Stomata characters of sugarcane (Saccharum officinarum L.) mutants of GMP3 variety at PT Gunung Madu Plantations, Lampung, Indonesia

https://doi.org/10.22146/jtbb.79860

Mahfut Mahfut(1*), Putri Kendari(2), Admi Syarif(3), Sri Wahyuningsih(4), Endah Susiyanti(5)

(1) Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung. Jl. Prof., Dr. Ir. Soemantri Brodjonegoro, No. 1. Gedong Meneng, Kec. Rajabasa, Bandar Lampung, Lampung, 35145
(2) Postgraduate Student of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung. Jl. Prof., Dr. Ir. Soemantri Brodjonegoro, No. 1. Gedong Meneng, Kec. Rajabasa, Bandar Lampung, Lampung, 35145
(3) Department of Computer Science, Faculty of Mathematics and Natural Sciences, University of Lampung. Jl. Prof., Dr. Ir. Soemantri Brodjonegoro, No. 1. Gedong Meneng, Kec. Rajabasa, Bandar Lampung, Lampung, 35145
(4) Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung. Jl. Prof., Dr. Ir. Soemantri Brodjonegoro, No. 1. Gedong Meneng, Kec. Rajabasa, Bandar Lampung, Lampung, 35145
(5) Division of Agronomy, Research and Development, PT. Gunung Madu Plantations. KM 90 Terbanggi Besar, Gunung Batin Udik, Terusan Nunyai, Lampung, 34167
(*) Corresponding Author

Abstract


The induction of colchicine mutations is one method of breeding. PT Gunung Madu Plantations, for example, has induced mutations of commercial sugarcane (Saccharum officinarum L.) varieties, however, investigations on the impact of colchicine on stomatal characters have received less attention. Therefore, this study aimed to analyse the stomata character of 21 sugarcane mutants of the GMP3 variety at PT Gunung Madu Plantations, Lampung, Indonesia with a focused look at stomata aperture width, stomata length and width, number of stomata, stomatal density, and stomata index. The collected data were analysed using cluster and Principal Component Analysis (PCA) through MVSP software. This study showed that all GMP3 mutants had Graminae-type stomata. In terms of stomata length and width, the average size of the GMP3 variety mutant was greater than that of the control. The diversity of stomata characters is fairly high due to differences in stomata size between GMP3 and control mutants. With a similarity index of 0.20, the phenetic analysis of 21 mutants of the GMP3 variety revealed that the relationship between mutants and controls was getting further. A six-character principal component analysis revealed that axis I's total variation accounted for 40.54 percent of the variation and had an eigenvalue of 2.43, whereas axis II's contribution to the variation was 19.02 percent and had an eigenvalue of 1.14. The findings indicate that stomata are excellent taxonomic evidence for identifying and analysing sugarcane varieties induced by colchicine-induced breeding.

 


Keywords


Colchicine; GMP3; Indonesia; Saccharum officinarum L.; Stomata; Sugarcane

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References

Adebisi, M.A. et al., 2013. Evaluation of variation in seed vigor character of the West African rice genotype (Oryza sativa L.) using multivariate technique. Am J Plant Sci., 4, pp.356-363. doi: 10.4236/ajps.2013.42047.

Arofatun, I.N., Rugayah, R. & Chikmawati, T., 2020. Leaf stomata variation in Desmos Lour. and Dasymaschalon (Hook. F. & Thomson) Dalla Torre & Harms species (Annonaceae). Biodiversitas, 21(7), pp.3317-3330. doi: 10.13057/biodiv/d210756.

Arzani, K. et al., 2013. Study of foliar epidermal anatomy of four pistachio rootstocks under water stress. Idesia, 31(1), pp.101-107. doi: 10.4067/S0718-34292013000100012.

Asif, A. & Khalil, A.M.Y., 2019. Generation of mutant lines of Nigella sativa L. by induced mutagenesis for improved seed yield. Industrial Crops and Products, 13(9), pp.111-252. doi: https://doi.org/10.1016/j.indcrop.2019.11.

Bagheri, M. & Mansouri, H., 2014. Effect of induced polyploidy on some biochemical parameters in Cannabis sativa L. J Biotechnol Appl Biochem., 175(5), pp.2366-2375. doi: 10.1007/s12010-014-1435-8.

Carsono, N. et al., 2022. Agronomic characteristics and genetic relationship of putative transgenic rice lines of cv. Fatmawati with the Glu-IDx5 Transgene. Biodiversitas, 23(1), pp.291-298. doi:10.13057/biodiv/d230135.

Central Bureau of Statistics, 2021. Indonesian Sugarcane Statistics. Central Bureau of Statistics.

Chikmawati, T., 2013. Stomata and cytological features of Spatthoglottis plicata from Java Island. J Trop Life Sci., 3(2), pp.87-92. doi: 10.11594/JTLS.03.02.03.

Gantait, S., Mandal, N. & Bhattacharyya, S., 2011. Induction and identification of tetraploids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella. Plant Cell Tissue Organ Cult., 106(2), pp.485–493. doi: 10.1186/s43141-021-00269-1.

Hamidah, H., Tsawab & Rosmanida, 2016. Analysis of Hylocereus spp. diversity based on phonetic methods. AIP Conference Proceedings, 1854. doi:10.1063/1.4985403.

Hanafy, R.S. & Akladious, S.A., 2018. Physiological and molecular studies on the effect of gamma radiation in fenugreek (Trigonella foenumgraecum L.) plants. J Genet Eng Biotechnol., 16(2), pp.683-692. doi: https://doi.org/10.1016/j.jgeb.2018.02.01 2.

