Effect of nano silica coating concentration on reducing acrylic resin denture cytotoxicity

https://doi.org/10.22146/majkedgiind.37166

Gene Rizky Natalia Gunawan(1*), Titik Ismiyati(2), Haryo Mustiko Dipoyono(3)

(1) RSGMP UNSOED Purwokerto, Central Java Specialty Study Program of Prosthodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(2) Department of Prosthodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(3) Department of Prosthodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author

Abstract


Acrylic resin is a denture base material mostly used in the fabrication of removable partial denture and complete denture because it is easy to process, economical and aesthetically satisfying. However, the acrylic resin has a weakness i.e. residual monomer content which causes cytotoxic effect to human cells. Nano-silica coating could be used on the acrylic resin to reduce residual monomer content. The purpose of this study was to determine the effect of nano-silica coating concentration on the cytotoxicity of acrylic resin denture base material. Twenty-four acrylic resin disc-shaped specimens (5 mm in diameter and 2 mm in thickness) were divided into 4 groups (n=6), i.e. control, group with application of 0.5%,
1%, and 2% nano silica coating. The specimens on the silica coating groups were silica coated using dip-coating method. After the coating was completed, the specimens were added into cell fibroblast culture (vero cell line). Cytotoxicity tests
were done by MTT assay. The optical density was measured using ELISA plate reader to determine the percentage of cell viability. The highest mean of cell viability was shown in 0.5% concentration (96.78 ± 1.71) and the lowest mean was shown in the control group (80.32 ± 6.53). One-way ANOVA showed that there were significant differences among these groups. LSD tests showed that there was significant difference among all of the groups. This study concluded that nano silica coating concentration has a significant effect on the cytotoxicity of acrylic resin denture base materials.

Keywords


acrylic resin; cytotoxicity; fibroblast cell; nano silica coating

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References

1. Craig RG. Restorative dental materials 13th Ed. St. Louis: CV Mosby; 2011.

2. Anusavice KJ, Phillips RW, Shen C, Rawls HR. Phillips‘ science of dental materials, 12th ed. St. Louis: Elsevier; 2013. 165-166, 721-722.

3. Carr AB, Brown DT. McCracken’s removable partial prosthodontics 12th Ed. Singapura: Mosby Elsevier; 2012. 7, 106-107, 346.

4. Powers JM, Sakaguchi RL. Craig‘s restorative dental materials 12th ed. St.Louis: Elsevier; 2012. 524-544.Combe EC and Grant AA. Notes of dental material, 6th Ed. New York: Churchill Livingstone.; 1992. 26-28, 79-120, 157-161, 224, 262-269.

5. Alnamel HA, Mudhaffer M. The Effect of silicon dioxide nano fillers reinforcement on some properties of heat cured polymethylmetacryate denture base material. J Bagh College Dentistry. 2014; 26(1): 32-36.

6. Sodagar A, Bahador A, Khalil S, Shahroudi AS, Kassaee MZ. The Effect of TiO2 dan SiO2 nanoparticles on some flexural strength of polymethyl metacrylate acrlic resin. J Prosthodont Res. 2013; 57(1): 15-19. doi: 10.1016/j.jpor.2012.05.001

7. Azuma A, Akiba N, Minakuchi S. Hydrophilic surface modification of acrylic denture base material by silica coating and its influence on Candida albicans adherence. J Med Dent Sci. 2012; 59(1): 1-7.

8. Kamonwanon P, Yodmongkol S, Chantarachindawong R, Thaweboon, S, Thaweboon B, Srikhirin T. Wear resistance of a modified polymethyl methacrylate artificial tooth compared to five commercially available artificial tooth materials. J Prosthet Dent. 2015; 114(2): 286-292. doi: 10.1016/j.prosdent.2015.01.013

9. Yoshizaki T, Akiba N, Inokoshi M, Shimada M, Minakuchi S. Hydrophilic nano-silica coating agents with platinum and diamond nanoparticles for denture base materials. Dent Mater J. 2017; 36(3): 333-339. doi: 10.4012/dmj.2016-243

