Transforming Oil Palm Trunk (OPT) Waste into Black Soldier Fly (BSF) Larval Biomass: Investigating Pretreatment and Enrichment

Lienda A. Handojo; Agus Dana Permana; Made Tri Ari Penia Kresnowati; Tanto Pratondo Utomo; Giovanni Arneldi Sumampouw; Graecia Lugito; Reinhardt Anderson; Isna Dea  Fauzani; Nadya Putri Deyanti; Satya Amara; Daniel Pramudita

doi:10.22146/ajche.20345

Lienda A. Handojo Chemical Engineering, Bandung Institute of Technology, West Java, Indonesia
Agus Dana Permana School of Life Sciences and Technology, Bandung Institute of Technology, West Java, Indonesia
Made Tri Ari Penia Kresnowati Chemical Engineering, Bandung Institute of Technology, West Java, Indonesia
Tanto Pratondo Utomo Department of Agroindustrial Technology, The University of Lampung, Lampung, Indonesia
Giovanni Arneldi Sumampouw Chemical Engineering, Bandung Institute of Technology, West Java, Indonesia
Graecia Lugito Chemical Engineering, Bandung Institute of Technology, West Java, Indonesia
Reinhardt Anderson Food Engineering, Bandung Institute of Technology, West Java, Indonesia
Isna Dea Fauzani Food Engineering, Bandung Institute of Technology, West Java, Indonesia
Nadya Putri Deyanti Food Engineering, Bandung Institute of Technology, West Java, Indonesia
Satya Amara Food Engineering, Bandung Institute of Technology, West Java, Indonesia
Daniel Pramudita Chemical Engineering, Bandung Institute of Technology, West Java, Indonesia

DOI: https://doi.org/10.22146/ajche.20345

Keywords: Black Soldier Fly, Larval Growth, Oil Palm Trunk, Waste Reduction Index

Abstract

The Indonesian government's palm oil replantation program has the potential to create an excess of oil palm trunks (OPT) in the field. Although there is an effort to obtain ethanol from the sap pressed from OPT, the OPT dregs (OPTD) remain as waste. This research investigates the potential of using OPTD as both a feed and growth medium for black soldier fly (BSF) larvae, which can be subsequently processed into animal feed. This manuscript focuses on the treatment of OPTD through maceration in water and palm oil mill effluent (POME), followed by the addition of supplementary materials, namely palm kernel meal (PKM), Azolla leaves, and solid decanter, to enhance the nutritional value and digestibility of the substrate. The effectiveness of these treatments is evaluated based on their waste-reducing capacity and the BSF larval growth. Macerating OPTD in 20% POME for 4 days was found optimal for enhancing the starch and sugar contents by 17% and 11%, respectively. The addition of PKM enhanced the growth of BSF larvae the most, resulting in a higher waste reduction index (WRI). However, it was not necessarily the most effective from the perspective of biomass conversion. The results here show that the treated OPTD mixture can serve as a viable complementary growth medium for BSF larvae.

References

Andari, G., Ginting, N.M., and Nurdiana, R. 2021. “Black soldier fly larvae (Hermetia illucens) as a waste reduction agent and an alternative livestock feed.” J. Ilmiah Peternakan Terpadu 9(3), 246–252 https://doi.org/10.23960/jipt.v9i3.p246-252

Arifki, M.P., Tafsin, M., and Fatimah. 2024. “The effect of agro-industrial waste mixtures on productivity and nutrient content of black soldier fly larvae (Hermetia illucens).” J. Environ. Dev. Studies 5(1), 1-10.

