The Effects of Rearing Media on the Growth and Microbiome Diversity in the Digestive Tract of Black Soldier Fly (Hermetia illucens) Larvae

Senny  Helmiati; Nur Indah  Septriani; Tiara Putri  Faralovrisya; Julian Ransangan

doi:10.22146/jtbb.19562

Senny Helmiati Fisheries Department, Faculty of Agriculture Universitas Gadjah Mada, A-4 Building, Flora Street, Bulaksumur Yogyakarta Indonesia 55281 https://orcid.org/0009-0008-0167-4433
Nur Indah Septriani Faculty of Biology, Universitas Gadjah Mada, Teknika Selatan Street, Sekip Utara, Yogyakarta Indonesia https://orcid.org/0000-0002-2159-5520
Tiara Putri Faralovrisya Fisheries Department, Faculty of Agriculture Universitas Gadjah Mada, A-4 Building, Flora Street, Bulaksumur Yogyakarta Indonesia 55281
Julian Ransangan Higher Institution Centre of Excellent (HICoE), Borneo Maritime Research Institute, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu, Sabah, 88400, Malaysia https://orcid.org/0000-0002-1369-3024

DOI: https://doi.org/10.22146/jtbb.19562

Keywords: Black Soldier Fly Larvae, Growth, Microbiome, Substrate

Abstract

This study aimed to determine the nutritional content and rearing substrate and identify types of microbiota in the digestive tracts of BSF larvae. The substrates used include BSFB (fruit waste), BSFC (fermented bread waste), BSFP (bread waste), BSFS (palm kernel meal), and BSFSO (organic waste). Data on the nutritional content of the substrates and body, the length of the intestinal epithelium of BSF larvae, and the diversity of 32 microbiomes in the digestive tract of BSF larvae were analyzed statistically using analysis of variance with a significance level of 95%. The high and low nutrient content of BSF larvae is influenced by the substrate used. BSF larvae utilized the protein in the substrate to form their body proteins. BSF larvae grown on BSFS substrate showed the highest increase in length of digestive tract epithelium compared to other substrates. Differences in substrate types are one of the factors that affect the diversity of bacterial communities. The most dominant phylum in BSFB, BSFC, BSFS and BSFSO was Proteobacteria, with relative abundances of 62.53 %, 59.63 %, 56.35 %, and 61.35 %, respectively. The most dominant genus in BSFP was Dysgonomonas (69.04 %). Differences in substrate type are one of the factors influencing the diversity of bacterial communities within the digestive tract of BSF larvae. These results provide information for formulating specific substrates that promote beneficial gut bacteria, optimize nutrient conversion, and reduce rearing costs.

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