Indonesian Journal of Biomedicine and Clinical Sciences

The activity antibiofilm of betadine leaf (Jatropha multifida Linn) juice on urinary catheters

Rada Tania — 2025-03-19

Biofilm formation can reduce the efficacy of antibiotics against pathogenic microorganisms. Flavonoids are proven to have antibiofilm activity. Betadine leaf (Jatrophas multifida Linn) contains abundant flavonoids. This study aimed to evaluate the biofilm activity of the betadine leaf juice on urinary catheters. Biofilm forming Escherichia coli was isolated from a urinary catheter. The betadine leaf juice was prepared by harvesting, washing, drying, juicing, centrifuging, and sterilizing the juice. Phytochemical analysis was performed to identify the presence of steroids, alkaloids, flavonoids, and phenols. Biofilm and antibiofilm assays were conducted by incubating thioglycolate medium with varying concentrations of the betadine leaf juice and E. coli suspension, with bacterial growth and biofilm formation assessed based on turbidity observations. Alkaloids, steroids, flavonoids, and phenolic compounds have been found in the sterilized betadine leaf juice. Bacterial growth was observed in all tubes containing different dilutions of the betadine leaf juice. However, turbidity levels increased as the juice concentration decreased. Additionally, the weight of the urinary catheter was significantly greater in tubes with lower juice concentrations (p=0.02). In conclusion, the J. multifida Linn leaf juice has potential antibiofilm activity against E. coli in urinary catheters.

The relationship between the degree of low back pain and concentration levels among RICCI Catholic High School Jakarta students during the COVID-19 pandemic

Putu Radhya Kirana Karmanita — 2025-03-19

The COVID-19 pandemic has shifted most learning activities to an online format, leading to increased sitting time and a higher risk of low back pain (LBP). Low back pain can be a factor that causes changes in concentration during learning. This study aimed to evaluate the relationship between the degree of LBP and the level of learning concentration in high school students. It was a cross-sectional study conducted at RICCI Catholic High School Jakarta, involving students from grades 10, 11, and 12. Data were collected using a questionnaire that was distributed online with Google forms. The Chi-square method for data analysis was used with a significance level of 0.05. A total of 121 students participated in this study, with 66.9% did not experience LBP, 30.6% had LBP with a slight degree of pain, and 2.5% had LBP . There were no students with severe LBP. The results for low, moderate and high concentration levels were 8.3%, 70.2%, and 21.5%, respectively. The bivariate analysis between LBP and learning concentration level using was 0.062 (p>0.05). In conclusion, there is no significant relationship between the degree of LBP and the level of concentration in high school students.

The role of bisphosphonate and bone morphogenetic proteins in congenital pseudoarthrosis of the tibia management: a literature review

Panji Sananta — 2025-03-20

Congenital pseudoarthrosis of the tibia (CPT) is a rare medical condition characterized by a congenital absence of union in the tibia, which causes the leg to flex anterolaterally. Despite the use of several surgical techniques for the treatment of CPT, there is still a significant risk of failure with surgical interventions. Recent studies have shown that bisphosphonates (BP) and bone morphogenetic proteins (BMP) can be effective adjuncts to surgical procedures, increasing the success rate of bone union and reducing the chance of re-fracture. This review aimed to evaluate the role of BP and BMP in CPT. In this review, a comprehensive literature evaluation was conducted using the PRISMA method. The databases used to search for information included PubMed, ScienceDirect, and Google Scholar for the past 10 years. Six articles were included in this review. The results showed that using pharmacological agents such as BMP and BP is considered safe for pediatric patients as an adjunctive treatment to surgery. This combination can effectively increase the speed of bone union and prevent re-fractures. The small sample size, different protocols, and different doses may have impacted the study results, which should be thoroughly explained.