Bajra, B.D., Lubis, M.E.S., Yudanto, B.G., Panjaitan, F.R., Rizki, I.F., Mulyono, M.E., and Kusumah, M.S. 2023. “Determination of black soldier fly larvae performance for oil palm-based waste reduction and biomass conversion.” J. Environ. Manage. 343, 118269. https://doi.org/10.1016/j.jenvman.2023.118269

Bajra, B.D., Lubis, M.E.S., Suprianto, E., Yudanto, B.G., Mulyono, M.E., Rizki, I.F., Kusumah, M.S., and Panjaitan, F.R. 2025. “Bioconversion of oil palm empty fruit bunch and kernel meal by black soldier fly (Hermetia illucens) as an alternative protein and fat sources.” Bioresour. Technol. Rep. 29, 102067. https://doi.org/10.1016/j.biteb.2025.102067

Belperio, S., Cattaneo, A., Nannoni, E., Sardi, L., Martelli, G., Dabbou, S., and Meneguz, M. 2024. “Assessing substrate utilization and bioconversion efficiency of black soldier fly (Hermetia illucens) larvae: effect of diet composition on growth and development temperature.” Animals 14(9), 1340. https://doi.org/10.3390/ani14091340

Benbelgacem, F. F., Bellag, O. A., Ahmad, A. A., Noorbatcha, I. A., and Salleh, H. Mohd. 2017. “Palm oil mill effluent metagenome for cellulose-degrading enzymes.” J. Basic Appl. Res. 3(3), 101–106.

BPS-Statistics Indonesia, 2024. Indonesia Palm Oil Statistics 2023, ISSN/ISBN: 1978-9947. https://www.bps.go.id/id/publication/2024/11/29/d5dcb42ab730df1be4339c34/statistik-kelapa-sawit-indonesia-2023.html

Broeckx, L. Frooninckx, L. Slegers, L., Berrens, S., Noyens, I., Goossens, S., Verheyen, G., Wuyts, A., and van Miert, S. 2021. “Growth of black soldier fly larvae reared on organic side-streams.” Sustainability 13(23), 12953. https://doi.org/10.3390/su132312953

Camperio, J., Suarez, J.A., Simonton, J., Paresky, E., Parodi, J., and Benetti, D.D. 2025. “Valorizing organic waste through black soldier fly larvae (Hermetia illucens): A sustainable solution for aquafeeds with key nutrients and natural bioactive polyphenols.” Sustainability 17(5), 1788. https://doi.org/10.3390/su17051788

Damanik, N.F., Putra, R.E., Kinasih, I., and Permana, A.D. 2023. “Growth and development performance of Hermetia illucens L. (diptera: stratiomyidae) larvae on fermented palm kernel meal (PKM) substrate.” HAYATI J. Biosci. 31(2), 317-327.

Dekker, K.D. 1950. “The Luff-Schoorl method for determination of reducing sugar in juices, molasses and sugar.” SASJ 34,157-171.

Diener, S., Zurbrügg, C., and Tockner, K. 2009. “Conversion of organic material by black soldier fly larvae: establishing optimal feeding rates.” Waste Manag. Res. 27(6), 603-610. https://doi.org/10.1177/0734242X09103838

Eggink, K. M., Donoso, I. G., and Dalsgaard, J. 2023. “Optimal dietary protein to carbohydrate ratio for black soldier fly (Hermetia illucens) larvae.” J. Insects Food Feed 9(6), 789-798. http://dx.doi.org/10.3920/JIFF2022.0102

Ganesan, A.R., Mohan, K., Kandasamy, S., Surendran, R.P., Kumar, R., Rajan, D.K., and Rajarajeswaran, J. 2024. “Food waste-derived black soldier fly (Hermetia illucens) larval resource recovery: A circular bioeconomy approach.” PSEP 184, 170-189. https://doi.org/10.1016/j.psep.2024.01.084

Guillaume, J.B., Mezdour, S., Marion-Poll, F., Terrol, C., and Schmidely, P. 2023. “Asymptotic estimated digestibility, a new indicator of black soldier fly (Hermetia illucens) conversion efficiency in relation to larval density.” J. Insects Food Feed 9(7), 1-14. https://doi.org/10.3920/JIFF2022.0103

H’ng, P.S. Wong, L.J. Chin, K.L. Tor, E.S. Tan, S.E. Tey, B.T., and Maminski, M. 2011. “Oil palm (Elaeis guineensis) trunk as a resource of starch and other sugars.” J. Appl. Sci. 11, 3053-3057. https://doi.org/10.3923/jas.2011.3053.3057

Indonesian National Standard, SNI 3547-2-2008 (2008). Kembang Gula.

