Nypa Palm Frond Degradation Utilizing Cellulolytic Lactic Acid Bacteria Isolated from Nypa Palm Worm (Namalycastis rhodochorde)
Abstract
Cellulolytic lactic acid bacteria isolated from the gastrointestinal tract of nypa palm worms, namely NrLtC2, NrLtC4, and NrLtG2, exhibited cellulase enzyme activity in vitro. Cellulose degradation ability testing began on nipa palm frond fibers with variations in LAB type treatment and starter bacteria concentration (2%, 3.5%, and 5%) for 30 days. The results showed that the NrLtG2 bacterial isolate exhibited superior degradation ability compared to the other two isolates, resulting in a decrease in cellulose content of 20.14-26.39%. In contrast, the NrLtC2 and NrLtC4 isolates demonstrated lower degradation ability, with a range of 12.50-15.63%.
References
Chesson, A., 1981. Effects of Sodium Hydroxide on cereal straws in relation to the enhanced degradation of structural polysaccharides by rumen microorganisms. Journal Science Food Agriculture, 32, pp.745-758. doi: 10.1002/jsfa.2740320802.
Dar, M.A. et al., 2021. Evaluation of cellulose degrading bacteria isolated from the gut -system of cotton bollworm (Helicoverpa armigera) and their potential values in biomass convertion. PeerJ, 9, e11254. doi: 10.7717/peerj.11254.
Datta, R., 2024. Enzymatic degradation of cellulose in soil: A review. Heliyon, 10(1), e24022. doi: 10.1016/j.heliyon.2024.e24022.
Egwuatu, T.F. & Appeh, O.G., 2018. Isolation and characterization of filter paper degrading bacteria from the guts of Coptotermes formosanus. Journal of Bioremediation and Biodegradation, 9(3), 440. doi: 10.4172/2155-6199.1000440.
Holscher, H.D., 2017. Dietary fiber and prebiotics and the gastrointestinal microbiota. Gut Microbes, 8(2), pp.172-184. doi: 10.1080/19490976.2017.1290756.
Junardi, 2008. Karakteristik morfologi dan habitat cacing nipah Namalycastis rhodochorde (Polychaeta: Nereididae: Namanereididae) di Kawasan Hutan Mangrove Estuari Sungai Kakap Kalimantan Barat. Jurnal Sains MIPA, 14(2), pp.85-89.
Junardi & Riyandi, 2020. Sintasan dan pertumbuhan larva cacing nipah Namalycastis rhodochorde (Polychaeta: Nereididae) pada budidaya dengan dua sumber pakan berbeda. Jurnal Akuakultur Rawa Indonesia, 8(2), pp.193-204. doi: 10.36706/jari.v8i2.11715
Li, H. et al., 2023. Characterization of cellulose-degrading bacteria isolated from silkworm excrement and optimization of its cellulase production. Polymers (Basel), 15(20), 4142. doi: 10.3390/polym15204142.
Nakagawa, S. et al., 2017. Microbiota in the coelomic fluid of two common coastal starfish species and characterization of an abundant Helicobacter-related taxon. Scientific Reports, 7, 8764. doi: 10.1038/s41598-017-09355-2
Nogal, A., Valdes, A.M. & Menni, C., 2021. The role of short-chain fatty acids in the interplay between gut microbiota and diet in cardio-metabolic health. Gut Microbes, 13(1), pp.1-24. doi: 10.1080/19490976.2021.1897212.
Othman, M. et al., 2017. Extractive fermentation of lactic acid in lactic acid bacteria cultivation: A Review. Frontiers in Microbiology, 8, 2285. doi: 10.3389/fmicb.2017.02285.
Setyawati, T.R., Yanti, A.H. & Kurniatuhadi, R., 2021. Identification and probiotic screening of cellulolytic bacilli isolated from fecal pellets and gastrointestinal tract of nypa worm (Namalycastis rhodochorde), West Kalimantan, Indonesia. Jurnal Biologi Indonesia, 17(2), pp.189-195. doi: 10.47349/jbi/17022021/189
Tamunaidu, P. & Saka, S., 2011. Chemical characterization of various parts of nipa palm (Nypa fruticans). Industrial Crops and Products, 34(3), pp.1423-1428. doi: 10.1016/j.indcrop.2011.04.020
Yakkou, L. et al., 2021. Identification and characterization of microbial community in the coelomic fluid of earthworm (Aporrectodea molleri). Microbiology and Biotechnology Letters, 49(3), pp.391-402. doi: 10.48022/mbl.2104.04013.
Yanti, A.H., Setyawati, T.R. & Kurniatuhadi, R., 2020. Isolation and characterization of lactic acid bacteria from fecal pellets, coelomic fluid, and gastrointestinal tract of nypa worm (Namalycastis rhodochorde) from West Kalimantan, Indonesia. Biodiversitas, 21(10), pp.4726-4731. doi: 10.13057/biodiv/d211036.
Yusnia, E.D., Gunam, I.B.W. & Antara, N.Y., 2019. Isolasi dan skrining bakteri selulolitik dari beberapa tanah hutan di Bali. Jurnal Rekayasa dan Manajemen Agroindustri, 7(1), pp.11-20. doi: 10.24843/JRMA.2019.v07.i01.p02.
Zhong, R. et al., 2022. Insights into the bacterial community compositions of peanut worm (Sipunculus nudus) and their association with the surrounding environment. Frontiers in Marine Science, 9, 1076804. doi: 10.3389/fmars.2022.1076804