@article{Dewatisari_Nugroho_Retnaningrum_urwestri_2022, title={Antibacterial and Anti-biofilm- Forming Activity of Secondary Metabolites from Sansevieria trifasciata- Leaves Against Pseudomonas aeruginosa}, volume={33}, url={https://journal.ugm.ac.id/v3/IJP/article/view/2815}, DOI={10.22146/ijp.2815}, abstractNote={<p><em>Sansevieria trifasciata</em> is a herbaceous plant frequently used in traditional medicine with antibacterial potency against <em>Pseudomonas aeruginosa</em>. <em>P. aeruginosa </em>is a known bacterial pathogen that often becomes resistant to traditional antibiotics due to its ability to form biofilms. The objective of this research was to determine whether the ethanolic extract of <em>S. trifasciata</em> has any antibacterial or anti-biofilm-forming activity against <em>P. aeruginosa</em>. Bacterial cell damage with a potential fraction of <em>S. trifasciata</em> ethanolic extract treatment was observed by scanning electron microscopy (SEM). An Anti-biofilm assay was perfomed in 96 wells with crystal violet stain. The minimum inhibitory concentration (MIC) results revealed that at a concentration of 4 mg/mL, the potential fraction of <em>S. trifasciata</em> ethanolic could inhibit bacterial growth. At this concentration, the potential fraction of <em>S. trifasciata</em> ethanolic extract could inhibit the biofilm by 60%. The identification of compounds using gas chromatography-Mass Spectrometry (GC-MS) revealed that Neophytadiene was the main active component in the extract. These compounds have the potential to be used as therapeutic agents in the prevention of bacterial biofilms and virulence-related infectious illnesses</p&gt;}, number={1}, journal={Indonesian Journal of Pharmacy}, author={Dewatisari, Whika and Nugroho, Laurentius Hartanto and Retnaningrum, Endah and urwestri, Yekti Asih P}, year={2022}, month={Jan.}, pages={100-109} }