Intergeneric Hybridization between Phalaenopsis 2166 and Vanda ‘saint valentine’: Characterization of Parents Using ndhE cpDNA Partial Sequence

Murni Dwiati(1*), Agus Hery Susanto(2)

(1) Faculty of Biology Jenderal Soedirman University
(2) Faculty of Biology Jenderal Soedirman University
(*) Corresponding Author


An intergeneric cross between Phalaenopsis 2166 and Vanda ‘saint valentine’ has successfully produced protocorms that will be grown further to form seedlings. The present study aims to genetically characterize both parents by using ndhE partial gene as its sequence is shown polymorphic among five orchid genera of the subtribe Oncidiinae. The results reveal that the ndhE partial sequences of Phalaenopsis 2166 and Vanda ‘saint valentine’ are considerably homologous with those of Oncidium. However, alignment of ndhE partial sequences between both parents shows only 58% similarity, leading to the conclusion that a relatively high genetic difference between them may occur.



intergeneric hybridization, Phalaenopsis 2166, Vanda ‘saint valentine’, ndhE partial gene

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Akita, S. et al., 2021. Molecular evidence for naturally occurring intra- and inter-generic hybridization in the genus Ecklonia (Laminariales, Phaeophyceae). Phycologia, 60(2), pp.170–179.

Dong, W.L. et al., 2018. Molecular evolution of chloroplast genomes of orchid species: Insights into phylogenetic relationship and adaptive evolution. International Journal of Molecular Sciences, 19(3).

Doyle, J.J. & Doyle, J.L., 1990. Isolation of plant DNA from fresh tissue. Focus, 12(1), pp.13–15.

Filiz, E. & Koc, I., 2014. Assessment of chloroplast microsatellite from pine family (Pinaceae) by using bioinformatics tools. Indian Journal of Biotechnology, 13, pp.34–40.

Hall, T.A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Series, 41, pp.95 – 98.

Hartati, S., 2010. The intergeneric crossing of Phalaenopsis sp . and Vanda tricolor. Journal of Biotechnology and Biodiversity, 1(1), pp.32–36.

Jheng, C. et al., 2012. Plant Science The comparative chloroplast genomic analysis of photosynthetic orchids and developing DNA markers to distinguish Phalaenopsis orchids. Plant Science, 190, pp.62–73.

Johnson, T.R. & Kane, M.E., 2007. Asymbiotic germination of ornamental Vanda : in vitro germination and development of three hybrids. Plant Cell, Tissue and Organ Culture, 91, pp.251–261.

Kim, D.G., Kim, K.K. & Been, C.G., 2015. Development of intergeneric hybrids between wind orchids (Sedirea japonica and Neofinetia falcata) and moth orchids (Phalaenopsis alliances). Hoticultural and Environmental Biotechnology, 56(1), pp.67–78.

Kim, H.T. & Chase, M.W., 2017. Independent degradation in genes of the plastid ndh gene family in species of the orchid genus Cymbidium (Orchidaceae; Epidendroideae). PLoS ONE, 12(11), e0187318.

Kim, Y.K. et al., 2020. Plastome evolution and phylogeny of Orchidaceae, with 24 new sequences. Frontiers in Plant Science, 11(22).

Kishor, R. & Sharma, G.J., 2008. Synthesis of ornamental hybrid orchids using rare and endangered species. Acta Horticulturae, 788, pp.77–85.

Kishor, R. & Sharma, G.J., 2009. Intergeneric hybrid of two rare and endangered orchids, Renanthera imschootiana Rolfe and Vanda coerulea Griff. Euphytica, 165(2), pp.247–256.

Luo, J. et al., 2014. Comparative chloroplast genomes of photosynthetic orchids: Insights into evolution of the Orchidaceae and development of molecular markers for phylogenetic applications. PLoS ONE, 9(6), e99016.

Niu, Z. et al., 2017. The complete plastome sequences of four orchid species: Insights into the evolution of the Orchidaceae and the utility of plastomic mutational hotspots. Frontiers in Plant Science, 8(715).

Pan, I. et al., 2012. Complete chloroplast genome sequence of an orchid model plant candidate: Erycina pusilla apply in tropical Oncidium breeding. PLoS ONE, 7(4), e34738.

Peredo, E.L., King, U.M. & Les, D.H., 2013. The plastid genome of Najas flexilis: Adaptation to submersed environments is accompanied by the complete loss of the NDH complex in an aquatic Angiosperm. PLoS ONE, 8(7), e68591.

Sanger, F. & Nicklen, S., 1977. DNA sequencing with chain-terminating. PNAS, 74(12), pp.5463–5467.

Sharma, S. et al., 2013. A compendium of monopodial orchids of Meghalaya. Keanean Journal of Science, 2, pp.93-106.

Smidt, E.D.C. et al., 2020. Characterization of sequence variability hotspots in Cranichideae plastomes (Orchidaceae, Orchidoideae). PLoS ONE, 15(1), e0227991.

Sui, C. et al., 2018. The complete chloroplast genome of Paphiopedilum tranlimianum (Orchidaceae). Mitochondrial DNA Part B: Resources, 3(2), pp.820–822.

Thompson, J.D., Higgins, D.G. & Gibson, T.J., 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acid Research, 22, pp.4673–4680.

Wang, S., Shi, C. & Gao, L., 2013. Plastid genome sequence of a wild woody oil species, Prinsepia utilis, provides insights into evolutionary and mutational patterns of Rosaceae chloroplast genomes. PLoS ONE, 8(9), e73946.

Widyastoety, D., Solvia, N. & Soedarjo, M., 2010. Potensi anggrek Dendrobium dalam meningkatkan variasi dan kualitas anggrek bunga potong. Jurnal Litbang Pertanian, 29(3), pp.101–106.

Wu, F. et al., 2010. Complete chloroplast genome of Oncidium Gower Ramsey and evaluation of molecular markers for identification and breeding in Oncidiinae. BMC Plant Biology, 10(68), pp.1–12.

Zhu, Z.X. et al., 2019. Complete plastome sequences of two Neottia species and comparative analysis with other Neottieae species (Orchidaceae). Folia Geobotanica, 54(3–4), pp.257–266.


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