Using Feathers for Molecular Sexing of Straw-headed Bulbul (Pycnonotus zeylanicus) Offsprings

https://doi.org/10.22146/jtbb.67129

Pramana Yuda(1*), Worawidh Wajjwalku(2)

(1) Faculty of Tehcnobiology, Universitas Atma Jaya Yogyakarta
(2) Akkharatchakumari Veterinary College, Walailak University, Thasala, Nakhonsithammarat
(*) Corresponding Author

Abstract


Sex determination of straw-headed bulbul offspring was carried out from 27 offspring’s plucked feather samples in a captive breeding program. Using direct PCR, this study provided more evidences that feather samples are reliable as a source of DNA for non-invasive and effective molecular sexing. The study also revealed that the offspring sex ratio of straw-headed bulbul was slightly inclined towards males, but there was no significant difference from the value of 0.5.  

 


Keywords


dPCR; feather; molecular sexing; non-invasive sampling; straw-headed Bulbul

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References

Atlas Burung Indonesia, 2020. Atlas Burung Indinesia- wujud karya peneliti amatir dalam memetakan burung Nusantara, Batu: Yayasan Atlas Burung Indonesia.

Bergin, D. et al., 2018. The final straw? An overview of Straw-headed Bulbul Pycnonotus zeylanicus trade in Indonesia. Bird Conservation International, 28, pp.126–132. doi: 10.1017/s0959270917000302.

BirdLife International, 2001. Threatened Birds of Asia: the BirdLife International Red Data Book, Cambridge, UK: BirdLife International.

BirdLife International, 2018. Pycnonotus zeylanicus. The IUCN Red List of Threatened Species 2018: e.T22712603A94338033. doi: 10.2305/IUCN.UK.2016-3.RLTS.T22712603A94338033.en.

Costantini, V. et al., 2008. DNA sexing in Humboldt Penguins (Spheniscus humboldti) from feather samples. Animal Reproduction Science, 106(1–2), pp.162–167. doi: 10.1016/j.anireprosci.2007.12.013.

Donald, P.F., 2007. Adult sex ratios in wild bird population. Ibis, 149, pp.671–692. doi: 10.1111/j.1474-919X.2007.00724.x.

Fishpool, L., Tobias, J. & Sharpe, C.J., 2020. Straw-headed Bulbul (Pycnonotus zeylanicus). In J. del Hoyo et al., eds. Birds of the World. Ithaca, NY, USA: Cornell Lab of Ornithology. doi: 10.2173/bow.sthbul1.01.

Fridolfsson, A. & Ellegren, H., 1999. A simple and universal method for molecular sexing of non-ratite birds. Journal of Avian Biology, 30(1), pp.116–121. doi: 10.2307/3677252.

Griffiths, R. et al., 1998. A DNA test to sex most birds. Molecular Ecology, 7(8), pp.1071–1075. doi: 10.1046/j.1365-294x.1998.00389.x.

Harvey, M.G. et al., 2006. A comparison of plucked feathers versus blood samples as DNA sources for molecular sexing. J. Field Ornithol., 77(22), pp.136–140. doi: 10.1111/j.1557-9263.2006.00033.x.

Hidayat, R.F.K. et al., 2021. Molecular bird sexing of Tanimbar cockatoos (Cacatua goffiniana) by using polymerase chain reaction method. Journal of Tropical Biodiversity and Biotechnology, 6(2). doi: 10.22146/JTBB.59997.

Horváth, M.B. et al., 2005. An overlooked DNA source for non-invasive genetic analysis in birds. Journal of Avian Biology, 36(1), pp.84–88. doi: 10.1111/j.0908-8857.2005.03370.x.

Kahn, N.W., John, J.S.T. & Quinn, T.W., 1998. Chromosome-specificIntron size differences in the avian CHD gene provide an efficient method for sex identification in birds. The Auk, 115(4), pp.1074–1078. doi: 10.2307/4089527.

Lee, J.C.-I.I. et al., 2010. A novel strategy for avian species and gender identification using the CHD gene. Molecular and Cellular Probes, 24(1), pp.27–31. doi: 10.1016/j.mcp.2009.08.003.

