Penurunan Kadar IL-1β Makrofag Terpapar Agregat Bakteri Actinomycetemcomitans setelah Pemberian Minyak Atsiri Temu Putih

Juni Handajani(1*), Siti Fatimah(2), Ristini Asih(3), Antinah Latif(4)

(1) Bagian Biologi Mulut, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Program Studi Ilmu Keperawatan Gigi, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Program Studi Ilmu Keperawatan Gigi, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia
(4) Program Studi Ilmu Keperawatan Gigi, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author


IL-1β level of macrophage exposed to A. actinomycetemomitans decreases after administration Curcuma Zedoaria volatile oil. Activation of interleukin-1β (IL-1β) is a key regulator of the inflammatory response. Macrophage is a phagocytic mononuclear cell that plays an important role in innate and adaptive immune response. The cytokine secreted by macrophages in response to pathogen are IL-1, IL-6, IL-12, TNF-α and chemokine. Curcuma zedoaria volatile oil may have anti inflammation effect. The aim of this study was to investigate IL-1β level of macrophage exposed to A. actinomycetemcomitans after administration Curcuma zedoaria volatile oil. The subjects were 10 male Wistar rats, which divided into two groups (treatment and control), each group 5 rats. In the treatment group, 30,6 μl/ml Curcuma zedoaria volatile oil was administered per oral for 14 days and the control group used aquabidest. In the 7th days, 100 μl A. actinomycetemcomitans in CMC 2% were applied on the anterior gingival mandible for 7 days. Rats were anesthetized in the 15th days then macrophage was collected from peritoneal. Interleukin-1β level of macrophage was measured using ELISA kit (R&D Systems, USA). Data were analyzed using t-test. The result showed IL-1β level decreased after treatment. The comparison between treatment and control was significant difference (p<0.05). It can be concluded that Curcuma zedoaria volatile oil may have anti inflammation effect through reducing the IL-1β level of macrophage.


Curcuma zedoaria volatile oil; macrophage; IL-1β level; Curcuma zedoaria volatile oil; macrophage; IL-1β level

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Abbas AK, Lichtman AH, Phillai S. Cellular and molecular immunology. 7th ed. Philadelphia: Elsevier; 2013. H. 50-5.

Samaranayake L. Essential microbiology for dentistry. 4rd ed. Hongkong: Elsevier; 2012. H. 100-15.

Janeway CA Jr, Travers P, Walport M. Immunobiology: The immune system in health and disease. 7th ed. New York: Garland

Science; 2008. H. 2.19-2.20.

Abbas AK, Lichtman AH. Basic immunology: functions and disorders of the immune system. 3rd ed. Philadelphia: WB Saunders Company; 2010. H. 35-50.

Eales LJ. Immunology for life scientist. 2nd ed. England: Wiley; 2003. H. 90, 216-224.

Sunardi C, Dewi PNL, Sutedja L, Kardono LBS. Studi aktivitas antimikroba minyak atsiri dari rimpang Kaemperia rotunda L., Curcuma zedoaria Rosc. dan Curcuma mangga Val. Et ZIJP. Dalam: Prosiding Seminar Nasional Tumbuhan Obat Indonesia XXI. Surabaya: 27-28 Maret 2002.

Makanabe H, Maru N, Kuwabara A, Kamo T, Hirota M. Anti-inflammatory sesquiterpenes from Curcuma zedoaria,

n9474296. 2006> 18 Maret 2008.

Carvalho FR, Vassão RC, Nicoletti MA, Maria D, Carvalho FR. Effect of Curcuma zedoaria crude extract against tumor progression and immunomodulation. J Venom Anim Toxins incl Trop Dis. 2010; 16(2): 326-41.

Bugno A, Nicoletti MA, Almodóvar AAB, Pereira TC, Auricchio MT. Antimicrobial efficacy of Curcuma zedoaria extract as assessed by linear regression compared with commercial mouthrinses. Braz J Microbiol. 2007; 38(3): 427-32.

Kaushik ML, Jalalpure SS. Antiiflammatory efficacy of Curcuma zedoaria Rosc root extract. Asian J Pharm Clin Res. 2011; 4(3):


Handajani J. Temu putih (Curcuma zedoaria Rosc., Zingiberaceae) volatile oil increased phagocytic activity of neutrophils exposed to A. actinomycetemcomitans. Proceeding The International Symposium on Oral and Dental Sciences, 17-18 Januari 2013.

Naz S, Jabeen S, Iyas S, Manzoor F, Aslam F, Al A. Antibacterial activity of Curcuma Longa varieties against different strains of bacteria. Pak J Bot 2010; 42(1): 455-62.

Handajani J. Minyak atsiri temu putih (Curcuma zedoaria Rosc., Zingiberaceae) menurunkan ekspresi CD4+ pada gingiva terpapar A. actinomycetemcomitans. Maj Ked Gi, 2013; 20(1): 9-12.

Handajani J. Efek opsonisasi serum terhadap aktivitas fagositosis sel makrofag terpapar aggregat bakteri A. actinomycetemcomitans setelah pemberian minyak atsiri Temu Putih. Dentika Dent J. 2013; 17(3): 217-222.

Aberg CH, Sjödin B, Lakio L, Pussinen PJ, Johansson A, Claesson R. Presence of Aggregatibacter actinomycetemcomitans in young individuals: a 16- year clinical and microbiological follow-up study. J Clin Periodontol. 2009; 36(10): 815-22.

Bio Research. Protocol for performing a trypan blue viability test technical reference guide 24 November 2012.

Fujiwara N, Kobayashi K. Macrophages in inflammation. Curr Drug Targets Inflamm Allergy. 2005; 4(3): 281-6.

Ren K, Torres R. Role of interleukin-1β during pain and inflammation. Brain Res Rev. 2009; 60(1): 57-64.

Jang MK, Lee HJ, Kim JS, Ryu JH. A curcuminoid and two sesquiterpenoids from Curcuma zedoaria as inhibitors of nitric oxide

synthesis in activated macrophages. Arch Pharm Res. 2004; 27(12): 1220-5.

Tiemessen M, Kunzmann S, Schmidt-Weber C, Garssen J, Bruijnzeel-Koomen C, Knol E, van Hoffen E. Transforming growth factor-beta inhibits human antigen-specific CD4+ T cell

proliferation without modulating the cytokine response. Int Immunol. 2003; 15(12): 1495–504.

Seymour GJ, Taubman MA, Eastcott JW, Gemmell E, Smith DJ. CD29 expression on CD4+ gingival lymphocytes supports migration of activated memory T lymphocytes to diseased periodontal tissue. Oral Microbiol Immunol. 1997; 12(3): 129–34.


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