Identification and Characterisation of Endophytic Bacteria in Rice Bean (Vigna umbellata)

Marjohn C. Niño; Jerome H. Ruiz; Maria Lima D. Pascual

doi:10.22146/jtbb.14798

Marjohn C. Niño College of Agriculture, Food Science, Agribusiness and Development Communication, Cebu Technological University Barili Campus Cagay, Barili, Cebu, 6036, Philippines; College of Agriculture, Northern Iloilo State University Barotac Viejo Campus, Puerto Princesa, Barotac Viejo, Iloilo, 5011, Philippines https://orcid.org/0000-0003-0405-9803
Jerome H. Ruiz College of Agriculture, Food Science, Agribusiness and Development Communication, Cebu Technological University Barili Campus Cagay, Barili, Cebu, 6036, Philippines; College of Technology, Siquijor State College, Larena, Siquijor, 6226, Philippines https://orcid.org/0000-0003-4713-9520
Maria Lima D. Pascual College of Agriculture, Food Science, Agribusiness and Development Communication Cebu Technological University Barili Campus Cagay, Barili, Cebu, 6036, Philippines https://orcid.org/0000-0002-2742-2268

DOI: https://doi.org/10.22146/jtbb.14798

Keywords: Endophytic bacteria, Indigenous crop, Plant growth-promotion, Rice bean, 16S rDNA

Abstract

Rice bean (Vigna umbellata) is an underutilised legume with unexplored endophytic microbiome. Identification and characterisation of these endophytes are critical in understanding their roles in plant’s growth, health, and productivity. In this study, twelve morphologically and biochemically distinct bacterial endophytes were isolated from rice bean roots. Sequence analysis of the 16S rDNA of the isolates revealed that members of Proteobacteria, including Stenotrophomonas sp., Shinella sp., Roseomonas sp., Pantoea dispersa, and Serratia marcescens dominated the root tissues of rice bean. The remaining isolates were found to be members of Actinobacteria, Bacteroidetes, and Firmicutes. The in vitro assays showed the potential abilities of the endophytic Stenoprophomonas sp., Shinella sp., Microbacterium gilvum, Serratia marcescens, and Bacillus qingshengii in indole acetic acid production, exopolysaccharide production, and phosphate solubilisation. Overall findings suggest diverse and potentially multifunctional endophytes in rice bean that can be incorporated into agricultural practices and crop improvement programs.

Author Biography

Marjohn C. Niño, College of Agriculture, Food Science, Agribusiness and Development Communication, Cebu Technological University Barili Campus Cagay, Barili, Cebu, 6036, Philippines; College of Agriculture, Northern Iloilo State University Barotac Viejo Campus, Puerto Princesa, Barotac Viejo, Iloilo, 5011, Philippines

College of Agriculture

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