Kinetics of Polychlorinated Biphenyl Biodegradation Using Biofilm Grown on Biphenyl

  • Josephine Borja Asian Regional Research Programme on Environmental Technology (ARRPET) National Research Institute on Industrial and Hazardous Wastes, De La Salle University
  • Joseph Auresenia Asian Regional Research Programme on Environmental Technology (ARRPET) National Research Institute on Industrial and Hazardous Wastes, De La Salle University
  • Susan M Gallardo Asian Regional Research Programme on Environmental Technology (ARRPET) National Research Institute on Industrial and Hazardous Wastes, De La Salle University
Keywords: Acclimatization, biofilm, degradation rate, kinetics, polychlorinated biphenyls (PCB), three-phase fluidized-bed reactor

Abstract

The kinetics of polychlorinated biphenyl (PCB) degradation in a completely mixed three-phase fluidized-bed biofilm reactor was studied using an initial PCB concentration of 40 ppm. The mixed-culture biofilm grown on cement balls was gradually acclimatized to PCBs prior to the experimental runs. The time course of PCB concentration was monitored and the data obtained were fitted to first, second, and third order rate equations. Analysis of data was based on the assumptions that the PCB concentration was rate limiting and the mixed liquor volatile solids (MLVS) represents the active biomass. Linear regression analysis conducted for the 11 experimental runs show that PCB degradation does not follow first order kinetics. The best fit was obtained for second order in the first six runs when the overall PCB degradation was 80-85% (8-6 ppm final concentration). When the overall degradation increased to 89-92% (4-3 ppm final concentration) from run 7 onwards, the third order gave the best fit. The improved performance of the biofilm to degrade PCBs resulted in a kinetic rate pattern, which shifted from second to third order as the concentration of the PCBs dropped. The rate of PCB degradation was influenced by the presence of mixed culture whose combined attack on and long contact with PCBs resulted in PCB degradation that progressed from one batch to the next.

