The Effect of 2-iP (2-isopentenyl adenine) Concentration on the Growth of Ki Aksara Orchids (Macodes petola (Blume) Lindl.) In Vitro

Yenisbar; Arief  Wicaksono; Edhi  Sandra

doi:10.22146/jtbb.20733

Yenisbar Agrotechnology Study Program, Faculty of Biology and Agriculture, National University, Jl. Sawo Manila No.61 South Jakarta 12520, Indonesia
Arief Wicaksono Agrotechnology Study Program, Faculty of Biology and Agriculture, National University, Jl. Sawo Manila No.61 South Jakarta 12520, Indonesia
Edhi Sandra Department of Forest Resource Conservation and Ecotourism, Faculty of Forestry and Environment, IPB University, Jl. Raya Dramaga Kampus IPB Bogor 16680, Indonesia

DOI: https://doi.org/10.22146/jtbb.20733

Keywords: 2-isopentenyl adenine, Cytokinin, Ki Aksara Orchids, Orchids, Tissue Culture

Abstract

This study aims to determine the optimal concentration of 2-iP (2-isopentenyl adenine) for the in vitro shoot growth of Ki Aksara orchids (Macodes petola (Blume) Lindl.). The experiment was conducted at the Esha Flora Tissue Culture Laboratory, Kedung Waringin Sub-district, Tanah Sereal District, Bogor City, from December 2022 to March 2023. The method used a Completely Randomized Design (CRD) comprising six treatment levels, leading to a total of 30 experimental units. The treatment levels included P0 (Control), P1 (0.05 mg L^-1), P2 (0.10 mg L^-1), P3 (0.15 mg L^-1), P4 (0.20 mg L^-1), and P5 (0.25 mg L^-1). The observed parameters included the number of shoots, stem diameter, number of leaves, number of roots, plant height, and leaf colour. Subsequently, the collected data were analysed using Analysis of Variance (ANOVA), followed by a Least Significant Difference (LSD) test at a 95 % confidence level. The results showed that 2-iP concentration of 0.10 mg L^-1was the most effective in promoting the growth of apical shoots, stem diameter, root number, and plant height of Ki Aksara orchids. The two types of shoots observed were apical and lateral. However, the expected growth corresponded to apical shoots, which were predominantly found at 2-iP concentration of 0.10 mg L^-1.

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