* Corresponding Author
Characterization of Lactic Acid Bacteria Isolated from Soymilk and Its Growth in Soymilk By-product Medium for the Application in Soymilk Fermentation
https://doi.org/10.22146/jtbb.89003 Faizah Diah Retnowati(1), Yekti Asih Purwestri(2*), Yuni Sine(3)
(1) Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Sendowo, Sinduadi, Kec. Mlati, Kabupaten Sleman, Daerah Istimewa Yogyakarta 55281
(2) Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Sendowo, Sinduadi, Kec. Mlati, Kabupaten Sleman, Daerah Istimewa Yogyakarta 55281
(3) Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Sendowo, Sinduadi, Kec. Mlati, Kabupaten Sleman, Daerah Istimewa Yogyakarta 55281
(*) Corresponding Author
Abstract
The separation phase of soy-product manufacturing, especially of soymilk production, involves using the liquid portion of soy while disposing primarily of the solid portion, known as soymilk by-product (SMB). The improper disposal of SMB can contribute to environmental concerns. As SMB contains many beneficial nutrients, this could serve as a valuable culture medium for lactic acid bacteria (LAB), especially considering the expense of the standard de Man, Rogosa, and Sharpe (MRS) medium. This study aimed to isolate and identify LAB from soymilk through 16S rRNA sequencing, assess the potential of SMB as a culture medium for LAB, and ferment soymilk with LAB cultured from SMB to demonstrate the probiotic capacity. The research identified a potential LAB as Weissella confusa. Effective cultivation of LAB was demonstrated at 2% SMB concentration, although LAB cultured with MRS medium yielded a higher colony count. Furthermore, fermentation of soymilk by the LAB isolates from SMB exhibited a positive probiotic ability, reaching 5.5 × 109 CFU/mL, with a lactic acid content of 0.27%.
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Abdul Malik, N.H., Simarani, K. & Aziz, M.A., 2022. SOYBEAN AS AN ALTERNATIVE NUTRIENT MEDIUM FOR Bacillus subtilis GROWTH. Malaysian Applied Biology, 51(4), pp.67–74. doi: 10.55230/mabjournal.v51i4.12.
Alp, D. & Bulantekin, Ö., 2021. The microbiological quality of various foods dried by applying different drying methods: a review. European Food Research and Technology, 247(6), pp.1333–1343. doi: 10.1007/s00217-021-03731-z.
Aritonang, S.N. et al., 2017. Isolation and Identification of Lactic Acid Bacteria from Okara and Evaluation of Their Potential as Candidate Probiotics. Pakistan Journal of Nutrition, 16(8), pp.618–628. doi: 10.3923/pjn.2017.618.628.
Aydar, E.F., Tutuncu, S. & Ozcelik, B., 2020. Plant-based milk substitutes: Bioactive compounds, conventional and novel processes, bioavailability studies, and health effects. Journal of Functional Foods, 70, p.103975. doi: 10.1016/j.jff.2020.103975.
Ayeni, F.A. et al., 2011. Evaluation of the functional potential of Weissella and Lactobacillus isolates obtained from Nigerian traditional fermented foods and cow’s intestine. International Journal of Food Microbiology, 147(2), pp.97–104. doi: 10.1016/j.ijfoodmicro.2011.03.014.
Ayo-Omogie, H. & Okorie, E., 2016. In vitro Probiotic Potential of Autochthonous Lactic Acid Bacteria and Microbiology of Kunu Made from Mixed Grains. British Microbiology Research Journal, 14(4), pp.1–10. doi: 10.9734/BMRJ/2016/25403.
Bayu, H.H. et al., 2023. Isolation and Identification of Lactic Acid Bacteria from Channa sp. as Potential Probiotic. JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS, 9(1), pp.75–84. doi: 10.36987/jpbn.v9i1.3551.
Chun, J. et al., 2007. Conversion of Isoflavone Glucosides to Aglycones in Soymilk by Fermentation with Lactic Acid Bacteria. Journal of Food Science, 72(2). doi: 10.1111/j.1750-3841.2007.00276.x.
Davy, P. & Vuong, Q.V., 2022. Soy Milk By-product: Its Composition and Utilisation. Food Reviews International, 38(sup1), pp.147–169. doi: 10.1080/87559129.2020.1855191.
Di Cagno, R. et al., 2007. Characterization of Italian Cheeses Ripened Under Nonconventional Conditions. Journal of Dairy Science, 90(6), pp.2689–2704. doi: 10.3168/jds.2006-654.
Elbanna, K. et al., 2018. In vitro and in vivo evidences for innate immune stimulators lactic acid bacterial starters isolated from fermented camel dairy products. Scientific Reports, 8(1), p.12553. doi: 10.1038/s41598-018-31006-3.
Fairfax, M.R., Lephart, P.R. & Salimnia, H., 2014. Weissella confusa: problems with identification of an opportunistic pathogen that has been found in fermented foods and proposed as a probiotic. Frontiers in Microbiology, 5. doi: 10.3389/fmicb.2014.00254.
Fessard, A. & Remize, F., 2017. Why Are Weissella spp. Not Used as Commercial Starter Cultures for Food Fermentation? Fermentation, 3(3), p.38. doi: 10.3390/fermentation3030038.
