A potential of Jasminum sambac (L.) Aiton leaf nano-extract as spray treatment of gingivitis-induced Sprague Dawley rats


Archadian Nuryanti(1*), Puspa Ayu Aprilia Larasati(2), Alda Risma Almeyda(3), Monica Agnesia Marandof(4), Catherine Marcia Irawan(5)

(1) Departement of Dental Biomedical Sciences, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(2) Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(3) Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(4) Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(5) Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author


Gingivitis is the second most common disease that occurs in the oral cavity. The use of chlorhexidine as a gingivitis therapy agent has some side effects such as allergic reactions and bacterial resistance. Jasminum Sambac (L.) Aiton leaf nano-extract contains flavonoids, saponins, and tannins compounds reported to have an influence on wound healing process. The aim of this research was to determine the effect of observation time and concentrations of Jasminum sambac (L.) Aiton leaf nano-extract on the number of neutrophils, macrophages, and angiogenesis in the treatment of gingivitis-induced Sprague Dawley rats. Sixty male Sprague Dawley rats aged 2.5-3 months with body weight of 200-250 g were anesthetized with ketamine HCl (10 mg/kg BW) and xylazine (2 mg/kg BW) then induced using silk ligature 3.0 on the interdental of the mandibular incisor. The rats were divided into five groups and got daily spray using distilled water (negative control), nano-extract at a concentration of 40%, 45%, 50%, and 0.12% chlorhexidine (positive control). The rats were euthanized on the 1st, 3rd, 5th, and 7th days post-treatment. The tissues were processed histologically with HE staining. The number of neutrophils, macrophages, and angiogenesis were counted using a microscope (400x) and OptiLab Viewer® (13x) at five fields of view. The result of the Two Way ANOVA test showed that there were significant effect of concentrations and observation time, and interaction of both observation time and concentrations on the number of neutrophils, macrophages, and angiogenesis (p < 0.05). It can be concluded that observation time and concentrations of Jasminum sambac (L.) Aiton leaf nano-extract affected the number of neutrophils, macrophage, and angiogenesis in the treatment of gingivitis-induced Sprague Dawley rat (p < 0.05).


angiogenesis; gingivitis; Jasminum sambac (L.) Aiton; macrophage; nano-extract; neutrophil

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1. Aboodi GM, Sima C, Moffa EB, Crosara KTB, Xiao Y, Siqueira WL, Glogauer M. Salivary
cytoprotective protein inflammation and resolution during experimental gingivitis – a
pilot study. Front Cell Infect Microbiol. 2016; 5(92):1-12. doi: 10.3389/fcimb.2015.00092

2. Debnath K, Chatterjee A, Rao NKH. A Comparative evaluation of the efficacy of
curcumin and chlorhexidine mouthrinses on clinical inflammatory parameters of gingivitis:
a double-blinded randomized controlled clinical study. JISP. 2017: 132-137.
doi: 10.4103/jisp.jisp_136_17

3. Notohartojo IT, Suratri MAL. Tooth brushing, consumption of fruits and vegetables, physical
activity, diabetes mellitus with periodontal teeth in Indonesia, 2013. Health System
Research Bulletin. 2016; 19(4): 219-225. doi: 10.22435/hsr.v19i4.6839.219-225.

4. Wijayanto R, Herawati D, Sudibyo. Differences in the effectiveness of topical hyaluronic acid
gel and metronidazole gel on periodontal tissue healing after curettage in chronic periodontitis.
J Ked Gigi. 2014; 5(3): 307-325.

5. Martien R, Adhyatmika I, Farida V, Sari D. Development of nanoparticle technology
as a drug delivery system. Pharmaceutical Magazine. 2012; 8(1): 134.

6. Nurdiana, Ulya I, Putra I. Effect of administering jasmine leaf extract gel (Jasminum sambac L. Ait) on the number of skin fibroblasts in healing second degree burns in wistar strain white
rats (Rattus norvegicus). Journal of Nursing Science. 2016; 4(1): 1-7.
doi: 10.21776/ub.jik.2016.004.01.1.

7. Pence B, Woods J. Exercise, obesity, and cutaneous wound healing: evidence from
rodent and human studies. Adv Wound Care. 2014; 3(1): 72. doi: 10.1089/wound.2012.0377.

8. Chen Y, Lu Y, Lee RJ, Xiang G. Nano encapsulated curcumin: and its potential for
biomedical applications. Int J Nanomedicine. 2020; 15: 3099-3120.
doi: 10.2147/IJN.S210320

9. Souza N, Rodrigues A, Cardoso C, Pardo H, Faccio R, Mombru A, Galzerani J, Lima
O, Sergeenko S, Araujo-Moreira F. Physical properties of nanofluid suspension of
ferromagnetic graphite with high zeta potential. Phys Lett A. 2011; 376(2012): 544-546.
doi: 10.1016/j.physleta.2011.11.050.

10. Omer-Cihangir R, Baser U, Kucukgergin C, Aykol-Sahin G, Huck O, Yalcin F. Impact of
Smoking on Neutrophil Enzyme Levels in Gingivitis: A Case-Control Study. Int J Environ
Res Public Health. 2021; 18(15): 1-9. doi: 10.3390/ijerph18158075.

