Anti-inflammatory loading of cinnamaldehyde on artificial bone scaffolds against the process of bone regeneration

https://doi.org/10.22146/majkedgiind.87347

Anne Handrini Dewi(1), Andi Triawan(2*), John Jansen(3)

(1) Department of Biomedical Medicine, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(2) Department of Orthodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(3) Department of Biomaterials, Radboud University Medical Center, 6500 HB Nijmegen
(*) Corresponding Author

Abstract


Cinnamaldehyde is an organic component in cinnamon and has anti-inflammatory, antibacterial, and osteogenic properties. Loading cinnamaldehyde with a concentration of 4% into an artificial scaffold from gypsum calcium carbonate hydrogel could reduce inflammation, accelerate healing, and promote new bone regeneration at bone implantation surgery. The aim of this study was to investigate the impact of cinnamaldehyde loaded on gypsum-hydrogel calcium carbonate on the process of wound healing and bone regeneration in rats. Implantation of bone scaffolding from gypsum hydrogel calcium carbonate with an addition of cinnamaldehyde was performed on 20 5-month-old Sprague-Dawley rats weighing 300-350 g in an artificial defect of condyle femoris bone dexter and sinister. Group A was a control with gypsum only, while groups B, C, and D were given gypsum and CaCO3 with a combination of cinnamaldehyde or dehydrothermal treatment (DHT). Euthanasia was performed after implantation at 1, 4 and 8 weeks for 4 groups (n = 3). Femoris condyle bone was cut, made into histological preparations by hematoxylin eosin (HE) staining, and analyzed descriptively. The results showed that the loading of cinnamaldehyde on the scaffold was effective in weeks 1 and 4, but after 8 weeks of implantation, cinnamaldehyde was observed to inhibit defect closure. The cinnamaldehyde group combined with dehydrothermal (DHT) treatment was found to be better than those without DHT.

Keywords


bone scaffold; cinnamaldehyde; new bone regeneration



References

1. Lee Y, Huang J, Bing Z, Yuan K, Yang J, Cai M, Zhou S, Yang B, Teng W, Li W, Wang
Y. PH-responsive cinnamaldehyde-TiO 2 nanotube coating: fabrication and functions in
a simulated diabetes condition. J Mater Sci: Mater Med. 2022; 33(63): 1-14.
doi: 10.1007/s10856-022-06683-2

2. Vasconcelos NG, Croda J, Simionatto S. Antibacterial mechanisms of cinnamon and its
constituents: A review. Microb Pathog. 2018; 120: 198-203.
doi: 10.1016/j.micpath.2018.04.036

3. Yanakiev S. Effects of Cinnamon (Cinnamomum spp.) in dentistry: a review.
Molecules. 2020; 25(18): 4184. doi:10.3390/molecules 25184184

4. Dewi AH, Ana ID, Jansen J. Calcium carbonate hidrogel construct with cynnamaldehyde
incorporated to control inflammation during surgical procedure. J Biomed Mater Res.
2016; 104(3): 768–774. doi: 10.1002/jbm.a.35571

5. Cheng WX, Zhong S, Meng, Zheng NY, Zhang P, Wang Y, et al. Cinnamaldehyde inhibits
inflammation of human synoviocyte cells through regulation of Jak/Stat pathway and
ameliorates collagen-induced arthritis in rats. J Pharm Exp Ther. 2020; 373(2): 302–310.
doi: 10.1124/jpet.119.262907

6. Kim NY, Trinh NT, Ahn SG, Kim SA. Cinnamaldehyde protects against oxidative stress and inhibits the TNF-α-induced inflammatory response in human umbilical vein endothelial cells. Int J Mol Med. 2020; 46(1): 449–457. doi: 10.3892/ijmm.2020.4582.

7. Koppikar SJ, Choudhari S, Suryavanshi SA, Kumari S, Chattopadhyay S, Kaul-Ghanekar R. Aqueous cinnamon rxtract (ACE-c) from the bark of Cinnamomum cassia causes apoptosis in human cervical cancer cell line (SiHa) through loss of mitochondrial membrane potential. BMC Canc. 2010; 10(1): 210. doi: 10.1186/1471-2407-10-210

8. Mollazadeh H, Hosseinzadeh H. Cinnamon effects on metabolic syndrome: a review
based on its mechanisms. Iran J Basic Med Sci. 2016; 19(12): 1258–1270.
doi: 10.22038/ijbms.2016.7906

9. Trinh NT, Dumas E, Thanh ML, Degraeve P, Ben Amara C, Gharsallaoui A, Oulahal N. Effect of a Vietnamese Cinnamomum cassia essential oil and its major component trans-cinnamaldehyde on the cell viability, membrane integrity, membrane fluidity, and proton motive force of Listeria innocua. Can J Microbiol. 2015; 61(4): 263–271. doi: 10.1139/cjm-2014-0481

10. Yap PS, Krishnan T, Chan KG, Lim SH. Antibacterial mode of action of Cinnamomum
verum bark essential oil, alone and in combination with piperacillin, against a multidrug-
resistant Escherichia coli strain. J Microbiol Biotechnol. 2015; 25(8): 1299–1306.
doi: 10.4014/jmb.1407.07054

11. Dewi AH, Yulianto DK, Rochmadi, Siswomihardjo W, Ana ID. Effect of cinnamaldehyde, an anti-inflammatory agent, on the surface characteristics of a plaster of Paris – CaCO3 hidrogel for bone substitution in Biomedicine. International Journal of Technology. 2020; 11(5): 963-973.
doi: 10.14716/ijtech.v11i5.4313

12. Dewi AH, Yulianto DK, Siswomihardjo W, Rochmadi R, Ana ID. Effect of dehydrothermal
treatment on the mechanical properties and biocompatibility of plaster of Paris–CaCO3
hydrogel loaded with cinnamaldehyde for biomedical purposes. Nat Prod Commun. 2021; 16(1): 1–13. doi: 10.1177/1934578X20984609

13. Sunarso S, Sutarno S, Tsuru K, Ana ID, Ishikawa K. Effect of crosslinking to the mechanical property of apatite gelatin hybrid for bone substitution purposes. Indonesian Journal of Chemistry. 2011; 11(3): 267-272. doi: 10.22146/ijc.21391

14. Sarikaya B, Aydin HM. Collagen/betatricalcium phosphate based synthetic bone grafts via dehydrothermal processing. Biomed Res Int. 2015; 2015: 1-9. doi: 10.1155/2015/576532

15. Weng SJ, Yan DY, Tang, Shen ZJ, Wu ZY, Xie ZJ, et al. Combined treatment with Cinnamaldehyde and beta-TCP had an additive effect on bone formation and angiogenesis in
critical size calvarial defect in ovariectomized rats. Biomed Pharmacother. 2019; 109: 573–
81. doi: 10.1016/j.biopha.2018.10.085

16. Wu Z, Yan D, Xie, Weng S, Zhou Q, Li H, et al. Combined treatment with cinnamaldehyde
and PTH enhances the therapeutic effect on glucocorticoid-induced osteoporosis through
inhibiting osteoclastogenesis and promoting osteoblastogenesis. Biochem Biophys Res
Commun. 2018; 505(3): 945–950. doi: 10.1016/j.bbrc.2018.10.039

17. Wu Z, Weng S, Yan, Xie Z, Zhou Q, Li H, et al. Administration of cinnamaldehyde
promotes osteogenesis in ovariectomized rats and differentiation of osteoblast in vitro. J
Pharmacol Sci. 2018; 138(1): 63–70. doi: 10.1016/j.jphs.2018.09.002

18. Dewi AH, Ana ID, Wolke J, Jansen J. Behavior of plaster of Paris-calcium carbonate composite as bone substitute. A study in rats. J Biomed Mater Res A. 2013: 101(8): 2143–2150.
doi: 10.1002/jbm.a.34513

19. Dewi AH, Ana ID, Jansen J. Preparation of a calcium carbonate-based bone substitute
with cinnamaldehyde crosslinking agent with potential anti-inflammatory properties. J
Biomed Mater Res A. 2017; 105(4): 1055–1062. doi: 10.1002/jbm.a.35990

20. Babu NV, Kannan S. Enhanced delivery of baicalein using cinnamaldehyde cross-linked
chitosan nanoparticle inducing apoptosis. Int J Biol Macromol. 2012; 51(5): 1103-1108.
doi: 10.1016/j.ijbiomac. 2012.08.038



DOI: https://doi.org/10.22146/majkedgiind.87347

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