Utilization of Sugars in Hydrolysate from Oil Palm Empty Fruit Bunches for Ethanol Fermentation using Pichiastipitis CBS 5773

  • Herri Susanto Department of Chemical Engineering, Institut Teknologi Bandung
  • Achmad Ali Syamsuriputra Department of Chemical Engineering, Institut Teknologi Bandung
Keywords: cid hydrolysis, ethanol fermentation, oil palm empty fruit bunches (EFB), Pichia stipitis CBS 5773

Abstract

The utilization of pentose derived from hydrolysis of oil palm empty fruit bunches (EFB) were studied using (a) acid hydrolysis at a mild condition, (b) detoxification of hydrolysate containing pentose, (c) cell growth, and (d) fermentation to produce ethanol. Although Saccharomyces cerevisiae and Pichia stipitis were observed to be able to metabolize pentose in the hydrolysate derived from EFB, only Pichia stipitis was able to produce ethanol. When xylose was used as a model compound for pentose, the optimum xylose concentration for fermentation was 30 glL with the ethanol concentration in fermentation broth upto 13 g/L. Fermentation with higher xylose concentrations produced ethanol with a concentration of about 6 glL. Fermentation using pentose derived from detoxified hydrolysate could produce ethanol with a concentration in fermentation broth of about 3 g/L.

References

Cundasari, V., Syamsuriputra, A. A., and Susanto, H. (2000). "Preliminary experiments on the utilization of hydrolysate from oil palm empty fruit bunches for ethanol fermentation using Pichia stipitis CBS 5773" [Indonesian), Proceedings of Chemical Engineering Seminar, Surabaya, Indonesia.
Dominguez, J. M., Cao, N., Gong, C. S., and Tsao, G. T. (1999). “Ethanol production from xylose with the yeast Pichia stipitis and simulataneous product recovery by gas stripping using a gas-lift loop fermentor with attached side-arm," Biotechnology and Bioengineering, 67.
Fadjarwati, D., Rusmanto, and Susanto, H. (2000), “Optimization of hydrolysis condition of oil-palm empty fruit bunches" (in Indonesian], Proceedings of Chemical Engineering Seminar, Semarang, Indonesia.
Larsson, S., Reimann, A., Nilvebrant, N-O., and Johnson, L. J. (1999). "Comparison of different methods for the detoxification of lignocellulose hydrolysates of spruce," Applied Biochemistry and Biotechnology, 77-79.
McMillan, J. D. (1996). "Hemicellulose conversion to ethanol," Handbook of ethanol: Production and utilization.
Palmqvist, E., Barbel, H. H., Szengyel, Z., Zacchi, G., and Reczey, K. (1997). “Simultaneous detoxification and enzyme-production of hemicellulose hydrolysate obtained after steam pretreatment," Enzyme and Microbiol. Tech., 20.
Rusmanto, and Susanto, H. (2000). "Two-stage processes for fractionation of oil-palm empty fruit bunches," Proceedings of the Regional Symposium on Chemical Engineering, Singapore.
Susanto, H., and Syamsuriputra, A. A. (2003). "Development of ethanol fermentation process for the utilization of oil palm empty fruit bunches," Report for the AUN/SEED-net (Inter-University ASEANJapan Cooperation), Project No. ITB (INA) 0102
Published
2004-12-31
How to Cite
Susanto, H., & Syamsuriputra, A. A. (2004). Utilization of Sugars in Hydrolysate from Oil Palm Empty Fruit Bunches for Ethanol Fermentation using Pichiastipitis CBS 5773. ASEAN Journal of Chemical Engineering, 4(2), 40-47. Retrieved from https://journal.ugm.ac.id/v3/AJChE/article/view/7622
Section
Articles