* Corresponding Author
Utilisation of Snails for Wound Healing: A Review
https://doi.org/10.22146/jtbb.90236 Diana Fadhilah(1), Putra Santoso(2), Rita Maliza(3*)
(1) Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University, Limau Manis, Padang 25163, West Sumatra, Indonesia
(2) Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University, Limau Manis, Padang 25163, West Sumatra, Indonesia
(3) Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University, Limau Manis, Padang 25163, West Sumatra, Indonesia
(*) Corresponding Author
Abstract
Snails exhibit remarkable adaptability, allowing them to flourish in diverse environmental conditions and resulting in thriving populations in specific regions. This abundance has led communities to harness snails for various purposes, including their use as animal feed, daily dietary source, and in traditional wound-healing practices with historical roots. The primary objective of this systematic review is to identify the snail species commonly employed in wound healing and evaluate the bioactivity of compounds derived from different snail species. This review was conducted using literature review method, drawing from international databases such as Scopus, and encompassed publications from 2013 to 2023. A total of 22 articles met the inclusion and exclusion criteria. Snail body parts that have been explored for wound-healing purposes include both the body and the shell, along with snail secretions, particularly their mucus. Various methods have been employed to extract mucus, involving manual stimulation of the snail's body, spraying with a saline solution (NaCl), application of electric shock, and the use of ozone gas through nebulisation. Prominent snail species found to be beneficial for wound healing include Achatina fulica, Helix aspersa, Eobania desertorum, Helix lucurus, Cornu bistrialis, Theba pisana, and Megalobulimus lopesi. These snail species demonstrate potential applications in the treatment of burns, excision wounds, incision wounds, and diabetic ulcers. Key compounds within snail secretions encompass mucopolysaccharides, polyphenols, peptides, and glycosaminoglycans. These compounds exert significant effects on haemostasis, inflammation control, cellular proliferation, and re-epithelialisation, significantly contributing to the wound healing process.
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Agustina, L., Shoviantari, F. &Aditya, D., 2020. Stability test of glycosaminoglycan and achasin in snail (Achatina fullica) slime and its gel formulation. International Journal of Drug Delivery Technology, 10(1), pp.1 – 7. doi: 10.25258/ijddt.10.1.2
Andrade, P.H.M. et al., 2018. Effect of powdered shells treatment of the snail Megalobulimus lopesi on wounds of diabetic rats. Acta Cir Bras, 33(2), pp.185 – 196. doi: 10.1590/s0102-865020180020000010
Benkendorff, K. et al., 2015. Are the traditional medical uses of muricidae molluscs substantiated by their pharmacological properties and bioactive compounds? Mar Drugs, 13(8), pp.5237 – 5275. doi: 10.3390/md13085237
Bowler, P.G., Duerden, B.I. & Armstrong, D.G., 2001. Wound microbiology and associated approaches to wound management. Clin. Microbiol. Rev, 14(2), pp.244 – 269. doi: 10.1128/cmr.14.2.244-269.2001
Deng, T. et al., 2023. A natural biological adhesive from snail mucus for wound repair. Nature communications, 14(396), pp.1 – 18. doi: 10.1038/s41467-023-35907-4
