Scanning Electron Microscopy Analysis of Tea’s Embryo Axis Explant Cultured on Murashige and Skoog Medium Containing 2,4-Dichlorophenoxyacetic acid

https://doi.org/10.22146/jtbb.76451

Ratna Dewi Eskundari(1*), Taryono Taryono(2), Didik Indradewa(3), Yekti Asih Purwestri(4)

(1) Department of Biotechnology, Graduate School of Universitas Gadjah Mada. Yogyakarta 55281, Indonesia; Biology Education Study Program, Faculty of Teacher Training and Education, Universitas Veteran Bangun Nusantara, Sukoharjo, 57521, Central Java, Indonesia.
(2) Department of Biotechnology, Graduate School of Universitas Gadjah Mada. Yogyakarta 55281, Indonesia; Department of Agriculture, Faculty of Agriculture, Universitas Gadjah Mada. Yogyakarta 55281, Indonesia; Agrotechnology Innovation Centre, Universitas Gadjah Mada. Yogyakarta, Indonesia
(3) Department of Agriculture, Faculty of Agriculture, Universitas Gadjah Mada. Yogyakarta 55281, Indonesia.
(4) Department of Biotechnology, Graduate School of Universitas Gadjah Mada. Yogyakarta 55281, Indonesia; Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada. Yogyakarta 55281, Indonesia.
(*) Corresponding Author

Abstract


Camellia sinensis L. is an important crop in Indonesia as healthy beverage that contains several secondary metabolism compounds, such as polyphenols and catechins. Tissue culture including somatic embryogenesis and organogenesis has been used for propagating plant for various needs. In this present short-communication, scanning electron microscopic (SEM) analysis of tea was conducted and discussed. This study aimed to investigate surface ultrastructure of TRI2025 embryo axis tea clone cultured on Murashige and Skoog (MS) medium containing 2,4-Dichlorophenoxyacetic acid (2,4-D). The results revealed two different forms of explant’s development, i.e. somatic embryo and transitional form between somatic embryogenesis and organogenesis; or called by “Globular-like Structure” (GLS). Surface ultrastructure analysis of somatic embryo and GLS revealed respectively many stages of somatic embryo development i.e. globular, torpedo, and cotyledon stage, and leaf development form GLS regeneration.

 


Keywords


Camellia sinensis; somatic embryo; tea; ultrastructure

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DOI: https://doi.org/10.22146/jtbb.76451

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