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* Corresponding Author
Analyzing Antioxidant Properties of Caulerpa racemosa Harvested in Jepara Using ABTS and β-Carotene Bleaching Methods
https://doi.org/10.22146/mot.106166 Ariyanti Ariyanti(1), Akhmad Kharis Nugroho(2*), Triana Hertiani(3), Adhyatmika Adhyatmika(4), Eka Noviana(5)
(1) Doctoral Program in Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sleman, Special Region of Yogyakarta; Faculty of Health, Pharmacy Study Program of Pharmaceutical Sciences, Ngudi Waluyo University Ungaran, Semarang, Central Java
(2) Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Gadjah Mada, Sleman, Special Region of Yogyakarta
(3) Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Sleman, Special Region of Yogyakarta
(4) Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Gadjah Mada, Sleman, Special Region of Yogyakarta
(5) Department of Bioanalytical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sleman, Special Region of Yogyakarta
(*) Corresponding Author
Abstract
Caulerpa racemosa (Forsskal) J. Agardh, commonly known as sea grapes, is a green macroalga traditionally consumed as food. While previous studies have examined the antioxidant activity and chemical constituents of C. racemosa from various coastal regions, the species from Jepara, Indonesia, an ecologically distinct marine area, remains largely underexplored. This study aims to evaluate the antioxidant activity of C. racemosa ethanol extract using ABTS and β-carotene bleaching (BCB) assays, characterize its chemical profile through thin-layer chromatography (TLC) and Fourier-transform infrared (FT-IR) spectroscopy, and quantify the caulerpin content via high-performance liquid chromatography (HPLC). Extraction was performed with 96% ethanol, and phytochemical screening on TLC employed spray reagents: Dragendorff’s for alkaloids, anisaldehyde for terpenoids/steroids, and FeCl₃ for phenolics and flavonoids. The phytochemical characterization confirmed the presence of alkaloid compounds. The extract exhibited moderate antioxidant activity, with IC₅₀ values of 57.05 ppm (ABTS) and 63.75 ppm (BCB), compared to quercetin (11.87 ppm, ABTS) and BHT (26.35 ppm, BCB). Caulerpin content in the 1000 ppm extract was 26.86 ppm. These findings indicate that C. racemosa from Jepara is a promising source of natural antioxidant compounds with potential pharmaceutical applications and warrants further investigation.
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amin Hussen, N. H., Abdulla, S. K., Ali, N. M., Ahmed, V. A., Hasan, A. H., & Qadir, E. E. (2025). Role of antioxidants in skin aging and the molecular mechanism of ROS: A comprehensive review. Aspects of Molecular Medicine, 100063.
Apraj, V. D., & Pandita, N. S. (2016). Evaluation of skin anti-aging potential of Citrus reticulata blanco peel. Pharmacognosy Research, 8(3), 160–168. https://doi.org/10.4103/0974-8490.182913
Aroyehun, A. Q. B., Razak, S. A., Palaniveloo, K., Nagappan, T., Rahmah, N. S. N., Jin, G. W., Chellappan, D. K., Chellian, J., & Kunnath, A. P. (2020). Bioprospecting cultivated tropical green algae, caulerpa racemosa: A perspective on nutritional properties, antioxidative capacity and anti-diabetic potential. Foods, 9(9). https://doi.org/10.3390/foods9091313
Assalve, G., Lunetti, P., Fai, A., Terlizzi, A., Zara, V., & Ferramosca, A. (2025). Marine Algal Metabolites as Cellular Antioxidants: A Study of Caulerpin and Caulerpinic Acid in Saccharomyces cerevisiae. Marine Drugs, 23(9), 338.
Atanu, F. O., Ikeojukwu, A., Owolabi, P. A., & Avwioroko, O. J. (2022). Evaluation of chemical composition, in vitro antioxidant, and antidiabetic activities of solvent extracts of Irvingia gabonensis leaves. Heliyon, 8(7), e09922. https://doi.org/10.1016/j.heliyon.2022.e09922
Barzkar, N., Ivanova, S., Sukhikh, S., Malkov, D., Noskova, S., & Babich, O. (2024). Phenolic compounds of brown algae. Food Bioscience, 62, 105374. https://doi.org/10.1016/j.fbio.2024.105374
Bitwell, C., Indra, S. S., Luke, C., & Kakoma, M. K. (2023). A review of modern and conventional extraction techniques and their applications for extracting phytochemicals from plants. Scientific African, 19, e01585.
