Effects of nano-hydroxyapatite paste on enamel microporosity after bleaching treatment


Nikmatus Sa'adah(1*), Gadis Meinar Sari(2), Elyana Asnar(3)

(1) Program Studi Magister Ilmu Kedokteran Dasar, Fakultas Kedokteran, Universitas Airlangga, Surabaya; Departemen Biologi Oral, Fakultas Kedokteran Gigi, Institut Ilmu Kesehatan Bhakti Wiyata, Kediri
(2) Departemen Ilmu Faal, Fakultas Kedokteran, Universitas Airlangga, Surabaya
(3) Departemen Ilmu Faal, Fakultas Kedokteran, Universitas Airlangga, Surabaya
(*) Corresponding Author


Bleaching or teeth whitening is a popular dental conservative treatment to enhance aesthetics. Bleaching treatment in many cases has successfully improved aesthetics but still remains the side effects. It can cause some changes in enamel structure leading to demineralization characterized by the emergence of microporosity on the enamel surface. Nano-hydroxyapatite is potential to re-mineralize earlyenamel lesions. This study aims to identify the effect of nano-hydroxyapatite on enamel microporosity after bleaching treatment. Twelve rabbits were randomly divided into two groups. The control and treatment group administered a placebo and nano-hydroxyapatite paste respectively, for 7 days after bleaching treatment. Once the treatment was completed, the rabbit was sacrificed, the jaw was decapitated, upper and lower incisors of the rabbit were separated from the jaw. Subsequently, the sample preparation wasconducted for examination of enamel microporosity depth by using scanning electron microscope. The results showed the control group had more microporositycompared to the treatment group. The independent t-test result showed that enamel microporosityhad a significant value of 0.003 (p <0.05) meaning that enamel microporosity between both groups had a significant difference. The conclusion of this study was administration of nan-hydroxyapatite paste reduced enamel microporosityin the administration of nano-hydroxyapatite pastes compared to the placebo pastes after bleaching treatment.


bleaching; tooth microporosity; nano-hydroxyapatite

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Souza GDMd, Santos LM, Fernandes CA, Dantas EDV, Galvão MR, Assunção IVd, Borges BCD. Sensitivity in dental bleaching and the use of anti-inflammatory agents. JSM Dent. 2014; 2(1): 1023.

2. Borges AB, Torres CR, Souza PA, Caneppele TM, Santos LF, Mag AC. Bleaching gels containing calcium and fluoride: effect on enamel erosion susceptibility. Int J Dent. 2012; 10: 1 – 6.

3. Ilyas M. Perbedaan kadar kalsium dalam saliva sebelum dan sesudah mengkonsumsi minuman ringan yang mengandung asam sitrat. JITEKGI. 2006; 3: 96 – 99.

4. Lussi A, Schaffner M, Jaeggi T. Dental erosion diagnosis and prevention in children and adults. Int Dent J. 2007; 57: 385 – 398.

5. Mount G, Hume W. Preservation and restoration of tooth structure. London: Mosby. 1998. 121-153.

6. Chandrasekar A, Sagadevan S, Dakshnamoorthy A. Synthesis and characterization of nano-hydroxyapatite (n-HAP) using the wet chemical technique. IJPS. 2013; 8(32): 1639 – 1645.

7. Low SB, Allen EP, Kontogiorgos ED. Reduction in dental hypersensitivity with nano-hydroxyapatite, potassium nitrate, sodium monofluorophosphate and antioxidants. Open Dent J. 2015; 9: 92 – 97.

8. Liwang B, Irmawati, Budipramana E. Kekerasan mikro enamel gigi permanen muda setelah aplikasi bahan pemutih gigi dan pasta remineralisasi. Dent. J. (Maj. Ked. Gigi). 2014; 47(4): 206 – 210.

9. Najibfard K, Chedjieu I, Ramalingam K, Amaechi B. Remineralization of early caries by a nano-hydroxyapatite dentifrice. J Clin Dent. 2011; 12(5): 1 – 5.

10. Huang S, Gao S, Yu H. Effect of nano-hydroxyapatite concentration on remineralization of initial enamel lesion in vitro. Biomed Mater. 2009; 4: 1 – 6.

11. Hedge A, Kumari S. Comparison of salivary calsium, phosphate, and alkaline phosphatase levels in children with early childhood caries after administration of milk, cheese, and GC tooth mousse: an in vivo study. J Clin Dent. 2014; 38(4): 318 – 325.

12. Wiryani M, Sujatmiko B, Bikarindrasari R. Pengaruh lama aplikasi bahan remineralisasi casein phosphopeptide-amorphous calcium phosphate fluoride (CPP-ACPF) terhadap kekerasan email. Majalah Kedokteran Gigi Indonesia. 2016; 2(3): 141 – 146.

13. Pepla E, Besherat LK, Palala G, Tenore G, Migilau G. Nano-hydroxyapatite and its applications in preventive, restorative and regenerative dentistry: a review of literature. Ann Stomatol. 2014; 5(3): 108 – 114.

DOI: https://doi.org/10.22146/majkedgiind.24888

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