11. Neck JV, Tuk B, Barritault D, Tong M. Heparan sulfate proteoglycan mimetics promote
tissue regeneration: an overview. Tissue Regeneration. 2012; 69-92.

12. Tamara A, Oktiani BW, Taufiqurrahman I. Effect of propolis kelulut (G.thoracica)
flavonoid extract on the number of neutrophil cells in periodontitis in vivo study in male
Wistar Rats (Rattus norvegicus). Dentine. 2019; 3(1): 10-16.

13. Sengar N, Joshi A, Prasad SK, Hemalatha S. Anti-inflamatort, analgesic and anti-pyretic
activities of standardized root extract of Jasminum sambac. J Ethnopharmacol. 2015;
160: 140-8. doi: 10.1016/j.jep.2014.11.039.

14. Wibawani L, Wahyuni ES, Utami YW. The Effect of topical administration of ethanol
extract of jasmine (Jasminum sambac L. Ait) leaves on increased contraction of second degree burns in White Rats (Rattus norvegicus) Wistar Strain. FKUB Health
Magazine. 2015; 2(4):196-206.

15. Bano I, Arshad M, Yasin T, Ghauri M, Younus M. Chitosan: A Potential biopolymer for wound
management. Int J Biol Macromol. 2017; 102: 380-383. doi: 10.1016/j.ijbiomac.2017.04.047

16. Dewantari KT, Yuliani S, Yasni. Extraction and characterization of red betel (Piper crocatum)
Extract Nanoparticles. J Postharvest. 2013; 10(2): 58-65. doi: 10.21082/jpasca.v10n2.2013.58-65

17. Gowdhami T, Rajalakhsmi A, Sugumar N, Valliappan R. Evaluation of antimicrobial
activity of different solvent extract of aromatic plant: Jasminum Sambac Linn. JCPRC.
2015; 7(11): 139.

18. Filardi ES, Kröger AP, Blanco FJ, Nieto C, Bragado R, Palomero MI, Bernabéu C, Vega
MA, and Corbí AL. Activin skews macrophage polarization by promoting a proinflammatory
phenotype and inhibiting the acquisition of anti-inflammatory macrophage markers.
Immunobiology. 2011; 117(19): 5092–5101. doi: 10.1182/blood-2010-09-306993

19. Kmiec M, Pighinelli L, Tedesco M, Silva M, Reis V. Chitosan-Properties and applications
in dentistry. Adv Tissue Eng Med. 2017; 2(4): 00035. doi: 10.15406/atroa.2017.02.00035.

20. Ma Q, Jiang J, Yuan X, Qiu K, Zhu W. Comparative antitumor and antiinflammatory
effects of flavonoids, saponins, polysaccharides, essential oil, coumarin and
alkaloids from Cirsium Japonicum DC. Food Chem Toxicol. 2019; 125(2019): 422-429. doi: 10.1016/j.fct.2019.01.020.

21. Brancato S, Albina J. Wound macrophages as key regulators of repair. Am J Pathol. 2011;
178(1): 19-25. doi: 10.1016/j.ajpath.2010.08.003

22. Koh T, Pietro L. Inflammation and wound healing: the role of the macrophage. Expert
Rev Mol Med. 2011; 13(23): 1-12. doi: 10/1017/S1462399411001943.

23. Kong J, Gao X. Macrophage polarization: a key event in the secondary phase of acute spinal
cord injury. J Cell Mol Med. 2016; 21(5): 941-954. doi: 10.1111/jcmm.13034.

24. Li K, Diao Y, Zhang H, Wang S, Zhang Z, Yu B, Huang S, Yang H. Tannin extracts from
immature fruits of Terminalia chebula Fructus Retz. promote cutaneous wound healing in
rats. BMC Complementary and Alternative Medicine. 2011; 11(86): 1-9.
doi: 10.1186/1472-6882-11-86.

25. Larjava H. Oral Wound Healing. Oxford: Willey-Blackwell; 2012. 175-176.

26. Nofikasari I, Rufaida A, Aqmarina CD, Failasofia, Fauzia AR, Handajani J. Effect of
topical application of pandan wangi extract gel on gingival wound healing. Majalah Kedokteran
Gigi Indonesia. 2016; 2(2): 53-59. doi: 10.22146/majkedgiind.9896.

27. Nugroho A, Elfiah U, Nomasari R. Effect of extract gel and cucumber powder (Cucumis
sativus) on angiogenesis in healing second degree burns in Wistar Rats. Health Library.
2016; 4(3): 443-448.

28. Budi HS, Soesilowati P, Imanina Z. Histopathological features of healing tooth
extraction wounds in macrophages and neovascular with administration of ambon
banana stem sap. Majalah Kedokteran Gigi Indonesia. 2017; 3(3): 121-127.
doi: 10.22146/majkedgiind.17454

DOI: https://doi.org/10.22146/majkedgiind.69468

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