Effect of freeze-drying process of collagen-activated platelet-rich plasma on transforming growth factor-β1 level


Kwartarini Murdiastuti(1*), Fitri Yuniawati(2), Dahlia Herawati(3), Nunuk Purwanti(4), Dyah Ayu Mira Oktarina(5)

(1) Department of Periodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(2) Master of Clinical Dental Sciences, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(3) Department of Periodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(4) Department Dental Biomedical Sciences, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(5) Department of Dermatology and Venereology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author


Periodontal tissue damage requires regenerative material to repair the damage. Platelet-rich plasma (PRP) is known as a regenerative material from blood which contains high level of growth factor that plays a role in wound healing and tissue remodeling. However PRP has a weakness, i.e. it is too watery so it is easily dissolved in the oral cavity, and should be used immediately after preparation. Therefore PRP storage method is needed to increase the benefits of PRP. The addition of collagen to PRP serves as a scaffold as well as an activator that stimulates the release of growth factors. One method of storing PRP is by freeze-drying process. The purpose of this study was to analyze the effect of freeze-drying process of collagen-activated PRP (PRP+C) on transforming growth factor-β1 (TGF-β1) levels. Transforming growth factor-β1 is a cytokine content in PRP, that plays a role in bone remodeling and is an important stimulator for osteoblast formation, causing chemotaxis, osteoblast proliferation and differentiation. In this study, PRP was produced from peripheral blood probandus. Platelet-rich plasma was then activated with collagen (PRP+C), and divided into two groups: freeze-dried PRP collagen (FD PRP+C); and non freeze-dried PRP+collagen (PRP+C). Transforming growth factor-β1 levels were measured using the ELISA method, followed by independent t-test. The TGF-B1 level of FD PRP+C group was significantly higher than PRP+C group (p<0.05). From this study it can be concluded that freeze-dried collagen-activated PRP has an effect to increase TGF-β1 level.


collagen; freeze-drying; platelet-rich plasma; TGF-β1

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1. Heber A, Luis G, Sandra R, Edward I, César L, Hernán V. Clinical effect of platelet rich plasma
in the treatment of periodontal intrabony defects. Systematic review and meta-analysis. J Oral Res. 2017; 6(4): 97-104. doi: 10.17126/joralres.2017.028

2. Graziani F, Ivanovski S, Cei S, Ducci F, Tonetti M, Gabriele M. The in vivo effect of different PRP
concentrations on osteoblasts and fibroblast. Clin Oral Implants Res. 2006; 17(2): 212-219.
doi: 10.1111/j.1600-0501.2005.01203.x

3. Nakatani Y, Agata H, Sumita Y, Koga T, Asahita I. Efficacy of freeze-dried platelet-rich
plasma in bone enineering. Arch Oral Biol. 2016; 73(2017): 172-178. doi: 10.1016/j.archoralbio.2016.10.006

4. Ince B, Yildirim MEC, Dadaci M, Avunduk MC, Savaci N. Comparison of the efficacy of homologous and autologous plateletrich plasma (PRP) for treating androgenic alopecia. Aesthetic Plast Surg. 2017; 42(1): 297-303. doi: 10.1007/s00266-017-1004-y

5. Prabhu R, Vijayakumar C, Bosco CA, Balagurunathan K, Kalaiarasi R, Raja ES, Swetha T. Efficacy of homologous, plateletrich plasma dressing in chronic non-healing ulcers: an observational study. Cureus. 2018; 10(2): 1-10. doi: 10.7759/cureus.2145

6. Pan L, Yang Z, Yuk KS, Hoon KY, Yuedong S, Xu J. Growth factor release from lyophilized porcine platelet- rich plasma: quantitative analysis and implications for clinical applications. J Aesthetic Plast Surg. 2015; 40(1): 157–163. doi: 10.1007/s00266-015-0580-y

7. Shiga Y, Kubota G, Orita S, Inage K, Kamoda H, Yamashita M, Iseki T, Ito M, Yamauchi
K, Eguchi Y, Sainoh T, Sato J, Fujimoto K, Abe K, Kanamoto H, Inoue M, Kinoshita H, Furuya T, Koda M, Aoki Y, Tonoye T, Takashi K, Ohtori S. Freeze-Dried human platelet-rich
plasma retain activation and growth factors expression after an eight- week preservation
period. Asian Spine J. 2017; 11(3): 329-336. doi: 10.4184/asj.2017.11.3.329

8. Textor J. Platelet-Rich Plasma (PRP) as a therapeutic agent: platelet biology, growth factors and a review of the literature. Platelet-Rich Plasma. 2014; 61-94. doi: 10.1007/978-3-642-40117-6_2

9. Li Z, Delaney MK, O’Brien KA, Du X. Signaling during platelet adhesion and activation.
Artioscler Thromb Vasc Biol. 2010; 30(12): 2341-2349. doi: 10.1161/ATVBAHA.110.207522

10. Fufa D, Shealy B, Jacobson M, Kevy S, Murray MM. Activation of platelet-rich plasma using soluble type I collagen. J Oral Maxillofac Surg. 2008; 66: 684-690. doi: 10.1016/j.joms.2007.06.635

11. Islam MS, Aryasomayajula A, Selvaganapathy PR. A Review on macroscale and microscale
cell lysis method. Micromachines. 2017; 8(3): 83. doi: 10.3390/mi8030083

12. Usri, Kosterman. Penerapan teknologi liofilisasi dan radiasi sinar ɣ pada pembuatan
graf di Indonesia. Jurnal Material Kedokteran Gigi. 2012; 1(2): 153-157

DOI: https://doi.org/10.22146/majkedgiind.40197

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