Jalil, M. et al., 2020. Distribution, variation, and relationship of Curcuma soloensis Valeton in Java, Indonesia based on morphological caharacters. Biodiversitas, 21(8), pp.3867-3877. doi I:10.13057/biodiv/d210856.

Kamwean, P. et al., 2017. Chaging of morphological characteristic and biomass properties in Pennisetum grassland soil. Soil Sci Soc AmJ., 68, pp.1429-1436. doi:10.3923/JA.2017.23.31.

Lubis, M.M., Mawarni, L. & Husni, Y., 2015. Response to growth of Sugarcane (Saccharum officinarum L.) against tillage on two conditions of drainage. Jurnal Agroekoteknologi, 3(1), pp.214-220. doi: 10.32734/jaet.v3i1.9385.

Mahfut et al., 2021. Identification of Dendrobium (Orchidaceae) in Liwa Botanical Garden based on leaf morphological characters. Journal of Tropical Biodiversity and Biotechnology, 6(1), pp.1-6. doi: 10.22146/jtbb.59423.

Mahfut, Hidayat, M.M. & Arifannisa, S.J., 2023. Study of orchid resistance induction using Rhizoctonia against ORSV infection based on anatomical characters of roots and leaves. Asian Journal of Plant Sciences, 22(2), pp.239-249. doi: 10.3923/ajps.2023.239.249.

Miguel, T.P. & Leonhart, K.W., 2011. In vitro polyploid induction of orchids using oryzalin. Scie Horror., 130, pp.314-319. doi: 10.1016/J.scienta.2011.07.002.

Moghbei, N., Khalili, M.B. & Bernard, F., 2015. Colchicine effect on the DNA content and stomata size of Glycyrrhiza glabra var. glandulifera and Carthamus tinctorius L. J Genet Eng Biotechnol., 13(1), pp.1-6. doi: 10.1016/j.jgeb.2016.02.002.

Mugiono, 2010. Plant Breeding With Mutation Techniques. Isotope and Radiation Technology Research Center. Jakarta.

Munir, M. et al., 2011. Foliar epidermal anatomy of some ethnobotanically important species of wild edible fruits of northern Pakistan. J Med Plant Res., 5(24), pp.5873-5880.

Pitoyo, A. et al., 2018. Morphological, stomata, and isozyme variability among taro (Colocasia esculenta) accessions from the southeastern part of Central Java, Indonesia. Biodiversitas, 19(5), pp.1811-1819. doi: 10.13057/biodiv/d190532.

Prabowo, H. et al., 2022. Stable isotope analysis to assess the trophic level of arthropod in sugarcane ratoon agroecosystem. Biodiversitas, 23(6), pp.2871-2881. doi: 10.13057/biodiv/d230613.

PT Gunung Madu Plantations, 2016. Company history. Gunung Madu Press.

Purnomo et al., 2012. Phenetic analysis and intraspecific classification of Indonesian water yam (Dioscorea alata L.) germplasm based on morphological characters. SABRAO J Breed Genet., 44(2), pp.277-291.

Purnomo et al., 2020. Phenetic analysis of cultivated taro (Colocasia esculenta L. Schott) accessions based on morphological characters. SABRAO J Breed Genet., 52(3), pp.231-247.

Rohmah, A. et.al., 2017. Influence of Colchicine Present toward Stomata Characters of Oliv Leaf (Olea europaea L.). Jurnal Ilmiah Bioscience Tropic, 2(2), pp.10-17.doi:10.33474/e-jbst.v2i2.81.

Sari, N. et al., 2016. Variation and intraspecies classification of edible Canna (Canna indica L.) based on morphological characters. AIP Conference Proceedings, 1744. doi: 10.1063/1.49535155.

Sivakumar, G., 2018. Upstream biomanufacturing of pharmaceutical colchicine. Critical Review in Biotechnology, 38(1), pp.83-92. doi: 10.1080/07388551.2017.1312269.

Stevens, R.D. & Tello, J.S., 2014. On the measurement of dimensionality of biodiversity. Glob Ecol Biogeogr., 23(2), pp.1115-1125. doi: 10.1111/geb.12192.

Suhaimi, S., 2017. Evaluation of Morphology, Anatomy, Physiology and Anatomy of Forage Plants Obtained by Colchiisin Treatment. Universitas Diponegoro.

Sultan, H.A. et. al., 2010. Stomata and phytochemical studies of the leaves and roots of Urginea grandiflora Bak. and Pancratium tortuosum Herbert. Ethnobot Leafl., 14, pp.826-835. doi: 10.1.1.1.683.9435.

Suryo, H., 2009. Cytogenetics, Gadjah Mada University Press.

Syukur, M. et.al., 2015. Plant Breeding Techniques. Jakarta: Penebar Swadaya.

Tambaru, E., 2015. Identification of morphological and anatomical characteristics Flacourtia inermis Roxb. in the Unhas Tamalanrea Makassar campus area. Journal of Natural and Environmental Science, 6(11), pp. 35-41.

Windiyani, I.P. et al., 2022. Morphological variations of superior sugarcane cultivars (Saccharum officinarum L.) from Lampung, Indonesia. Biodiversitas, 23(8), pp.4109-4116. doi: 10.13057/biodiv/d230831.

Yanny, D.L. et. al., 2022. Increasing the diversity of marigold (Tagetes sp.) by acute and chronic chemical induced mutation of EMS (Ethyl Methane Sulfonate). J Biodiversitas. 23(3):1399-1407. doi:10.13057/biodiv/d230326.



DOI: https://doi.org/10.22146/jtbb.79860

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