Goda, H., Barakat, O. & Ali, M., 2011. Potential Exploitation of Okara as an Alternative Medium for Edible Pleurotus ostreatus Mycelium Production. Arab Journal of Biotechnology, 14, pp.139–153.
Gunawan, S. et al., 2021. Reaction kinetics of lactic acid fermentation from bitter cassava (Manihot glaziovii) starch by Lactobacillus casei. Indonesian Journal of Biotechnology, 26(1), p.7. doi: 10.22146/ijbiotech.54119.
Hayek, S.A. et al., 2019. Cultivation media for lactic acid bacteria used in dairy products. Journal of Dairy Research, 86(4), pp.490–502. doi: 10.1017/S002202991900075X.
Ismail, Y., Yulvizar, C. & Mazhitov, B., 2018. Characterization of lactic acid bacteria from local cow´s milk kefir. IOP Conference Series: Earth and Environmental Science, 130, p.012019. doi: 10.1088/1755-1315/130/1/012019.
Karyantina, M. et al., 2020. Moderate Halophilic Lactic Acid Bacteria from Jambal roti : A Traditional Fermented Fish of Central Java, Indonesia. Journal of Aquatic Food Product Technology, 29(10), pp.990–1000. doi: 10.1080/10498850.2020.1827112.
Kim, J., 2019. Transforming okara into a microalgae culture medium. Nanyang Technological University. doi: 10.32657/10220/49477.
Liu, Y. et al., 2016. Characterization of Lactobacillus pentosus as a starter culture for the fermentation of edible oyster mushrooms (Pleurotus spp.). LWT - Food Science and Technology, 68, pp.21–26. doi: 10.1016/j.lwt.2015.12.008.
Malik, T.F. & Panuganti, K.K., 2022. Lactose Intolerance. In StatPearls. Treasure Island (FL): StatPearls Publishing. Available at: http://www.ncbi.nlm.nih.gov/books/NBK532285/ [Accessed February 18, 2024].
Oktari, A. et al., 2017. The Bacterial Endospore Stain on Schaeffer Fulton using Variation of Methylene Blue Solution. Journal of Physics: Conference Series, 812, p.012066. doi: 10.1088/1742-6596/812/1/012066.
Parseelan, A. et al., 2019. Aerobic Plate Count of Milk and Dairy Products Marketed in Different Zones of Chennai. International Journal of Livestock Research, (0), p.1. doi: 10.5455/ijlr.20180711104721.
Rahayu, H.M. & Setiadi, A.E., 2023. Isolation and Characterization of Indigenous Lactic Acid Bacteria from Pakatikng Rape, Dayak’s Traditional Fermented Food. Jurnal Penelitian Pendidikan IPA, 9(2), pp.920–925. doi: 10.29303/jppipa.v9i2.2801.
Rizzo, G. & Baroni, L., 2018. Soy, Soy Foods and Their Role in Vegetarian Diets. Nutrients, 10(1), p.43. doi: 10.3390/nu10010043.
Sanders, E.R., 2012. Aseptic Laboratory Techniques: Plating Methods. Journal of Visualized Experiments, (63), p.3064. doi: 10.3791/3064.
Spiegelhauer, M.R. et al., 2020. A case report of polymicrobial bacteremia with Weissella confusa and comparison of previous treatment for successful recovery with a review of the literature. Access Microbiology, 2(5). doi: 10.1099/acmi.0.000119.
Teh, S. et al., 2010. Enhanced Growth of Lactobacilli in Soymilk upon Immobilization on Agrowastes. Journal of Food Science, 75(3). doi: 10.1111/j.1750-3841.2010.01538.x.
Tripathi, N. & Sapra, A., 2023. Gram Staining. In StatPearls. Treasure Island (FL): StatPearls Publishing. Available at: http://www.ncbi.nlm.nih.gov/books/NBK562156/ [Accessed February 18, 2024].
Tyl, C. & Sadler, G.D., 2017. pH and Titratable Acidity. In S. S. Nielsen, ed. Food Analysis. Food Science Text Series. Cham: Springer International Publishing, pp. 389–406. doi: 10.1007/978-3-319-45776-5_22.
Utomo, A.S., Harismah, K. & Mulyaningtyas, A., 2022. Making Bacillus cereus Bacterial Growth Media from Soybeans (Glycine max (L.) Merril): In 4th International Conference Current Breakthrough in Pharmacy (ICB-Pharma 2022). Sukoharjo, Indonesia. doi: 10.2991/978-94-6463-050-3_26.
Vanga, S.K. & Raghavan, V., 2018. How well do plant based alternatives fare nutritionally compared to cow’s milk? Journal of Food Science and Technology, 55(1), pp.10–20. doi: 10.1007/s13197-017-2915-y.
Yang, J. et al., 2014. Selection of functional lactic acid bacteria as starter cultures for the fermentation of Korean leek (Allium tuberosum Rottler ex Sprengel.). International Journal of Food Microbiology, 191, pp.164–171. doi: 10.1016/j.ijfoodmicro.2014.09.016.
Yudianti, N.F. et al., 2020. Isolation and Characterization of Lactic Acid Bacteria from Legume Soaking Water of Tempeh Productions. Digital Press Life Sciences, 2, p.00003. doi: 10.29037/digitalpress.22328.
DOI: https://doi.org/10.22146/jtbb.89003
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