DRYING TEMPERATURE EFFECT OF GRANULE CONTAINING OIL COMBINATION OF LEMONGRASS-KAFFIR LIME LEAVES ON TOTAL LOSS OF OIL AND LARVICIDE ACTIVITIES

https://doi.org/10.22146/tradmedj.10693

Sri Mulyani(1*), Annisa Khoiri Z.(2), M. Syafii Nusri(3)

(1) Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara 55281, Yogyakarta, Indonesia
(2) Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara 55281, Yogyakarta, Indonesia
(3) Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara 55281, Yogyakarta, Indonesia
(*) Corresponding Author

Abstract


Granules of lemongrass and kaffir lime oil are reported to have larvicidal activity against the mosquito Aedes aegypti, with LC50 of 38.30ppm and 39.58ppm, while LC90 of 51.57ppm and 79.43ppm respectively. During the manufacture of granules, loss of oil are reported by 64.20% to 65.91% for lemongrass and kaffir lime oil. This research aims to make granules combination from lemongrass and kaffir lime oils by varying the temperature of the drying granules and testing its larvicidal activity against Ae. aegypti. Oil combination is selected by using analysis simplex lattice design, and the combination chosen is made of granules, in drying room temperature for 24h, a temperature of 50°C for 2h 30min and a temperature of 70°C for 1h. Larvicidal activity testing against larvae of Ae. aegypti is done by using the third instar larvae 20 for each granule solution made in 5 series of concentration, and left exposed for 24h. The number of deaths of larvae is calculated and analyzed by modified probit analysis Finney to determine LC50 and LC90. The results showed the combination lemongrass-kaffir lime oil is selected ratio of 9:1, and drying the granules with a temperature of 70°C for 1h produces the greatest larvicidal activity with 63.17ppm LC50 and LC90 of 85.04ppm.


Keywords


combination of oils, granules, larvicides, Aedes aegypti

Full Text:

PDF


References

Azah, M.A.N., Zaridah, M.Z., Mailina, J., Husni, S.S., Majid, J.A., Said, A.A., Faridz, Z.M., Yasmin, N.Y.M., & Rohani, A. 2006. Evaluation of The Chemical and Biological Properties of Selected Essential Oils Toward The Development of Mosquito Repellent Products, http://i nfo.frim.gov.my/cfdocs /infocenter /highlight/IRPA_2006/11.pdf, diakses pada 9 Desember 2014.

BSN (Badan Standardisasi Nasional) 1998. SNI 06- 3953-1995, http://www.scribd.com/doc

/SNI-06-1312-1998-Minyak

Sereh Dapur, diakses 15 April 2015.

Costa, J.G., Rodrigues, F.F.G., & Angelico, E.C. 2005. Chemical biological study of the

essential oils of Hyptis martiusii, Lippia sidoides and Syzigium aromaticum Againts Larvae of Aedes aegypti and Culex quinquefasciatus. Braz. J. Pharmacogn. 15: 304 -30 9.

Finney, D. J. 1971. Probit Analysis, 3 rd ed. Cambridge University

Press, Great Britain. pp 333-340.

Istianah, M.A., Utami, W.S., & Ameliana, L. 2013. Efektivitas biolarvasida minyak daun jeruk purut (Citrus hystrix) terhadap larva instar III nyamuk Aedes aegypti, Laporan Penelitian, Fakultas Kedokteran Universitas Jember, Jember.

Ketaren, S., 1985. Pengantar Teknologi Minyak Atsiri, PN Balai Pustaka, Jakarta. pp 6, 37, 373 -37 4.

Koul, L., Walia, S., Dhaliwal, G.S. 2008. Essential oils as green pesticides: potential and consstraints, Biopestic. Int., 4(1) 63- 84.

Li, R.C., & Tang, M.C. 2004. Post antibiotic effect induced by an antibiotic combination influence of mode, sequence and interval of exposure. J. Antimicrob. Chemother. 54: 904- 908.

Mulyani, S. 2014a, Aktivitas larvasida granul minyak cengkeh, serai, dan jeruk terhadap larva nyamuk Aedes aegypti instar III, Laporan Penelitian, Fakultas Farmasi UGM, Yogyakarta.

Mulyani, S. 2014b. Granul minyak serai dapur sebagai larvasida nyamuk Aedes aegypti, Majalah Obat Tradisional, 19(3): 139-142.

Price, S., & Price, L. 1997. Aromaterapi Bagi Profesi Kesehatan, Cetakan I, diterjemahkan oleh Andry, H., EGC, Jakarta. pp. 29

Regalado, A., R.Y. Perry, B., & Yulfi, Z. 2011. Minyak atsiri dari daun dan batang tanaman dua spesies genus cymbopogon, gamili gramineae sebagai insektisida alami dan antibakteri, Prosiding Skripsi Semester Genap 2010/2011, Jurusan Kimia, Fakultas MIPA, Institut Teknologi Sepuluh Nopember.

Wasitaatmadja, S M,. 1997. Penuntun Ilmu Kosmetik Medik. UI Press, Jakarta. pp. 142- 143.



DOI: https://doi.org/10.22146/tradmedj.10693

Article Metrics

Abstract views : 1613 | views : 2157

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2016 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada