* Corresponding Author
Pengaruh Aplikasi Gel Ekstrak Kulit Citrus Sinensis terhadap Epitelisasi pada Penyembuhan Luka Gingiva Tikus Sprague Dawley
https://doi.org/10.22146/majkedgiind.9012 Aqilla Tiara Kartiningtyas(1*), Prayitno Prayitno(2), Sri Pramestri Lastianny(3)
(1) Program Studi Pendidikan Dokter Gigi, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Bagian Periodonsia, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Bagian Periodonsia, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author
Abstract
Penyembuhan luka merupakan mekanisme pertahanan jaringan, dengan epitelisasi sebagai salah satu parameternya. Kulit jeruk manis memiliki potensi dalam penyembuhan luka karena mengandung nutrisi yang memiliki peran dalam mempercepat penyembuhan luka. Penelitian ini bertujuan untuk mengetahui efek aplikasi topikal gel ekstrak kulit jeruk
manis terhadap epitelisasi pada proses penyembuhan luka gingiva labial tikus Sprague Dawley. Dua puluh tujuh ekor tikus Sprague Dawley, berumur 2-3 bulan, dibagi dalam 3 kelompok, kontrol positif, perlakuan, dan kontrol negatif. Perlukaan pada gingiva labial mandibula dibuat dengan punch biopsy berdiameter 2,5 mm. Luka pada kelompok kontrol
positif diaplikasikan Aloclair, kelompok perlakuan diaplikasikan gel ekstrak kulit jeruk manis 10%, dan kelompok kontrol negatif diaplikasikan CMC-Na, masing-masing 2 kali sehari selama 1 menit secara topikal. Tiga ekor tikus dari tiap kelompok didekapitasi masing-masing pada hari ke-3, 7, dan 14. Jaringan luka diambil dan dibuat sediaan histologis dengan pengecatan Hemaktosilin Eosin. Pengukuran ketebalan epitel dilakukan dengan menggunakan Optilab yang dipasang pada mikroskop cahaya. Data ketebalan epitel dianalisis dengan menggunakan ANAVA dua jalur. Hasil pengukuran ketebalan epitel menunjukkan perbedaan yang tidak signifikan pada kelompok kontrol positif dengan perlakuan (p>0,05), namun menunjukkan perbedaan yang signifikan pada kelompok perlakuan dengan kontrol negatif (p<0,05) pada hari 3, 7, dan 14 setelah perlukaan. Kesimpulan dari penelitian ini adalah aplikasi topikal gel ekstrak kulit jeruk manis mempercepat epitelisasi pada proses penyembuhan luka gingiva labial tikus Sprague Dawley.
Effect of Topical Application of Sweet Orange (Citrus sinensis) Peel Extract Gel on Epithelialization of Labial Gingival Wound Healing: In Vivo Studies in Sprague Dawley Rats. Wound healing is a defense mechanism from complex biological phenomenon, in which epithelialization occurs as one of its parameters. Sweet orange peel
contains nutrients that have role in enhancing wound healing process. The objective of this research is to determine the effect of topical application of sweet orange peel extract gel on epithelialization of wound healing process in labial mandibular gingiva of Sprague Dawley rats. Twenty seven Sprague Dawley rats were divided into 3 groups: positive
control, treatment, and negative control. Labial mandibular gingival was wounded using 2.5 mm diameter punch biopsy. Topically, each wound of positive control group was administered Aloclair, treatment group was administered 10% sweet orange peel extract gel, and negative control group was administered CMC-Na, twice a day for 1 minute. Three rats from each group were sacrificed for histological evaluation at 3, 7, and 14 days and the specimens were stained with HE. The measurement of the epithelial thickness used Optilab installed on microscope. The data obtained from the measurement was analyzed using two-way ANOVA. There was insignificant difference between positive control group and treatment group (p>0.05) while there was significant difference between treatment group and negative control group (p<0.05) observed at 3, 7, and 14 days. The conclusion of this research is that the application of sweet orange peel extract gel accelerates epithelialization of wound healing process in labial mandibular gingiva of Sprague Dawley rats.
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Bhat SB. SRB’s Surgery for Dental Students, 2nd edition. Jaypee. New Delhi. 2014; 1.
Freedman M, Stassen LFA. Commonly used topical oral wound dressing materials in dental and surgical practice: a literature review. J Ir Dent Assoc. 2013; 59(4); 190.
Fedi PF, Vernino AR, Hughes E. The periodontic syllabus, 5th edition. lippincott williams and Wilkins. Philadelphia. 2008; 132-
Andreasen JO, Andreasen FM, Andersson L. Textbook and color atlas of traumatic injuries to the teeth. 4th edition. Wiley-Blackwell.
Oxford. 2013; 1-10, 36-37.
Lynch ME, Craig KD, Peng PWH. Clinical pain management: a practical guide, wileyblackwell. Oxford. 2011.
Patel VF, Liu F, Brown MB. Advance in oral transmucosal drug delivery, j control release. 2011; 153 (2); 106-16.
Rincón AM, Vasquez AM, Padilla FC. Chemical composition and bioactive compounds of flour of orange (citrus sinensis), tangerine (citrus reticulata), and grapefruit (citrus paradisi) peels cultivated in venezuela, archivos latinoamericanos de nutricion. 2005; 55; 305-310.
Verez B. Anti inflammatory role of natural polyphenols and their degradation products, dalam severe sepsis and septic shock:
understanding a serious killer, chapter 18. 2012; 384.
Kumar S, Pandey A. Chemistry and biological activities of flavonoids: an overview, sci world j. 2013; 12, doi 10.1155/2013/162750.
Manthey JA, Buslig BS. flavonoids in cell function. Springer, New York. 2013.
Gosslau A, Li S, Ho CT, Chen KY, Rawson NE. The importance of natural product characterization in studies of their antiinflammatory activity, mol nutr food res. 2011;
; 74-82.
Manthey JA, Bendele P. Anti-inflamatory activity of an orange peel polymethoxylated flavone, 3’,4’,5,6,7,8-heptamethoxyflavone, in the rat carrageenan/paw edema and mouse lipopolysaccharide-challenge assays, j agric food chem. 2008; 56; 9339-9403.
Miloro M, Ghali GE, Larsen PE, Waite PD. Peterson’s principles of oral and maxillofacial surgery, 3rd edition. People’s Medical
Publishing, Connecticut. 2012; 12.
Kong AT. Inflammation, oxidative stress, and cancer: dietary approaches for cancer prevention. CRC Press, Florida. 2014; 250-
Badan POM RI. Acuan Sediaan Herbal, Badan Pengawas Obat dan Makanan Republik Indonesia, Jakarta. 2007.
Hakkinen L, Uitto J, Lariava H. Cell biology of gingival wound healing, periodontology 2000. 2000; 24; 127-152.
Larjava H. Oral wound healing: cell biology and clinical management, wiley-blackwell, Oxford. 2012; 84, 106.
Rajendran R, Sivapathasundharam B. Shafer’s Textbook of Oral Pathology, 7th edition, Elsevier, New Delhi. 2012; 598.
Fathi WK. The effect of hyaluronic acid and platelet-rich plasma on soft tissue wound healing: an experimental study on rabbits, alrafidain dent j. 2012; 12 (1); 115-125.
Choi SW, Son BW, Son YS, Park YI, Lee SK, Chung MH. The wound-healing effect of a glycoprotein fraction isolated from aloe vera. Br J Dermatol. 2001; 145 (4); 535-545.
Muralighar A, Babu KS, Sankar TR, Reddana P, Latha J. Wound healing activity of flavonoid fraction isolated from the stem bark of butea monosperma (lam) in albino wistar rats. Euro J Exp Bio. 2013; 3 (6); 1-6.
Foster J. Nutrition and Wound Healing, dalam Management of Complex Wounds, Crit Care Nurs Clin North America. 2012; 24 (2).
Buch JG. Pharmacology Recap, PDU Medical College, Rajkot. 2010; 392.
Mc Culloch JM, Kloth LC. Wound Healing: Evidence Based Management, 4th edition, Davis Company, Philadelphia. 2010; 49.
Philips N, Auler S, Hugo R, Gonzales S. Beneficial regulation of matrix metalloproteinases for skin health. Enzyme Res. 2011; 2011; 1-4.
Abass EH, Mohammad TK, Zair SK, Nagem SA. Recovery of pure hesperidin from iraqi sweet orange peel and study the effect in some bacteria, baghdad science journal. 2014; 11 (2); 455-460.
DOI: https://doi.org/10.22146/majkedgiind.9012
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