Acute irritation of tetracyclic c-4-hydroxyphenylcalics [4] pyrogalolarene on skin of albino rabbits

  • Aditya Nugraha Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
  • Dwi Aris Agung Nugrahaningsih Department of Pharmacology and Therapy, Faculty of Medicine, Public Health and Nursing Universitas Gadjah Mada
  • Rul Afiyah Syarif Department of Pharmacology and Therapy, Faculty of Medicine, Public Health and Nursing Universitas Gadjah Mada
  • Mia Munawaroh Yuniyanti Department of Pharmacology and Therapy, Faculty of Medicine, Public Health and Nursing Universitas Gadjah Mada
  • Ikhsan Nur Salim Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta, Indonesia
  • Ratna Sari Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta, Indonesia
  • Jumina Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta, Indonesia
  • Eti Nurwening Sholikhah Universitas Gadjah Mada
Keywords: Pg4OH, irritation test, topical, antioxidant, UV protector

Abstract

C-4-hydroxyphenylcalix[4] pyrogalolarene (Pg4OH) is a synthetic chemical compound that shows strong antioxidant activity. It is potential to be developed as UV skin protector. For topical admission, an acute skin irritation test is being prerequisite to be fulfill. This study was conducted to investigate the irritation effect of Pg4OH on the skin. In vivo study by quasi experiment with posttest only design was carried out by employing 3 adult male albino rabbits. The back hair was shaved at 24 h before the treatment. The Pg4OH was smeared on the bare shaved-skin and left naturally within 4 h. The observation began at 24, 48 and 72 h after exposure. The erythema and edema scores were measured. Calculation by using primary irritation index (PII) was applied. Calculation was categorized with globally harmonized system (GHS) and international organization for standardization (ISO) parameters. As a result, among three rabbits, only one animal shown erythema and edema with very small size (score: 1), on 3 sites, at 72 h. Both the control site and the sample sites gained same score. Two other rabbits were obtained 0 value at each observation location. According to the PII, the observed data was obtained score 0.0093 (very light or negligible). It can be concluded that the tetracyclic compound of Pg4OH does not irritate the skin.

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Published
2020-08-30
Section
Articles