Lestari, D.A., Masy’ud, B. & Hernowo, J.B., 2015. Management of captive breeding and song quality Of Straw- headed bulbul ( Pycnonotus zeylanicus Gmelin , 1789 ) in Mega Bird and Orchid Farm, Bogor. Media Konservasi, 20(2), pp.132–141.

Miura, M. et al., 2013. Comparason of six commercially-available DNA polymerases for direct PCR. Revista do Instituto de Medicina Tropical de Sao Paulo, 55(6), pp.401–406. doi: 10.1590/S0036-46652013000600005.

Pamulang, Y.V. & Haryanto, A., 2021. Molecular bird sexing on kutilang (Pycnonotus sp.) based on amplification of CHD-Z and CHD-W genes by using polymerase chain reaction method. Biodiversitas, 22(1), pp.449–452. doi: 10.13057/biodiv/d220155.

Pratomo, Y.W., Zahida, F. & Yuda, P., 2021. Perbandingan metode isolasi DNA sebagai templat PCR untuk identifikasi jenis kelamin Cerek jawa ( Charadriius javanicus ) secara molekuler menggunakan rimer 2550F / 2718R. Biota, 6(2), pp.78–86. doi: 10.24002/biota.v6i2.3444.

Quintana, F., Lopez, G.C. & Somoza, G., 2008. A cheap and quick method for DNA-based sexing of Birds. Waterbirds, 31(3), pp.485–488. doi: 10.1675/1524-4695-31.3.485.

Redelman, D., Fleury, S.A. & Garner, D.L., 1997. Flow cytometry for sexing birds. Trends in Ecology & Evolution, 12(12), p.489. doi: 10.1016/S0169-5347(97)84408-4.

Richner, H., 1989. Avian laparoscopy as a field technique for sexing birds and an assessment of its effect on wild birds. J. Field Ornithol., 60(2), pp.137–142.

Saputra, A.W. & Yuda, P., 2020. Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia. Biodiversitas, 21(10), pp.4772–4777. doi: 10.13057/biodiv/d211042.

Segelbacher, G., 2002. Noninvasive genetic analysis in birds: testing reliability of feather samples. Molecular Ecology Notes, 2(3), pp.367–369. doi: 10.1046/j.1471-8286.2002.00180.x.

Smith, T.B. et al., 2003. A call for feather sampling. Auk, 120, pp.218–221. doi: 10.1642/0004-8038(2003)120[0218:ACFFS]2.0.CO;2.

Sulandari, S. & Zein, M.S.A., 2012. Application of two molecular sexing methods for Indonesian bird species: Implication for captive breeding programs in Indonesia. HAYATI, 19(4), pp.183–190.

Ushine, N., Hayama, S. & Kato, T., 2016. Sex identification in Japanese birds using dropping as a source of DNA. Bulletin of the Japanese Bird Banding Association, 28, pp.51–70.

Vili, N. et al., 2013. Factors affecting DNA quality in feathers used for non-invasive sampling. Journal of Ornithology, 154(2), pp.587–595. doi: 10.1007/s10336-013-0932-9.

Wirastika, P.I.P., Yuda, I.P. & Zahida, F., 2015. Sex Determination of Bali Starling (Leucopsar rothschildi) Using Molecular Sexing. KnE Life Sciences, 2(1), p.114. doi: 10.18502/kls.v2i1.128.

Yuda, P., 2008. Conservation genetics of the Java sparrow (Padda oryzivora) and an analysis of its viability. Cairns: James Cook University.

Yuda, P., Kinanti, R. & Saputra, A.W., 2020. Use of swab for DNA sampling from confiscated raptors for molecular sexing. IOP Conference Series: Earth and Environmental Science, 590, pp.1–6. doi: 10.1088/1755-1315/590/1/012011.

Yuda, P. & Saputra, A.W., 2021. Eggshell membrane for DNA sexing of the endangered Maleo (Macrocephalon maleo). F1000Research, 9, p.599. doi: 10.12688/f1000research.23712.4.



DOI: https://doi.org/10.22146/jtbb.67129

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