References

1. Abramowicz, D. A., Brennan, M. J., Van Dort, H. M., and Gallagher E. L. (1993). "Factors influencing the rate of polychlorinated biphenyl dechlorination in Hudson River sediments," Environ. Sci. Techno!, 27, 6, 1125-1131.
2. Ahmad, D., Masse, R., Sylvestre, M., and Sandossi, M. (1991). "Bioconversion of 2-hydroxy-6-oxo-6-(4' -chlorobi- phenyl)hexa-2,4-dienoic acid: The meta-cleavage product of 4-chloro biphenyl," J. of Gen. Microbiol., 137, 1375-1385.
3. Ahmed, M., and Focht, D. (1973). "Degradation of polychlorinated biphenyls by two species of Achromobacter," Can. J. of Microbio!, 19, 42-82.
4. Alloway, B. J., and Ayres, D. C. (1997). Chemical principles of environmental pollution, 2nd ed., Blackie Academic and Professional, London.
5. Auresenia, J. (2002). Mathematical modeling and analysis of the reaction kinetics and dynamics of a three-phase fluidized-bed biofilm reactor for wastewater treatment, Ph.D. Dissertation, Waseda University, Tokyo, Japan.
6. Bedard, D., Haberl, M., May, R, and Brennan, M. (1987a). "Evidence of novel mechanisms ofpolychlorinated biphenyl metabolism in A1calegenes eutrophus H850," App! Environ. Microbiol., 53,5, 1103-1112.
7. Bedard, D., Wagner, R, Brennan, J., and Haberl, M. (1987b). "Extensive degradation of Aroclorsand environmentally transformed polychlorinated biphenyls by A1calegenes eutrophus H850," Appl. Environ. Microbio!, 53, 5, 1094-1102.
8. Borja, J., Auresenia, J., and Gallardo, S. (2004, October 24-26). Biodegradation of polychlorinated biphenyls using biofilm grown with biphenyl as carbon source in a fluidized-bed reactor. International Conference on Biofilm Structure and Activity, Las Vegas, Nevada.
9. Clark, R., Chian, E., and Griffin, R (1979). "Degradation of polychlorinated Kinetics of Polychlorinated Biphenyl Biodegradation Using Biofilm Grown on Biphenyl 51 biphenyls by mixed microbial cultures," Appl Environ.Microbiol.,37, 680-685.
10. Cookson, J. T. (1995). Bioremediation engineering: Design and application, McGraw-Hili,New York.
11. Dercova,K, Vrana, B., and Balaz,S. (1999). "A kinetic distribution model of evaporation, biosorption and biodegradation of polychlorinated biphenyls (PCBs)," Chemosphere,38, 6, 1391-1400.
12. Erickson, M. P. (1997). Analytical chemistry of NewYork. PCBs, 2nded., CRC Lewis Publishers,
13. Fava, E, Di Gioia, D., Cinti, S., Marchetti, L., and and Quatroni, dechlorination G. (1994). of low "Degradation chlorinated biphenyls by a 3-membered bacterial coculture,"Appl. Microbiol Biotechnol, 41, 117-123.
14. Focht, D., and Brunner, W. (1985)"Kinetics of biphenyl and chlorinated biphenyl metabolism in soil," Appl. Microbiol. Technol.,50, 4,1058-1063.
15. Frame,G., Cochran, J., and Boewadt, S.(1996)."Complete PCB congeners distribution for 17 Aroclor mixtures determined by 3 HRGC systems optimized for comprehensiveness, quantitative, congener-specific analysis," J. High Resolut Chromatogr.,19, 657-668.
16. Furukawa,K (1982). "Microbial degradation of polychlorinated biphenyls (PCBs)," Biodegradation and detoxification of environmental pollutants, Chakrabarty, A. M., ed., CRC Press, Boca Raton, Florida. 33-57.
17. Furukawa,K, Tomizuka,N., and Kamibayashi, A. (1983). "Metabolic breakdown of Kaneclors (polychlorinated biphenyls) and their products byAcinetobactersp.," Appl Environ.Microbiol, 46, 40-145.
18. Gamble,W. (1986). "PCB sand the environment: Perturbations of biochemical systems,"PCBs and theenvironment vol. 2, Waid,J. S., ed., CRC Press, Inc., Boca Raton, Rorida.
19. Gutierrez, R., Hirose, J., Yoshino, S., Furukawa, K., and Hayashida, S. (1995). "Distribution of bph genes encoding biphenyl/polychlorinated biphenyl degradation in soil bacteria," Research work for an international postgraduate university course in Microbiology, Kyushu University,Fukuoka, Japan.
20. Masse, R., Messier,E, Peloquin, L., Ayotte,c., and Sylvestre, M. (1984). "Microbial biodegradation of 4-chlorobiphenyl, A model compound of chlorinated biphenyls,"Appl Environ.Microbiol,47, 5, 947-951.
21. Mukerjee-Dhar, G., Shimura, M., and Kimbara, K. (1998). "Degradation of polychlorinated biphenyl by cells of Rhodococcus opacus strain TSP203 immobilized in alginate and in solution," Enzyme Microb. Techn01., 22, 34-41.
22. Parsons, J. R., and Sijm, D. T. H. M. (1988). "Biodegradation kinetics of polychlorinated biphenyls in continuous cultures of a Pseudomonas strain," Chemosphere, 17, 9, 1755-1766.
23. Safe, S. (1992). "'Toxicology,structure-function relationship, and human and environmental health impacts of polychlorinatedbiphenyls:Progressand problems," Environ. Health Perspect., 100, 229-268.
24. Sawney,B. of PCBs L. (1986). in relation "Chemistryand to environmental properties effects,"PCBs and the environment,vol. 1, Waid,J. S., ed., CRCPress,Inc.,Boca Raton, Florida.
25. Sierra, I.,Valera,J. L., and Marina,M. L. (2003). "Study of the biodegradation processof polychlorinated biphenyls in liquid medium and soil by a new isolated aerobic bacterium (Janibacter sp.)," Chemosphere, 53,609-616.
26. Simkins,S., and Alexander,M. (1984). "Models of mineralization kinetics with variable substrate concentration and population density," Appl Environ. Microbiol, 47, 6, 1299-1306.
27. Sylvestre,M. (1985). "Totalbiodegradation of4- chlorobiphenyl (PCB) by a two- membered bacterial culture," AppJ. Environ. Biotechnol., 21, 193-197.
28. Tsuneda,S., Auresenia,J., Morise,T.,and Hirata, A. (2002). "Dynamic modeling and simulationof a three-phase fluidizedbed batch process for wastewater treatment," Process Biochem., 38, 599-604.
29. Tucker,E. S., Litschgi, W. J., and Mees, W. M. (1995). "Migration of polychlorinated biphenyls in soil induced by percolating water," Bull Environ. Contam. Toxicol, 13, 1, 86-93. University of Waterloo.
30. Environmental microbiology. Available online: http:// wvlc.uwaterloo.ca/biology44 7/modules/ module7/7b4_s1.htm. Date accessed: September 2004.
31. Vagi,D., and Sudo, R. (1980). "Degradation of polychlorinated biphenyls by microorganisms," Water Pollution Control Federation, 52, 1035-1043.
Published
2006-12-31
How to Cite
Borja, J., Auresenia, J., & Gallardo, S. M. (2006). Kinetics of Polychlorinated Biphenyl Biodegradation Using Biofilm Grown on Biphenyl. ASEAN Journal of Chemical Engineering, 6(1), 44-52. Retrieved from https://journal.ugm.ac.id/v3/AJChE/article/view/7658
Issue
Vol 6 No 1 (2006)
Section
Articles

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