. Chlorella vulgaris is a microalgae famous for its excellent protein and antioxidant content. In this study, C. vulgaris (360 mg/kg p.o.) potency of antidepressant in chronic unpredictable mild stress (CUMS) model of depression in female rats was evaluated compared to amitriptyline (2,25 mg/kg p.o.) for 14 days. Two types of C. vulgaris namely cultivation sourced and commercially-sold, were used. Sucrose preference test, forced swim test (FST) and open field test (OFT) were used as depression-like behaviour test to validate C. vulgaris effect. Adrenal glands were observed to further understand its effect on the stress organ. The CUMS method produced rats with depressive-like behaviour evidently by reduced body weight, sucrose preference, exploring behaviour in OFT, and increased immobility duration in FST. Furthermore, an increase in adrenal weight, fasciculata zone, and reticularis zone was observed. Both C. vulgaris significantly (p<0,05) reversed depressive-like behaviour in rats subjected to CUMS, but not the size of adrenal glands. This finding indicated both types of C. vulgaris has the potential to be an alternative antidepressant but because of the short duration of treatment, it’s speculated that C. vulgaris may not have exhibited enough difference structurally yet.

 


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References

Avinashe, A.M., 2013, Role of adrenal gland in the gonadosomatic index of male Calotesversicolor (Boulenger), Vidyabharati International Interdisciplinary Research Journal 2(1), 71-75.

Barnett, S.A., 2017, The Rat: A Study in Behavior, Routledge, NY, pp. 124

Belovicova, K., Bogi, E., Csatlosova, K., & Dubovicky, M., 2017, Animal tests for anxiety-like and depression-like behavior in rats, Interdisciplinary Toxicology 10(1), 40-43.

Bewicke, D. & Potter, B.A., 2009, Chlorella: The Emerald Food, Ronin Publishing, California, pp. 1-26.

Che, Y., Zhou, Z., Shu, Y., Zhai, C., Gong, S., Cui, Y. & Wang, J.F., 2015, Chronic unpredictable stress impairs endogenous antioxidant defense in rat brain, Neuroscience Letters 584, 208-13.

Chung, S., Hoon Son, G. & Kim, K., 2011, Circadian rhythm of adrenal glucocorticoid: Its regulation and clinical implications, Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 1812 (5), 581-591.

Cipriani, A., Furukawa, T.A., Salanti, G., Chaimani, A., Atkinson, L.Z., Ogawa, Y., Leucht, S., Ruhe, H.G., Turner, E.H., Higgins, J.P., Egger, M., Takeshima, N., Hayasaka, Y., Imai, H., Shinohara, K., Tajika, A., Ioannidis, J.P.A. & Geddes, J.R., 2018, Comparative efficacy and acceptability of 21 antidepressant drugs for the acute treatment of adults with major depressive disorder: a systematic review and network meta-analysis, The Lancet 17, 32802-7.

Goddard, A.W., Ball, S.G., Martinez, J., Robinson, M.J., Yang, C.R., Russell, J.M. & Shekhar, A., 2009, Current perspectives of the roles of the central norepinephrine system in anxiety and depression, Depression and Anxiety 27(4), 339-350.

Gupta, G., Jia, T.J., Woon, L.Y., Chellappan, D.K., Candasamy, M. & Dua, K., 2015, Pharmacological Evaluation of Antidepressant-Like Effect of Genistein and Its Combination with Amitriptyline: An Acute and Chronic Study, Advances in Pharmacological Sciences 2015, 1-6.

Hammen, C., 2005, Stress and depression, Annual Review of Clinical Psychology 1, 293-319.

Hedaya, R.J., 2011, The Antidepressant Survival Guide: The Clinically Proven Program to Enhance the Benefits and Beat the Side Effects of Your Medication, Three Rivers Press, N.Y.

Hemat, R.A.S., 2004, Principles of Orthomolecularism, Urotext, London, p 37.

Hsu, C., Chao, P., Hu, S. & Yang, C., 2013, The Antioxidant and Free Radical Scavenging Activities of Chlorophylls and Pheophytins, Food and Nutritions Sciences 4, 1-8.

Hu, C., Luo, Y., Wang, H., Kuang, S., Liang, G., Yang, Y., Mai, S. & Yang, J., 2017, Re-evaluation of the interrelationships among the behavioral tests in rats exposed to chronic unpredictable mild stress, Plos One 12(9), e0185129.

Klamczynska, B. & W. D. Mooney. 2017. Sustainable Protein Sources. Academic Press. California. pp 327-33.

Lopez-Munoz, F. & Alamo, C., 2011, Neurobiology of Depression, CRC Press, California, pp. 143-159.

