Exploration the Potency of Copper and Dyes Multi-Resistant of Indigenous Bacteria Isolated from Cikijing River, West Java

Wahyu Irawati; Reinhard Pinontoan; Triwibowo Yuwono; Indah Sofiana; Valentine Lindarto; Dwi Ningsih Susilowati

doi:10.22146/jtbb.12527

Wahyu Irawati Department of Biology, Faculty of Education, Universitas Pelita Harapan. Jl.M.H. Thamrin Boulevard 1100, Lippo Karawaci, Tangerang 15811, Banten, Indonesia https://orcid.org/0000-0002-5182-040X
Reinhard Pinontoan Department of Biology, Faculty of Science and Technology, Universitas Pelita Harapan. Jl.M.H. Thamrin Boulevard 1100, Lippo Karawaci, Tangerang 15811, Banten, Indonesia https://orcid.org/0000-0002-9245-7360
Triwibowo Yuwono Department of Agricultural Microbiology, Faculty of Agriculture, Universitas Gadjah Mada. Bulaksumur, Sleman 55281, Yogyakarta, Indonesia, Phone: 0811257379 https://orcid.org/0000-0002-0180-6132
Indah Sofiana Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development. Jl. Tentara Pelajar 3A, Bogor 16111, West Java, Indonesia https://orcid.org/0000-0003-3089-5784
Valentine Lindarto Dian Harapan Lippo Village High School, Jl. Mentawai No. 201, Lippo Karawaci, Tangerang 15138, Banten, Indonesia
Dwi Ningsih Susilowati Research Center for Horticulture, Research Organisation for Agriculture and Food, National Research and Innovation Agency. Jl. Raya Jakarta-Bogor Km. 46, Cibinong, Bogor 16911, West Java, Indonesia

DOI: https://doi.org/10.22146/jtbb.12527

Keywords: Bacteria, Cikijing River, Copper, Decolorization, Dye

Abstract

Various types of textile dye have been reported to contaminate the Cikijing River, West Java, Indonesia due to its location within the industrial region of Rancaekek District. It has been understood that certain bacterial species develop copper resistance and dye decolourisation as a mechanism of stress adaptation. The study aims at isolating and characterising copper and dye resistance as well as decolourisation ability of bacteria isolated from the Cikijing River. Copper-resistant bacteria were isolated using a series dilution method on Luria Bertani media supplemented with the addition of 1-10 mM CuSO₄. Purified bacterial isolates were then tested for copper resistance onto LB agar medium supplemented with CuSO₄ concentrations ranging from 0 mM to 20 mM and decolourisation of various dyes. A total of 59 copper-resistant bacteria were successfully isolated, nine of them showed the highest copper resistance with a MIC value from 11 mM up to 16 mM CuSO₄ and resistance to 4 types of dyes up to 700 ppm. The 16S rDNA analysis showed that the nine isolates were Klebsiella sp., Klebsiella pneumoniae, Lysinibacillus boronitolerans, Lysinibacillus fusiformis, Bacillus proteoliticus, Pseudomonas stutzeri, Klebsiella variicola, Citrobacter freundii, and Klebsiella variicola. Out of nine isolates, five were found resistant to 5 mM CuSO₄ and decolourise Methylene Blue, Congo Red, and Basic Fuchsine dyes at a maximum concentration of 700 ppm.

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