Antibacterial activity of ethanolic extract of Averrhoa bilimbi L. fruit against Salmonella typhi

Ni Made Riris Divayani Aristyantari; Ni Ketut Ayu Juliasih; Ni Luh Gede Sudaryati; I Nyoman Arsana

doi:10.22146/ijpther.4431

Ni Made Riris Divayani Aristyantari Department of Biology, Faculty of Information Technology and Science, Hindu University of Indonesia, East Denpasar, Bali
Ni Ketut Ayu Juliasih Department of Biology, Faculty of Information Technology and Science, Hindu University of Indonesia, East Denpasar, Bali https://orcid.org/0000-0001-9958-2888
Ni Luh Gede Sudaryati Department of Biology, Faculty of Information Technology and Science, Hindu University of Indonesia, East Denpasar, Bali https://orcid.org/0000-0003-3716-5215
I Nyoman Arsana Department of Biology, Faculty of Information Technology and Science, Hindu University of Indonesia, East Denpasar, Bali https://orcid.org/0000-0002-9017-0147

DOI: https://doi.org/10.22146/ijpther.4431

Keywords: antibacterial, A. bilimbi L., typhoid fever, traditional medicine, diffusion method

Abstract

Averrhoa bilimbi L. fruit has been used in Bali traditional medicine to treat typhoid fever. The fruit contains flavonoids and triterpenoids which are considered to have antibacterial activity. This study aimed to investigate antibacterial activity of ethanolic extract of A. bilimbi L. fruit. The ethanolic extract was prepared by maceration and antibacterial activity was determined by the disc diffusion method against Salmonella typhi cultured on Mueller hinton agar (MHA). Ciprofloxacin was used as positive control and sterile distilled water as negative control. The results showed that the ethanolic extract of A. bilimbi L. fruit at various concentrations of 25; 50; 75 and 100% have an inhibition zone diameter of 0 ± 0; 13.000 ± 1.414; 18.750 ± 1.500 and 20.250 ± 1.707 mm, respectively. In addition, ciprofloxacin at concentration of 1 mg/L has an inhibition zone diameter of 34.250 ± 1.892 mm. In conclusion, the ethanolic extract of A. bilimbi L. fruit has strong antibacterial activity against S. typhi.

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