Essential Oils Composition of Kaffir Lime (Citrus hystrix DC.) Collection of Bogor Botanic Gardens from Central Java and East Sumba

Inggit Puji Astuti(1*), Kartika Dyah Palupi(2), Frisca Damayanti(3)

(1) Research Center for Plant Conservation and Botanic Gardens, National Research and Innovation Agency, Jl. Ir. H. Djuanda No.13 Bogor 16122, West Java, Indonesia
(2) Research Center for Chemistry, National Research and Innovation Agency, Gd. 452 PUSPIPTEK, Tangerang Selatan 15314, Banten, Indonesia
(3) Research Center of Plant Conservation and Botanic Gardens, Indonesian Institute of Science
(*) Corresponding Author


The Bogor Botanical Gardens' Citrus hystrix collections from East Sumba and Central Java differ in morphology and fruit aroma compared to the common C. hystrix. Hence, this study aimed to determine the essential oils' compositions of C. hystrix originated from Central Java and East Sumba to further clarify these differences. Extraction of essential oils were done using hydro-distillation, and the chemical compositions were investigated using GC-MS. The main compound of the leaf oil from East Sumba and Central Java was Linalool and Citronellal, respectively. Meanwhile, the main constituents were almost identical for the fruit oils, namely L-β-Pinene, D-Limonene, and L-α-Terpineol.



aromatic constituents; fruit oil; hydro-distillation method; leaf oil; kaffir lime

Full Text:



Amaral, R.R. et al., 2013. Essential oils from fruit with different colors and leaves of Neomitranthes obscura (DC.) N. Silveira: An endemic species from Brazilian Atlantic Forest. BioMed Research International, Article ID 723181, 7 pages. doi: 10.1155/2013/723181

Ampasavate, C., Okonogi, S. & Anuchapreeda, S., 2010. Cytotoxicity of extracts from fruit plants against leukemic cell lines. African Journal of Pharmacy and Pharmacology, 4(1), pp.013-021. doi: 10.5897/AJPP.9000092

Astuti, I.P., 2011. Studi kasus taksonomi Citrus hystrix DC koleksi Kebun Raya Bogor. Special Topics in Plants and Algae. Berkala Penelitian Hayati, 7A, pp.87 – 89.

Astuti, I.P., & Ajiningrum, P.S., 2019. Citrus hystrix DC. collected from Central Java and East Sumba at the Bogor Botanical Garden. Jurnal Biologi Indonesia, 15(2),pp.199-204. doi: 10.14203/jbi.v15i2.3813

Baccati, C. et al., 2021. Chemical variability of peel and leaf essential oils in the Citrus Subgenus Papeda (Swingle) and few relatives. Plants, 10, 1117. doi: 10.3390/plants10061117

De Santana, M.T. et al., 2013. Citronellal, a monoterpene present in Java citronella oil, attenuates mechanical nociception response in mice. Pharmaceutical Biology, 51(9), pp.1144-1149. doi: 10.3109/13880209.2013.781656

Ebrahimi, S.N. et al., 2010. Essential oil compositions of different accessions of Coriandrum sativum L. From Iran. Natural Product Research, 24(14), pp.1287-1294. doi: 10.1080/14786410903132316

Ikawati, S. Et al., 2017. Bioactivity of Citrus hystrix DC. Leaf against cigarette beetle Lasioderma serricorne (F.). The Journal of Tropical Life Science, 7(3), pp.189-196. doi: 10.11594/jtls.07.03.01

Kamatou, G.P.P., & Viljoen, A., 2008. Linalool – A review of a biologically active compound of commercial importance. Natural product communications, 3(7), pp.1183-1192. doi: 10.1177%2F1934578X0800300727

Kidarn, S. et al., 2018. Furanocoumarins from kaffir lime and their inhibitory effect on inflammatory mediator production. Cogent Chemistry, 4(1), 1529259. doi: 10.1080/23312009.2018.1529259

Lim, T.K., 2012. Edible medicinal and non medicinal plants. Volume 4, Springer.

Loh, F.S. et al., 2011. Insecticidal properties of Citrus hystrix DC leaves essential oil against Spodoptera litura fabricius. Journal of Medicinal Plants Resesearch, 5(16), pp.3739-3744. doi: 10.5897/JMPR.9000813

Mustafa, N.E.M., 2015. Citrus essential oils: current and prospective uses in the food industry. Recent Patents on Food, Nutrition & Agriculture, 7, pp.115-127. doi: 10.2174/2212798407666150831144239

Ngan, T.T.K. et al., 2019. Physico-chemical profile of essential oil of kaffir lime (Citrus hystrix DC) grown in An Giang Province, Vietnam. Asian Journal of Chemistry, 31(12), pp.2855-2858. doi: 10.14233/ajchem.2019.22167

Omar, M.N., 1999. Volatile aroma compounds in Citrus hystrix oil. Journal of Tropical Agriculture and Food Science, 27(2), pp.225-229.

Palazzolo, E. Et al., 2013. Current and potential use of citrus essential oils. Current Organic Chemistry, 17, pp.3042-3049. doi: 10.2174/13852728113179990122

Salehi, B. et al., 2019. Therapeutic potential of α- and β-pinene: a miracle gift of nature. Biomolecules, 9, 738. doi: 10.3390/biom9110738

Soulimani, R. et al., 2019. Limonene: Natural monoterpene volatile compounds of potential therapeutic interest. American Journal of Essential Oils and Natural Products, 7(4), pp.1-10.

Sudiyarmanto, S. et al., 2017. Catalytic conversion of Citronellal to Citronellol over skeletal Ni catalyst. Basic and Applied Sciences Interdisciplinary Conference 2017 IOP Publishing. doi: 10.1088/1742-6596/1442/1/012047

Tanzil, L. et al., 2017. Antidandfruff activity of extracts from kaffir lime (Citrus hystrix DC.) prepared by different solvents. SANITAS Jurnal Teknologi dan Seni Kesehatan, 8(1), pp.57-62. doi: 10.36525/sanitas.2017.9

Tunjung, W.A.S. et al., 2014. Anticancer effect of kaffir lime (Citrus hystrix DC) leaf extract in cervical cancer and neuroblastoma cell lines. Procedia Chemistry, 14, pp.465-468. doi: 10.1016/j.proche.2015.03.062

Tunjung, W.A.S. et al., 2018. Antibacterial activity and composition of crude extracts of kaffir lime (Citrus hystrix DC.) leaves and callus. Proceeding of the Pakistan Academy of Sciences, 55(2).

Warsito et al., 2017. Aktivitas antioksidan dan antimikrobia minyak jeruk purut (Citrus hystrix DC.) dan komponen utamanya. Journal of Enviromental Engineering & Sustainable Technology, 4(1), pp.13-18.

Wikandari, R.J., & Surati, S., 2018. Efek ekstrak kulit jeruk purut (Citrus hystrix DC.) terhadap morfologi dan histologi larva Aedes aegypti. ASPIRATOR, 10(2), pp.119-126. doi: 10.22435/asp.v10i2.193

Yang, L. et al., 2018. Response of plant secondary metabolites to environmental factors. Molecules, 23. doi: 10.3390/molecules23040762


Article Metrics

Abstract views : 3857 | views : 3195


  • There are currently no refbacks.

Copyright (c) 2022 Journal of Tropical Biodiversity and Biotechnology

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Editoral address:

Faculty of Biology, UGM

Jl. Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia

ISSN: 2540-9581 (online)