Moret, C. & Briley, M., 2011, The importance of norepinephrine in depression, Neuropsychiatric Disease and Treatment 7(Suppl 1), 9-13.

Natarajan, R., Northrop, N. A. & Yamamoto, B. K., 2015, Protracted effects of chronic stress on serotonin-dependent thermoregulation, Stress 18(6), 668-676.

Perez-Escudero, A., Vicente-Page, J., Hinz, R.C., Arganda, S. & de Polavieja, G.G., 2014, idTracker: tracking individuals in a group by automatic identification of unmarked animals, Nature Methods 11(7), 743-8.

Preiser, J, 2016, The Stress Response of Critical Illness: Metabolic and Hormonal Aspects, Springer, California.

Rabasa, C. & Dickson, S.L., 2016, Impact of stress on metabolism and energy balance, Behavioral Sciences 9, 71-77.

Safi, C., Zebib, B., Merah, O., Pontalier, P. & Vaca-Garcia, C., 2014, Morphology, composition, production, processing and applications of Chlorella vulgaris: A review, Renewable and Sustainable Energy Reviews 35, 265-78.

Santhanam, R., 2015, Nutritional Freshwater Life, CRC Press, California, pp.9.

Saranya, C., Hemalatha, A., Parthiban, C., Anantharaman, P., 2014, Evaluation of Antioxidant Properties, Total Phenolic and Carotenoid Content of Chaetoceros calcitrans, Chlorella salina and Isochrysis galbana, International Journal of Current Microbiology and Applied Sciences 3(8), 365-377.

Scapagnini, G., Davinelli, S., Drago, F., De Lorenzo, A. & Oriani, G., 2012, Antioxidants as Antidepressants: Fact or Fiction?, CNS Drugs 26(6), 477-90.

Serchov, T., van Calker, D., & Biber, K., 2016, Sucrose Preference Test to Measure Anhedonic Behaviour in Mice, Bio-protocol 6(19), e1958. DOI: 10.21769/BioProtoc.1958.

Shukkoor, M.S.A., Bin Baharuldin, M.T.H., Mat Jais, A.M., Moklas M.A.M. & Fakurazi S., 2016, Antidepressant-Like Effect of Lipid Extract of Channa striatus in Chronic Unpredictable Mild Stress Model of Depression in Rats, Evidence-Based Complementary and Alternative Medicine 2016, 1-17

Slattery, D.A. & Cryan, J.F., 2012, Using the rat forced swim test to assess antidepressant-like activity in rodents, Nature Protocols 7(6),1009-1014.

Srivastava, K.K. & Kumar, R., 2015, Stress, oxidative injury and disease, Indian Journal of Clinical Biochemistry 30(1), 3-10.

Tiller, J.W.G., 2013, Depression and anxiety, Medical Journal of Australia 199 (6 Suppl), S28-S31.

Ulrich-Lai, Y.M., Figueiredo, H.F., Ostrander, M.M., Choi, D.C., Engerland, W.C. & Herman, J.P., 2006, Chronic stress induces adrenal hyperplasia and hypertrophy in a subregion-specific manner, American Journal of Physiology-Endocrinology and Metabolism 291(5), 965-73.

Wang, S., Hu, S., Zhang, C., Qiu, J. & Li, Y., 2014, Antidepressant-like activity of Chaihu-Shugan-San aqueous extract in rats and its possible mechanism, Pharmacognosy Magazine 10(Suppl 1), 50-56.

WHO, 2018, ‘Depression’, viewed 23 March 2019, from https://www.who.int/news-room/fact-sheets/detail/depression.

Yau, Y.H.C & Potenza, M.N, 2014, Stress and Eating Behaviors, Minerva Endocrinology 38(3), 255-267.

Zhang, L., Luo, J., Zhang, M., Yao, W., Ma, X. & Yu, S.Y., 2014, Effects of curcumin on chronic, unpredictable, mild, stress-induced depressive-like behavior and structural plasticity in the lateral amygdala rats, International Journal of Neuropsychopharmacology 17(5), 793-806.

Zheng H., Yin, J., Gao, Z., Huang, H., Ji, X. & Dou, C., 2011, Disruption of Chlorella vulgaris cells for the release of biodiesel-producing lipids: a comparison of grinding, ultrasonication, bead milling, enzymatic lysis, and microwaves, Applied Biochemistry and Biotechnology 164, 1215–24.

Zoladz, P.R., Fleshner, M. & Diamond, D.M., 2013, Differential effectiveness of tianeptine, clonidine and amitriptyline in blocking traumatic memory expression, anxiety and hypertension in an animal model of PTSD, Progress in Neuro-Psychopharmacology & Biological Psychiatry 44, 1-16.



DOI: https://doi.org/10.22146/jtbb.43967

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