Bo, G., Shanshan, W., Qing, Z., Bo, P., & Yan, Z. (2024). Empowering Medical Data Analysis: An Advanced Deep Fusion Model for Sorting Medicine Document. IEEE Access, 12(January), 1650–1659. https://doi.org/10.1109/ACCESS.2023.3347029
Brglez Mojzer, E., Knez Hrnčič, M., Škerget, M., Knez, Ž., & Bren, U. (2016). Polyphenols: Extraction Methods, Antioxidative Action, Bioavailability and Anticarcinogenic Effects. Molecules (Basel, Switzerland), 21(7). https://doi.org/10.3390/molecules21070901
Cano, A., Maestre, A. B., Hernández-Ruiz, J., & Arnao, M. B. (2023). ABTS/TAC Methodology: Main Milestones and Recent Applications. Processes, 11(1). https://doi.org/10.3390/pr11010185
Chandimali, N., Bak, S. G., Park, E. H., Lim, H.-J., Won, Y.-S., Kim, E.-K., Park, S.-I., & Lee, S. J. (2025). Free radicals and their impact on health and antioxidant defenses: A review. Cell Death Discovery, 11(1), 19.
Chen, X., Shang, S., Yan, F., Jiang, H., Zhao, G., Tian, S., Chen, R., Chen, D., & Dang, Y. (2023). Antioxidant Activities of Essential Oils and Their Major Components in Scavenging Free Radicals, Inhibiting Lipid Oxidation and Reducing Cellular Oxidative Stress. Molecules, 28(11), 1–10. https://doi.org/10.3390/molecules28114559
Dhevika, S., & Deivasigamani, B. (2018). Phytochemical Profiling and Gc-Ms Analysis of Caulerpa Racemosa. Research Journal of Life Science, Bioinformatics, Pharmaceutical and Chemical Sciences, 4(5), 155–165. https://doi.org/10.26479/2018.0405.12
Fatima, I., Safdar, N., Akhtar, W., Ayaz, A., Ali, S., Elansary, H. O., Moussa, I. M., & Zaman, W. (2024). Green solvent-based extraction of three Fabaceae species: A potential antioxidant, anti-diabetic, and anti-leishmanial agents. Heliyon, 10(13), e33668. https://doi.org/10.1016/j.heliyon.2024.e33668
Fernando, I. P. S., Sanjeewa, K. K. A., Samarakoon, K. W., Lee, W. W., Kim, H.-S., & Jeon, Y.-J. (2018). Squalene isolated from marine macroalgae Caulerpa racemosa and its potent antioxidant and anti-inflammatory activities. Journal of Food Biochemistry, 42(5), e12628. https://doi.org/10.1111/jfbc.12628
Gopi, J., Kumar, S., Umamaheswari, S., Kavimani, S., Ilavarasan, R., & Murti, S. (2019). Pharmacological Potential of Green Algae Caulerpa: A Review. International Journal of Pharmaceutical Sciences and Research, 10(3), 1014. https://doi.org/10.13040/IJPSR.0975-8232.10(3).1014-24
Idris, L., Adli, M. A., Yaacop, N. N., & Zohdi, R. M. (2023). Phytochemical Screening and Antioxidant Activities of Geniotrigona thoracica Propolis Extracts Derived from Different Locations in Malaysia. Malaysian Journal of Fundamental and Applied Sciences, 19(6), 1023–1032. https://doi.org/10.11113/mjfas.v19n6.3128
Jha, A. K., & Sit, N. (2022). Extraction of bioactive compounds from plant materials using combination of various novel methods: A review. Trends in Food Science & Technology, 119, 579–591.
