Study of Suspended Impurities Origin and Composition in the Treatment Process of Johkasou System

  • Yenni Trianda Chemical Engineering Department, Universitas Bung Hatta, 25147, Padang, Indonesia
  • Reni Desmiarti Chemical Engineering Department, Universitas Bung Hatta, 25147, Padang, Indonesia
  • Tomonari Fujisawa Civil Engineering Department, Gifu University, 501-1193, Gifu, Japan
  • Yasushi Ishiguro River Basin Research Center, Gifu University, 501-1193, Gifu, Japan
  • Fusheng Li River Basin Research Center, Gifu University, 501-1193, Gifu, Japan
Keywords: fine particles, flow cytometry, johkasou system, PCR-DGGE, water quality, wastewater treatment

Abstract

Johkasou system is widely used in Japan for household wastewater treatment and have played important roles in maintaining the water environment. However, out of the total units used in Gifu Prefecture, about 10% cannot meet the quality requirement for the effluent transparency above 30 degrees. Previous studies have shown clearly that the reason is mainly attributed to suspended impurities that remain in the water after treatment, with more than 96% being consisted of fine particles with sizes of 0.5-1.0 μm. A detailed investigation for identifying the origin and composition of the fine particles is necessary in order to find the approaches to improve the treatment function of the related Johkasou units. The analysis by Flow Cytometry suggested that the organic fine particles could be grouped into bacterial fraction, existed in all tanks of the Johkasou, and non-bacterial fraction that was confirmed mainly existed in anaerobic tanks, thus suggesting the lower transparency of Johkasou's effluent was greatly contributed by the bacterial fraction. PCR-DGGE further identified that some of the bacteria contained in the effluent were originated from the anaerobic tank.

References

1. Brigmon, R. L., M. Furlong, and W. B. Whitman (2003). Identification of Thiothrix unzii in two distinct ecosystem, Appl. Microbiol., 36,88-91.
2. Gaulke, L. S. (2006).Johkasou: on-site wastewater treatment and reuses in Japan, Proceedingsof the Institute of Civil Engineers -Water Mangagement: in press.
3. Hammes, F. et al. (2008). Flow-cytometric total bacterial cell counts as a descriptive microbiological parameter fordrinking water treatment processes, Water Res.,42, 269-277.
4. McGarvey, J. A. et al. (2004). Identification of bacterial populations in dairy wastewaters by use of 16S rRNA gene sequences and other genetic markers, Appl. Environ. Microbiol.,70(7),4267-4275.
5. Muyzer,G., E. C. de Waal, and A.Uitterlinden (1993).Profiling of complexmicrobial population by DGGE analysis of polymerasechain reaction-amplified genes coding for 16S rRNA,Appl.Environ. Microbiol.,62,2676–2680.
6. Oren, A. (2014). The Prokaryotes: Alphaproteobacteria and Betaproteobacteria, Springer, Berlin, Heidelberg. 975-998.
7. Panday, D. and S. K. Das (2010). Chelatococcus sambhunathii sp. nov., a moderately thermophilic alphaproteobacterium isolated from hot spring sediment, Int. J. of Syst. Evol. Micr.,60,861-865.
8. Setiyawan, A. S., T. Yamada, J. A. Fajri, and F. Li (2014). Seasonal characteristics of fecal indicators in water environment receiving effluents of decentralized wastewater treatment facilities, Journal of Jpn. Soc. Civ. Eng., 70(7), III_331-III_340.
Published
2018-12-31
How to Cite
Trianda, Y., Desmiarti, R., Fujisawa, T., Ishiguro, Y., & Li, F. (2018). Study of Suspended Impurities Origin and Composition in the Treatment Process of Johkasou System. ASEAN Journal of Chemical Engineering, 18(2), 54-61. Retrieved from https://journal.ugm.ac.id/v3/AJChE/article/view/9024
Issue
Vol 18 No 2 (2018)
Section
Articles

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