El-Attar, A.A. et al., 2022. Silver/snail mucous pva nanofibers: electrospun synthesis and antibacterial and wound healing activities. Membranes, 12(536), pp.1 – 22. doi: 10.3390/membranes12050536
El-Zawawy, N.A. & Mona, M.M., 2021. Antimicrobial efficacy of Egyptian Eremina desertorum and Helix aspersa snail mucus with a novel approach to their anti‑inflammatory and wound healing potencies. Scientific Reports, 11(24317), pp.1 – 11. doi: 10.1038/s41598-021-03664-3
Fadillah, M. & Santoso, P., 2019. The sirangak (Cyanthillium cinereum; Asteraceae) oil accelerates sliced-wound healing by enhancing the hematological endurance in male albino mice. IOP Conf. Series: Journal of Physics: Conf. Series, 1317, pp.1 – 8. doi: 10.1088/1742-6596/1317/1/012080
Fleck, C.A. & Simman, R., 2010. Modern collagen wound dressings: Function and purpose. J. Am. Coll. Certif. Wound Spec, 2(3), pp.50 – 54. doi: 10.1016/j.jcws.2010.12.003
Gentili, V. et al., 2020. HelixComplex snail mucus as a potential technology against O3 induced skin damage. PLOS ONE, 15(2), pp.1 – 13. doi: 10.1371/journal.pone.0229613
Goyal, R. et al., 2016. Nanoparticles and nanofibers for topical drug delivery. Journal Control, 240, pp.77–92. doi: 10.1016/j.jconrel.2015.10.049
González-Lamothe, R. et al., 2009. Plant antimicrobial agents and their effects on plant and human pathogens. Int J Mol Sci, 10(8), pp.3400 – 3419. doi: 10.3390%2Fijms10083400
Greistorfer, S. et al., 2017. Snail mucus - glandular origin and composition in Helix pomatia. Zoology (Jena), 122, pp.126 – 138. doi: 10.1016/j.zool.2017.05.001
Gubitosa, J. et al., 2020. Biomolecules from snail mucus (Helix aspersa) conjugated gold nanoparticles, exhibiting potential wound healing and anti-inflammatory activity. Soft Matter, pp.1 – 13. doi: 10.1039/d0sm01638a
Gugliandolo, E. et al., 2021. The protective effect of snail secretion filtrate in an experimental model of excisional wounds in mice. Vet. Sci, 8 (167), pp.1 – 11. doi: 10.3390/vetsci8080167
Gugliandolo, E. et al., 2021. Protective effect of snail secretion filtrate against ethanol-induced gastric ulcer in mice. Sci. Rep, 11 (3638), pp.1 – 12. doi: 10.1038/s41598-021-83170-8
Harti, A.S. et al., 2018. The effectiveness of snail mucus (Achatina fulica) and chitosan toward limfosit proliferation in vitro. Asian Journal of Pharmaceutical and Clinical Research, 11(3), pp.85 – 88. doi: 10.22159/ajpcr.2018.v11s3.30041
Igaap, S., Sumerti, N.N. & Nuratni, N.K., 2023. Cytotoxicity test of active compounds natural ingredients of snail mucus (Achatina fulica) against bhk-21 fibroblast cells. Biomedical & Pharmacology Journal, 16(1), pp.371 – 387. doi: 10.13005/bpj/2619
Joe, B., Vijaykumar, M. & Lokesh, B.R., 2004. Biological properties of curcumin cellular and molecular mechanisms of action. Critical Rev Food Sci Nut, 44(2), pp.97 – 112. doi: 10.1080/10408690490424702
Joo, E.J et al., 2005. Nucleolin: acharan sulfatebinding protein on the surface of cancer cells. Glycobiology, 15(1), pp.1–9. doi: 10.1093/glycob/cwh132
Kehinde, A.S. et al., 2020. Biochemical evaluation of meat and haemolymph of African Land Snail (Archachatinamarginata, Swainson) in South-West Nigeria. Egyptian J. Anim. Prod, 57 (3), pp.121 – 126. doi: 10.21608/ejap.2020.121422
Mencucci, R. et al., 2021. Glicopro, novel standardized and sterile snail mucus extract for multi-modulative ocular formulations: new perspective in dry eye disease management. Pharmaceutics, 13(2139), pp.1 – 15. doi: 10.3390/pharmaceutics13122139