Jomova, K., Raptova, R., Alomar, S. Y., Alwasel, S. H., Nepovimova, E., Kuca, K., & Valko, M. (2023). Reactive oxygen species, toxicity, oxidative stress, and antioxidants: Chronic diseases and aging. In Archives of Toxicology (Vol. 97, Issue 10). Springer Berlin Heidelberg. https://doi.org/10.1007/s00204-023-03562-9
Kang, J., Kwak, Y. S., Kim, E. jeong, Gwon, Y., Choi, H. G., & Eyun, S. il. (2024). Transcriptome and functional analyses of phenotypic plasticity in sea grape Caulerpa okamurae. Physiologia Plantarum, 176(3), 1–11. https://doi.org/10.1111/ppl.14339
Keke, C. O., Nsofor, W. N., Kumabia, F. K. R., Iloabuchi, G. C., Ejiofor, J. C., & Osuagwu, O. L. (2023). GCMS and FTIR analysis of ethanol and methanol leave extract of Urena lobata (Caesar weed) for bioactive phytochemical constituents. Journal of Drug Delivery and Therapeutics, 13(1), 99–115. https://doi.org/10.22270/jddt.v13i1.5722
Khalid, S., Arshad, M., Mahmood, S., Ahmed, W., Siddique, F., Khalid, W., Zarlasht, M., Asar, T. O., & Hassan, F. A. M. (2023). Nutritional and phytochemical screening of Moringa oleifera leaf powder in aqueous and ethanol extract. International Journal of Food Properties, 26(1), 2338–2348. https://doi.org/10.1080/10942912.2023.2246685
Krishna, K. V. V. S., Venkatesh, B., Madhavi, A., Padmavathi Devi, B. V., Jaldi, C. E., & Bhargavi, D. S. (2019). Evaluation of antioxidant activity of panchachurnam: An ayurvedic polyherbomineral formulation. Research Journal of Pharmacy and Technology, 12(10), 4841–4847. https://doi.org/10.5958/0974-360X.2019.00838.2
Lee, D. S., Lim, M. S., Kwan, S. S., Kim, S. Y., & Park, S. N. (2012). Antioxidative activity and componential analysis of chamaecyparis obtusa leaf extract. Applied Chemistry for Engineering, 23(1), 93–99.
Lim, J. R., Chua, L. S., & Soo, J. (2023). Study of stingless bee (Heterotrigona itama) propolis using LC-MS/MS and TGA-FTIR. Applied Food Research, 3(1). https://doi.org/10.1016/j.afres.2022.100252
Maldonado-Sanabria, L. A., Rodriguez-Saavedra, I. N., Reyes-Peña, I. V., Castillo-Aguirre, A., Maldonado, M., Crespo, A., & Esteso, M. A. (2024). Comparative Study of the Antioxidant Activity of the Conformers of C-tetra (4-methoxyphenyl) calix [4] resorcinarene. International Journal of Molecular Sciences, 25(18), 10010.
Martemucci, G., Costagliola, C., Mariano, M., D’andrea, L., Napolitano, P., & D’Alessandro, A. G. (2022). Free radical properties, source and targets, antioxidant consumption and health. Oxygen, 2(2), 48–78.
Miller, N. J., & Rice-Evans, C.A. (1997). Factors influencing the antioxidant activity determined by the ABTS radical cation assay. Free Radical Research, 26(1), 195-199. https://doi.org/10.3109/10715769709013855.
Mungwari, C. P., King’ondu, C. K., Sigauke, P., & Obadele, B. A. (2024). Conventional and modern techniques for bioactive compounds recovery from plants. Scientific African, e02509.
Munteanu, I. G., & Apetrei, C. (2021). Analytical methods used in determining antioxidant activity: A review. International Journal of Molecular Sciences, 22(7). https://doi.org/10.3390/ijms22073380
Olugbami, J. O., Gbadegesin, M. A., & Odunola, O. A. (2014). In vitro evaluation of the antioxidant potential, phenolic and flavonoid contents of the stem bark ethanol extract of Anogeissus leiocarpus. African Journal of Medicine and Medical Sciences, 43(Suppl 1), 101–109.
