Edamame (Glycine max (L.) Merr) Seedling Growth Promotion by Streptomyces sp. RT52 and Analysis of Its phoD Gene

Ira Erdiandini; Aris Tjahjoleksono; Rika Indri Astuti; Edi Husen; Aris Tri Wahyudi

doi:10.22146/jtbb.19141

Ira Erdiandini Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, West Java 16680, Indonesia; Department of Agrotechnology, Faculty of Agriculture, Universitas Tanjungpura, West Kalimantan 78124, Indonesia https://orcid.org/0000-0002-4458-1631
Aris Tjahjoleksono IPB UniversityDepartment of Biology, Faculty of Mathematics and Natural Sciences, IPB University, West Java 16680, Indonesia https://orcid.org/0000-0001-6695-1245
Rika Indri Astuti Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, West Java 16680, Indonesia https://orcid.org/0000-0003-1561-6943
Edi Husen Research Centre for Applied Microbiology, National Research and Innovation Agency Republic Indonesia, Jl. Raya Jakarta-Bogor Km 46, Cibinong, Bogor 16911, West Java, Indonesia https://orcid.org/0000-0001-7247-3315
Aris Tri Wahyudi Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, West Java 16680, Indonesia https://orcid.org/0000-0001-7837-9557

DOI: https://doi.org/10.22146/jtbb.19141

Keywords: Edamame, Peatland, phoD gene, Plant growth-promoting, Rhizobacteria, Streptomyces

Abstract

The excessive use of synthetic fertilisers in edamame (Glycine max (L.) Merr) cultivation has raised environmental and health concerns, necessitating sustainable alternatives. This study aimed to characterise the plant growth-promoting properties of RT52 strain, an isolate from acidic peat soil, by assessing its indole acetic acid production, phosphate solubilisation activity, nitrogenase activity, in vivo seedling growth-promotion and analysis of phoD partial sequences. Based on the results of molecular identification of the 16S rRNA gene, strain RT52 was closely related to the genus Streptomyces. Interestingly, seedling growth-promotion revealed that Streptomyces sp. RT52 could significantly increase primary root length 104.64 %, shoot length 29.69 %, number of lateral roots 63.35 % and dry weight 18.75 % of edamame sprouts. Colorimetric assays confirmed that Streptomyces sp. RT52 produced 30.73 µg mL⁻¹ IAA. Its phosphate solubilisation reached 153.50 ± 23.57 µg mL⁻¹, while nitrogenase activity, determined via acetylene reduction assay, was 21.29 ± 1.04 nmol C₂H₄ h⁻¹ tube⁻¹. Nonetheless, partial sequence of PhoD Streptomyces sp. RT52 reached 99.19 % similarity with alkaline phosphatase from Streptomyces. Superposition analysis of this sequence confirmed its structural similarity to alkaline phosphatase D from Bacillus subtilis (2YEQ), supporting its role in phosphate solubilisation under acidic conditions. These findings demonstrate the potential of Streptomyces sp. RT52 as a biofertiliser candidate for promoting edamame growth.

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