Indonesian National Standard, SNI 01-2891-1992 (1992). Cara Uji Makanan dan Minuman.

Ismail, R., Trisna, A., Tati, V.S., and Amran, M. 2023. “Evaluation nutrient of larvae black soldier fly (Hermetia illucens) on fermented palm oil sludge culture media.” IOP Conf. Ser.: Earth Environ. Sci. 1286, 012021. https://doi.org/10.1088/1755-1315/1286/1/012021

Julita, U., Fitri, L.L., and Permana, A.D. 2023. “Bioconversion efficiencies of several food waste by black soldier fly, Hermetia illucens (L.) (diptera: Stratiomyidae) larvae for sustainable waste management.” The Symposium on Advance of Sustainable Engineering 2021 (SIMASE 2021): Post Covid-19 Pandemic: Challenges and Opportunities in Environment, Science, and Engineering Research. AIP Conference Proceedings 2646 (AIP Publishing, Melville, NY), pp. 020011.

Kieβling, M., Franke, K., Heinz, V., and Aganovic, K. 2023. "Relationship between substrate composition and larval weight: a simple growth model for black soldier fly larvae." J. Insects Food Feed 9(8), 1027-1036. https://doi.org/10.3920/JIFF2022.0096

Lu, S., Taethaisong, N., Meethip, W., Surakhunthod, J., Sinpru, B., Sroichak, T., Archa, P., Thongpea, S., Paengkoum, S., Purba, R. A. P., and Paengkoum, P. 2022. “Nutritional composition of black soldier fly larvae (Hermetia illucens L.) and its potential uses as alternative protein sources in animal diets: A review.” Insects 13 (9), 831. https://doi.org/10.3390/insects13090831

Lubis, M.E.S, Bajra, B.D., Rizki, I.F., Mulyono, M.E., Yudanto, B.G., and Panjaitan, F.R. 2023. “The effect of oil palm empty fruit bunch and kernel meal composition as black soldier fly larvae (Hermetia illucens) feedstock on driving the changes in larval fatty acids composition.” J. Penelitian Kelapa Sawit 31(1), 13-24.

Mokhtar, A., Siti Nadrah, A.H., Kamruddin, H., Astimar, A.A., and Mohd basri, W. 2011. “Isolation and functional properties of hemicelluloses from oil palm trunks.” J. Oil Palm Res. 23(3), 1178-1184.

Nasution, S.N., Nazir, N., Wellyalina, Azmi, A.F.M.N., and Hariry, A. 2023. “Effect of soaking time on the physicochemical characteristics, antioxidant activity, and glycemic index of parboiled local cultivar black rice from West Sumatra.” Indonesian Food Sci. Tech. J. 7(1), 56-66.

Norhazimah, A., Siti, F. M. N., Muhamad, A., Dilaeleyana, A. B., and Shahirah, M., 2020. “Direct fermentation of oil palm (Elaeis guineensis) trunk sap to Bioethanol by Saccharomyces cerevisiae.” IOP Conf. Ser.: Mater. Sci. Eng. 943, 012012. https://doi.org/10.1088/1757-899X/943/1/012012

Olawoye, B., Jolayemi, O.S., Origbemisoye, B.A., Oluwajuyitan, T.D., and Popoola-Akinola, O. 2023. Hydrolysis of Starch. In: Sharanagat, V.S., Saxena, D.C., Kumar, K., Kumar, Y. (eds). Starch: Advances in Modifications, Technologies and Applications. Springer, Cham.