Orphanides, A., Goulas, V., & Gekas, V. (2016). Drying Technologies: Vehicle to High-Quality Herbs. Food Engineering Reviews, 8(2), 164–180.
Palaniyappan, S., Sridhar, A., Kari, Z. A., Téllez-Isaías, G., & Ramasamy, T. (2023). Evaluation of Phytochemical Screening, Pigment Content, In Vitro Antioxidant, Antibacterial Potential and GC-MS Metabolite Profiling of Green Seaweed Caulerpa racemosa. Marine Drugs, 21(5). https://doi.org/10.3390/md21050278
Papaefthimiou, M., Kontou, P. I., Bagos, P. G., & Braliou, G. G. (2024). Integration of Antioxidant Activity Assays Data of Stevia Leaf Extracts: A Systematic Review and Meta-Analysis. Antioxidants, 13(6). https://doi.org/10.3390/antiox13060692
Parsa, M., Nourani, M., Baghdadi, M., Hosseinzadeh, M., & Pejman, M. (2019). Biochars derived from marine macroalgae as a mesoporous by-product of hydrothermal liquefaction process: Characterization and application in wastewater treatment. Journal of Water Process Engineering, 32(September), 100942. https://doi.org/10.1016/j.jwpe.2019.100942
Patle, T. K., Shrivas, K., Kurrey, R., Upadhyay, S., Jangde, R., & Chauhan, R. (2020). Phytochemical screening and determination of phenolics and flavonoids in Dillenia pentagyna using UV–vis and FTIR spectroscopy. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 242, 118717. https://doi.org/10.1016/j.saa.2020.118717
Pereira, D. T., Korbee, N., Vega, J., & Figueroa, F. L. (2024). The Role of Nitrate Supply in Bioactive Compound Synthesis and Antioxidant Activity in the Cultivation of Porphyra linearis (Rhodophyta, Bangiales) for Future Cosmeceutical and Bioremediation Applications. Marine Drugs, 22(5). https://doi.org/10.3390/md22050222
Qi, W., Qi, W., Xiong, D., & Long, M. (2022). Quercetin: Its antioxidant mechanism, antibacterial properties and potential application in prevention and control of toxipathy. Molecules, 27(19), 6545.
Rudrapal, M., Khairnar, S. J., Khan, J., Dukhyil, A. B., Ansari, M. A., Alomary, M. N., Alshabrmi, F. M., Palai, S., Deb, P. K., & Devi, R. (2022). Dietary polyphenols and their role in oxidative stress-induced human diseases: Insights into protective effects, antioxidant potentials and mechanism (s) of action. Frontiers in Pharmacology, 13, 806470.
Salihi, A. S. A., & Alsaadi, S. A. A. (2025). Effect of Spirulina Algae Powder and Folic Acid on Some Physiological Parameters During the Early Stages of Local ewe’s Pregnancy in the Summer. Egyptian Journal of Veterinary Science(Egypt), 56(7), 1507–1512. https://doi.org/10.21608/EJVS.2024.283832.2019
Sangeetha, S., Meenakshi, S., Akshaya, S., Vadivel, V., & Brindha, P. (2015). Antioxidant activity of different solvent extracts of Jacaranda mimosifolia D. Don bark and leaf. International Journal of Current Pharmaceutical Review and Research, 6(5), 215–221.