Nantarat, N., Tragoolpua, Y. & Gunama, P., 2019. Antibacterial activity of the mucus extract from the giant African snail (Lissachatina fulica) and golden apple snail (Pomacea canaliculata) against pathogenic bacteria causing skin diseases. Tropical Natural History, 19(2), pp.103 – 112. https://li01.tci-thaijo.org/index.php/tnh/article/view/150872
Newar, J. & Ghatak, A., 2015. Studies on the adhesive property of snail adhesive mucus. Langmuir, 31, pp.12155 – 12160. doi: 10.1021/acs.langmuir.5b03498
Nworah, F.N. et al., 2022. Gastroprotective effect of aqueous Achatina l. (snail) slime extract on indomethacin- and acidified ethanol-induced ulceration in wistar albino rats. Pakistan Veterinary Jurnal, 42(4), pp.571 – 575. doi: 10.29261/pakvetj%2F2022.071
Pawar, D.P & Shamkuwar, P.B., 2023. Determination of wound healing potential of pharmaceutical formulations (gel and paste) prepared by using sea water snail. Ind. J. Pharm. Edu. Res, 57(2), pp.419 – 423. doi: 10.5530/ijper.57.2s.49
Putri, D.N., Berniyanti, T. & Jularso, E., 2020. Distribution of Snail Mucous Extract (Achatina Fulica) on the Number of Wound’s Basal Epithelial Cells in Rats of Wistar Strain. Indian Journal of Forensic Medicine & Toxicology, 14(4), pp.920 – 926. doi: 10.37506/ijfmt.v14i4.11611
Ragi, A.S., Leena, P.P. & Nair, S.M., 2016. Study of lipids and amino acid composition of marine gastropod, Tibia curta collected from the southwest coast of India. World Journal of Pharmacy and Pharmaceutical Sciences, 5(3), pp.1058 – 1076.
Rajakaruna, N., Harris, C.S. & Towers, G.H.N., 2002. Antimicrobial activity of plants collected from serpentine outcrops in Sri Lanka. Pharmaceutical Biology, 40 (03), pp.235 – 244. doi: 10.1076/phbi.40.3.235.5825
Refiani, E. et al., 2021. Efek Terapeutik Tanaman Obat pada Penyembuhan Ulkus Diabetikum: Tinjauan Sistematis. Journal of Agromedicine and Medical Sciences, 7(3), pp.167 – 176. doi: 10.19184/ams.v7i3.24244
Song, Y. et al., 2021. Wound-healing activity of glycoproteins from white jade snail (Achatina fulica) on experimentally burned mice. International Journal of Biological Macromolecules, 175, pp.313 – 321. doi: 10.1016/j.ijbiomac.2021.01.193
Trapella, C. et al., 2018. HelixComplex snail mucus exhibits pro-survival, proliferative and promigration effects on mammalian fibroblasts. Scientific Reports, 8(17665), pp.1 – 10. doi: 10.1038/s41598-018-35816-3
Ulagesan, S., Sankaranarayanan, K. & Kuppusamy, A., 2018. Functional characterisation of bioactive peptide derived from terrestrial snail Cryptozona bistrialis and its wound-healing property in normal and diabetic-induced Wistar albino rats. Int Wound J, 15(3), pp.350 – 362. doi: 10.1111/iwj.12872
Ulagesan, S. & Kim, H.J., 2018. Antibacterial and Antifungal Activities of Proteins Extracted from Seven Different Snails. Applied Sciences, 8(8), pp.1 – 9. doi: 10.3390/app8081362
Vieira, T.C. R. G., Costa Filho, A. & Salgado, N.C., 2004. Acharan sulfate, the new glycosaminoglycan from Achatina fulica Bowdich 1822. Structural heterogeneity, metabolic labeling and localization in the body, mucus and the organic shell matrix. European Jurnal of Biochemistry, 271(4), pp.845 – 854. doi: 10.1111/j.1432-1033.2004.03989.x
Whittam A.J. et al., 2016. Challenges and Opportunities in Drug Delivery for Wound Healing. Adv Wound Care, 5(2), pp.79 – 88. doi: 10.1089%2Fwound.2014.0600
DOI: https://doi.org/10.22146/jtbb.90236
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