Palma, L., Fernandez-Bayo, J., Niemeier, D., Pitesky, M., and VanderGheynst, J. S. 2019. “Managing high fiber food waste for the cultivation of black soldier fly larvae.” NPJ Sci. Food 3, 15. https://doi.org/10.1038/s41538-019-0047-7

Permana, A.D. and Putra J.E.N.R.E. 2018. “Growth of black soldier fly (Hermetia illucens) larvae fed on spent coffee ground.” IOP Conf. Ser.: Earth Environ. Sci. 187, 012070. https://doi.org/10.1088/1755-1315/187/1/012070

Permana, A.D., Putra, R.E., Nurulfah, A., Rosmiati, M., and Kinasih, I. 2021a. “Growth of black soldier fly larvae (Hermetia illucens) fed with pak choi (Brassica chinensis) and carp (Cyprinus carpio) residues.” Biotropia 28(2), 92- https://doi.org/101.10.11598/btb.2021.28.2.1078

Permana, A.D., Rohmatillah, D.D.F., Putra, R.E., Julita, U., and Susanto, A. 2021a. “Bioconversion of fermented barley waste by black soldier fly Hermetia illucens L. (Diptera; Stratiomyidae). J. Biodjati 6(2), 235–245.

Permana, A.D., Susanto, A., and Giffari, F.R. 2022. “Growth performance of black soldier fly larvae Hermetia illucens Linnaeus (Diptera: Stratiomyidae) in soybean skin and banana peel substrate.” (in Indonesian: “Kinerja pertumbuhan larva lalat tentara hitam Hermetia illucens Linnaeus (Diptera: Stratiomyidae) pada substrat kulit ari kedelai dan kulit pisang”).” J. Agrikultura 33(1), 13-24.

Rampure, S. and Velayudhannair, K. 2023. “Influence of agricultural wastes on larval growth phases of the black soldier fly, Hermetia illucens (Diptera: Stratiomyidae): An integrated approach.” J. Appl. Nat. Sci. 15(2), 860-869. http://dx.doi.org/10.31018/jans.v15i2.4656

Raeiszadeh, Z., Ahmadi, K., and Asadi, M. 2025. “Enhancing agricultural waste bioconversion by Black Soldier Fly larvae through wheat bran supplementation.” Int. J. Recycl. Org. Waste Agric. (in press). https://doi.org/10.57647/ijrowa.2025.16869

Soleimaninanadegani, M. and Manshad, S. 2014. “Enhancement of biodegradation of palm oil mill effluents by local isolated microorganisms.” Int. Sch. Res. Notices 2014, 727049. https://doi.org/10.1155/2014/727049

Teoh, Y.P. and Don, M.M. 2011. “Kinetic model for the hydrolysis of sterilized palm press fibre.” Chem. Eng. Sci. 66(15), 3523-3530. https://doi.org/10.1016/j.ces.2011.04.011

Wahyudi, F.T., Sumiati, and Hermana, W. 2022. “Estimation modelling of metabolizable energy in broiler ration and feedstuffs based on proximate analysis and gross energy.” J. Ilmu Nutrisi Teknologi Pakan 20(3), 104-110.

Wang, Y., Li, S., and Li, Q. 2023. "Chronological and carbohydrate dependent transformation of fatty acids in the larvae of black soldier fly following food waste treatment." Molecules 28(4), 1903. https://doi.org/10.3390/molecules28041903

Zhang, J., Luo, Z., Li, N., Yu, Y., Cai, M., Zheng, L., Zhu, F., Huang, F., Tomberlin, J.K., Rehman, K.u., Yu, Z., and Zhang, J. 2023. "Cellulose-degrading bacteria improve conversion efficiency in the co-digestion of dairy and chicken manure by black soldier fly larvae." J. Environ. Manage. 326, 116744. https://doi.org/10.1016/j.jenvman.2023.119156.