Shaheen, G., Ashfaq, A., Khawar, A., Jamil, Q. A., Parveen, R., Hadi, F., Ghauri, A. O., Shirazi, J. H., Asif, H. M., Shamim, T., Sumreen, L., Ali, T., Akram, M., Noor, R., Mehmood, A., & Sajid, F. (2022). Fourier transform infrared spectrometer analysis and antimicrobial screening of ethanolic extract of Operculina terpathum from cholistan desert. Pharmacy Practice, 20(2). https://doi.org/10.18549/PharmPract.2022.2.2647
Shen, N., Wang, T., Gan, Q., Liu, S., Wang, L., & Jin, B. (2022). Plant flavonoids: Classification, distribution, biosynthesis, and antioxidant activity. Food Chemistry, 383(February), 132531. https://doi.org/10.1016/j.foodchem.2022.132531
Sommer, J., Kunzmann, A., Stuthmann, L. E., & Springer, K. (2022). The antioxidative potential of sea grapes (Caulerpa lentillifera, Chlorophyta) can be triggered by light to reach comparable values of pomegranate and other highly nutritious fruits. Plant Physiology Reports, 27(1), 186–191. https://doi.org/10.1007/s40502-021-00637-6
Soumya, K., Haridas, K. R., James, J., Sameer Kumar, V. B., Edatt, L., & Sudheesh, S. (2019). Study of In vitro antioxidant and DNA damage protection activity of a novel luteolin derivative isolated from Terminalia chebula. Journal of Taibah University for Science, 13(1), 755–763. https://doi.org/10.1080/16583655.2019.1630892
Souza, C. R. M., Bezerra, W. P., & Souto, J. T. (2020). Marine alkaloids with anti-inflammatory activity: Current knowledge and future perspectives. Marine Drugs, 18(3). https://doi.org/10.3390/md18030147
Takahama, U. (1985). Inhibition of lipoxygenase-dependent lipid peroxidation by quercetin: Mechanism of antioxidative function. Phytochemistry, 24(7), 1443–1446. https://doi.org/10.1016/S0031-9422(00)81040-7
Tarman, K., Madduppa, H., & Januar, H. I. (2018). Metabolite profiles and antioxidant activity of Caulerpa racemosa with different handlings. Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, 13(3), 93–100.
Taslim, N. A., Hardinsyah, H., Radu, S., Mayulu, N., Tsopmo, A., Kurniawan, R., Tallei, T. E., Herlina, T., Maksum, I. P., & Nurkolis, F. (2024). Functional food candidate from Indonesian green algae Caulerpa racemosa (Försskal) J. Agardh by two extraction methods: Metabolite profile, antioxidant activity, and cytotoxic properties. Journal of Agriculture and Food Research, 18, 101513.
Vaghela, J. S., & Sisodia, S. S. (2011). In vitro antioxidant activity of Terminalia chebula fruit extracts. Research Journal of Pharmacy and Technology, 4(12), 1835–1843.
Wiraguna, A. A. G. P., Pangkahila, W., & Astawa, I. N. M. (2018). Antioxidant properties of topical Caulerpa sp. Extract on UVB-induced photoaging in mice. Dermatology Reports, 10(2), 7597.
Yap, V. L., Tan, L. F., Rajagopal, M., Wiart, C., Selvaraja, M., Leong, M. Y., & Tan, P. L. (2023). Evaluation of phytochemicals and antioxidant potential of a new polyherbal formulation TC-16: Additive, synergistic or antagonistic? BMC Complementary Medicine and Therapies, 23(1), 1–10. https://doi.org/10.1186/s12906-023-03921-0
Yehye, W. A., Rahman, N. A., Ariffin, A., Abd Hamid, S. B., Alhadi, A. A., Kadir, F. A., & Yaeghoobi, M. (2015). Understanding the chemistry behind the antioxidant activities of butylated hydroxytoluene (BHT): A review. European Journal of Medicinal Chemistry, 101, 295–312.
Zam, W., Ali, A., & Husein, F. (2020). Extraction of polyphenols from oregano and thyme by Maceration using glycerine. Research Journal of Pharmacy and Technology, 13(6), 2699–2702. https://doi.org/10.5958/0974-360X.2020.00480.1
Zhang, Y., Li, Y., Quan, Z., Xiao, P., & Duan, J.-A. (2024). New insights into antioxidant peptides: An overview of efficient screening, evaluation models, molecular mechanisms, and applications. Antioxidants, 13(2), 203.
Zhang, Y., & Wang, W. (2016). Antioxidant activities of various extracts from Polygonum cuspidatum in ABTS assay. Journal of Medicinal Food, 19(10), 918-924.
DOI: https://doi.org/10.22146/mot.106166
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