Identification of Parathelphusa sp. in the Catchment Area of Lake Matano, South Sulawesi, Using Classical Taxonomy and DNA Barcoding

Andi Wafiq  Maulidah; Andi Aliah Hidayani; Nadiarti Nurdin  Kadir; Andi Adam  Malik; Abigail Mary  Moore

doi:10.22146/jtbb.18022

Andi Wafiq Maulidah Master Program in Fisheries Science, Hasanuddin University, Makassar 90245, Indonesia; Center of Excellence for Marine Resilience and Sustainable Development, Hasanuddin University, Makassar 90245, Indonesia https://orcid.org/0009-0002-6213-8526
Andi Aliah Hidayani Fisheries Department, Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar 90245, Indonesia; Aquatic Macrofaunal Biodiversity and Conservation Research Group, Hasanuddin University, Makassar 90245, Indonesia https://orcid.org/0000-0002-4541-5539
Nadiarti Nurdin Kadir Fisheries Department, Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar 90245, Indonesia; Aquatic Macrofaunal Biodiversity and Conservation Research Group, Hasanuddin University, Makassar 90245, Indonesia; Center of Excellence for Marine Resilience and Sustainable Development, Hasanuddin University, Makassar 90245, Indonesia; EAFM Learning Centre, Universitas Hasanuddin, Makassar 90245 https://orcid.org/0009-0002-6213-8526
Andi Adam Malik Faculty of Agriculture, Animal Husbandry and Fisheries, Universitas Muhammadiyah Parepare, Parepare 91131, Indonesia https://orcid.org/0000-0003-2487-2770
Abigail Mary Moore Graduate School, Hasanuddin University, Makassar 90245, Indonesia; Aquatic Macrofaunal Biodiversity and Conservation Research Group, Hasanuddin University, Makassar 90245, Indonesia; Center of Excellence for Marine Resilience and Sustainable Development, Hasanuddin University, Makassar 90245, Indonesia; EAFM Learning Centre, Universitas Hasanuddin, Makassar 90245 https://orcid.org/0000-0002-4122-3740

DOI: https://doi.org/10.22146/jtbb.18022

Keywords: Ancient Lake Malili, Freshwater Crustaceans, Morphology, Phylogenetics, Watersheds

Abstract

The study aimed to identify and classify Parathelphusa sp. in Lake Matano using classical taxonomy and DNA barcoding. It included morphological and morphometric analysis, alongside molecular analysis with the Cytochrome Oxidase Subunit I (COI) gene and NCBI GenBank BLAST Nukleotida Standard. The results revealed two morphotypes, one identified as Parathelphusa pantherine, while the second remained unidentified despite resembling Parathelphusa pallida. This research underscores the value of combining DNA barcoding and classical taxonomy (morphological and morphometric) for species identification, offering new insights into the genetic diversity of Parathelphusa crabs in Lake Matano, supports conservation, ecosystem management, and protection of endemic species.

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