10. Zayed SM, Alshimy AM, Fahmy AE. Effect of surface treated silicon dioxide nanoparticles on some mechanical properties of maxillofacial silicone elastomer. Int J Biomater. 2014; 2014: 750398. doi: 0.1155/2014/750398

11. Kim IY, Joachim E, Choi H, Kim K. Toxicity of silica nanoparticles depends on size, dose and cell type. Nanomedicine. 2015; 11(6): 1407-1416. doi: 10.1016/j.nano.2015.03.004

12. Jung EK, Kim H, An SS, Maeng EH, Kim MK, Song YJ. In vitro cytotoxicity of SiO2 or ZNO nanoparticles with different sizes and surface charges on human glioblastoma cells. Int J Nanomedicine. 2014; 9(2): 235-241. doi: 10.2147/IJN.S57936

13. Kumar R, Roy I, Ohulchanskky TY, Vathy LA, Bergey EJ, Sajjad M. In vivo biodistribution and clearance studies using multimodal organically modified silica nanoparticles. ACS Nano. 2010; 4(2): 699-708. doi: 10.1021/nn901146y

14. Nishimori H, Kondoh M, Isoda K, Tsunoda S, Tsutsumi Y, Yagi K. Silica nanoparticles as hepatoxicants. Eur J Pharm Biopharm. 2009; 72(3): 496-501. doi: 10.1016/j.ejpb.2009.02.005

15. ISO 10993-5. Biological evaluation of medical devices-part 5: tests for in vitro cytotoxicity, International Organization for standarization. Geneva; 2009. 30-34.

16. Amano D, Ueda T, Sugiyama T. Improved brushing durability of titanium dioxide coating on polymethylmethacrylate substrate by prior treatment with acryloxypropyl trimethoxysilane¬based agent for denture application. Dent Mater J. 2010; 29(1): 97–103. doi: 10.4012/dmj.2009-073

17. Ammerman NC, Beier Sexton M, Azad AF. Growth and maintenanace of vero cell lines. Curr.Protoc.Microbiol. 2008. 1-7. doi: 10.1002/9780471729259.mca04es11

18. Ahuja S, Babu J, Wicks R, Garcia-Godoy F, Tipton D. Cytotoxic effects of three deture base materials on gingival epithelial cells and fibroblast: an in vitro study. Int J of Experimental Dental Science. 2015; 4(1): 11-16. doi: 10.5005/jp-journals-10029-1088

19. Celebi N, Yuzuglu B, Canay S, Yucel U. Effect of polymerization methods on the residual monomer level of acrylic resin denture base polymers. Polym Adv Technol. 2008; 19(3): 201–206. doi: 10.1002/pat.996

20. Suhono RS. Pengaruh silica coating pada plat gigi tiruan resin akrilik haet cured tehadap kekerasan permukaan dan monomer sisa. UGM Yogyakarta: Thesisl; 2018. 58.

21. Wang Y, Xiaojun W, Wei Y, Yuanming Z, Banghu X, Mingbo Y. Aggregate of nanoparticles: rheological and mechanical properties. Nanoscale Res Lett. 2011; 6(1): 114. doi: 10.1186/1556-276X-6-114

22. Tadano T, Rui Z, Yoshio M, Toru H, Shoichiro Y. A new mechanism for the silica nanoparticle dispersion–agglomeration transition in a poly(methyl methacrylate)/silica hybrid suspension. Polymer Journal. 2014; 46: 342–348. doi: 10.1038/pj.2014.6

23. Tahmasebpoor M, Ghasemi SA, Rahimvandi N, Badamchizadeh P. Model based on electrostatic repulsion and hydrogen bond forces to estimate the size of nanoparticles agglomerate in fluidization. Ind. Eng. Chem. Res. 2016; 55: 12939-12940.

24. Wu X, Sacher E, Meunier M. The effects of hydrogen bonds on the adhesion of inorganic oxide particles on hydrophilic silicon surfaces. J. Appl. Phys. 2002; 86 (3): 1744-1748.



DOI: https://doi.org/10.22146/majkedgiind.37166

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